SRR1547604_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1547604_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1996991
Sequences flagged as poor quality	0
Sequence length	51
%GC	42

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17466	0.874615859560709	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4826	0.24166358286041348	No Hit
GAGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCT	4097	0.20515866120578408	Illumina PCR Primer Index 7 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3802	0.1903864363935541	No Hit
AAGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCT	3442	0.17235931458879886	TruSeq Adapter, Index 23 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	2884	0.14441727579142818	Illumina PCR Primer Index 7 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT	2527	0.12654038000171258	TruSeq Adapter, Index 23 (96% over 26bp)
AAGAGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTT	2508	0.12558894857312827	TruSeq Adapter, Index 23 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	2110	0.1056589639112044	Illumina PCR Primer Index 7 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTCACG	35	1.2125383E-7	45.000004	1
TAGCGAT	55	1.8189894E-12	45.000004	12
TCGATTG	75	0.0	45.000004	1
CGACACT	35	1.2125383E-7	45.000004	14
TATCTCC	45	3.8562575E-10	45.000004	35
ACTACCG	35	1.2125383E-7	45.000004	21
CTACCGT	35	1.2125383E-7	45.000004	22
GGCGAAT	35	1.2125383E-7	45.000004	9
TGTACGT	35	1.2125383E-7	45.000004	12
TAAGCGT	45	3.8562575E-10	45.000004	12
GAGATAT	55	1.8189894E-12	45.000004	9
AAATGCG	45	3.8562575E-10	45.000004	1
TCCGGCA	20	7.034538E-4	45.0	21
GTCATCG	20	7.034538E-4	45.0	9
TGTTTCG	20	7.034538E-4	45.0	1
AACGTAT	25	3.891843E-5	45.0	33
CGTGGCA	25	3.891843E-5	45.0	23
CCTTACT	40	6.8193913E-9	45.0	34
TACCCTT	20	7.034538E-4	45.0	34
ACACGGT	25	3.891843E-5	45.0	34