##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547604_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1996991
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.297017362622064	31.0	31.0	33.0	30.0	34.0
2	31.674527827115895	31.0	31.0	34.0	30.0	34.0
3	31.72668279426397	31.0	31.0	34.0	30.0	34.0
4	35.531049463918464	37.0	35.0	37.0	33.0	37.0
5	35.44538758562257	37.0	35.0	37.0	33.0	37.0
6	35.49842237646539	37.0	35.0	37.0	33.0	37.0
7	35.88277413368413	37.0	35.0	37.0	35.0	37.0
8	35.925425302367415	37.0	35.0	37.0	35.0	37.0
9	37.79305565222878	39.0	38.0	39.0	35.0	39.0
10	37.17156712273616	39.0	37.0	39.0	33.0	39.0
11	36.77358535917288	39.0	35.0	39.0	32.0	39.0
12	35.69456547375526	37.0	35.0	39.0	32.0	39.0
13	35.27737881642932	37.0	35.0	39.0	30.0	39.0
14	36.15747692403221	38.0	35.0	40.0	31.0	41.0
15	36.531663387566596	38.0	35.0	40.0	31.0	41.0
16	36.75277705307635	38.0	35.0	41.0	32.0	41.0
17	36.67911823338213	38.0	35.0	40.0	32.0	41.0
18	36.60069774976452	37.0	35.0	40.0	32.0	41.0
19	36.489750830123924	37.0	35.0	40.0	32.0	41.0
20	36.23065652273846	36.0	35.0	40.0	31.0	41.0
21	36.0229986013958	36.0	35.0	40.0	31.0	41.0
22	35.935785389117925	36.0	35.0	40.0	31.0	41.0
23	35.94683701629101	36.0	35.0	40.0	31.0	41.0
24	35.82454052121417	35.0	34.0	40.0	31.0	41.0
25	35.74398131989578	35.0	34.0	40.0	31.0	41.0
26	35.62476445812725	35.0	34.0	40.0	30.0	41.0
27	35.5844988785628	35.0	34.0	40.0	30.0	41.0
28	35.612366805859416	36.0	34.0	40.0	30.0	41.0
29	35.62119308499638	36.0	35.0	40.0	30.0	41.0
30	35.621970254247515	36.0	35.0	40.0	30.0	41.0
31	35.3954855079467	36.0	34.0	40.0	30.0	41.0
32	35.15347189847125	35.0	34.0	40.0	29.0	41.0
33	34.92872727017798	35.0	34.0	40.0	28.0	41.0
34	34.80571319550263	35.0	34.0	40.0	27.0	41.0
35	34.59133566450725	35.0	34.0	40.0	26.0	41.0
36	34.404588703704725	35.0	34.0	40.0	24.0	41.0
37	34.36102015482293	35.0	34.0	40.0	24.0	41.0
38	34.315602323696	35.0	33.0	40.0	24.0	41.0
39	34.304398467494345	35.0	34.0	40.0	24.0	41.0
40	34.186487069796506	35.0	34.0	40.0	23.0	41.0
41	34.14707878002455	35.0	34.0	40.0	23.0	41.0
42	34.095995925870476	35.0	33.0	40.0	23.0	41.0
43	34.0107391570618	35.0	33.0	40.0	23.0	41.0
44	33.87334444672009	35.0	33.0	40.0	23.0	41.0
45	33.75640801585986	35.0	33.0	40.0	23.0	41.0
46	33.69809077757486	35.0	33.0	40.0	23.0	41.0
47	33.67053131436246	35.0	33.0	40.0	23.0	41.0
48	33.5888929895027	35.0	33.0	40.0	23.0	41.0
49	33.59241929482907	35.0	33.0	39.0	23.0	41.0
50	33.45481226505277	35.0	33.0	39.0	22.0	41.0
51	32.994191761505185	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	3.0
13	8.0
14	16.0
15	41.0
16	145.0
17	343.0
18	821.0
19	1736.0
20	3202.0
21	5252.0
22	8359.0
23	12817.0
24	20054.0
25	29435.0
26	38389.0
27	42667.0
28	43726.0
29	48079.0
30	56378.0
31	68338.0
32	84801.0
33	111775.0
34	204441.0
35	365321.0
36	125494.0
37	141046.0
38	204741.0
39	379374.0
40	187.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.422220230336546	24.876226282441934	29.079450032574005	15.622103454647519
2	30.061227116196314	26.765719024272016	30.30163881559807	12.871415043933599
3	27.94774738594215	24.794553405598723	34.301957294749954	12.955741913709176
4	23.680076675358073	28.571185348356604	33.74391772421608	14.004820252069239
5	22.949026810836905	31.795836836520547	32.0216265371251	13.233509815517447
6	20.37946089892243	40.59913139318104	28.566578417228722	10.45482929066781
7	85.43027985604341	3.466415221701049	9.4216248345636	1.6816800876919324
8	85.31250266025235	3.133614523049929	8.266136402217136	3.2877464144805866
9	81.81594208486668	4.824107870290852	10.74591723247626	2.614032812366205
10	51.192469069715386	24.769816188455533	15.0777845268206	8.95993021500848
11	45.31883218301935	18.82156704762315	24.322142663637443	11.537458105720056
12	45.32839657264354	20.21376160433372	22.95112997504746	11.506711847975279
13	20.67430449110687	44.51186810556482	22.676266442863287	12.13756096046502
14	12.15548793159308	45.900206861222706	26.02565559884847	15.918649608335741
15	10.046765358481837	27.043086323373515	49.47648737525607	13.433660942888576
16	11.510617724366309	21.01331453171296	49.48700319630885	17.989064547611882
17	12.40806793821304	23.500857039415802	28.359166365797346	35.731908656573815
18	18.142895987012462	26.079436512232657	36.55013968515632	19.22752781559857
19	25.633665850271736	30.294628268229552	24.53431187221174	19.537394009286974
20	29.49923159393307	24.103714037769823	28.203582289554635	18.193472078742467
21	18.143697192426007	32.900749177137	29.23728749904231	19.71826613139468
22	20.455875865239253	26.30732937704777	26.308380959153045	26.928413798559937
23	16.98850921210962	33.002502264657174	25.045380775376554	24.96360774785665
24	17.24885089617329	24.85874998935899	42.73028771787154	15.162111396596178
25	13.806722213570316	29.260822908065183	36.707676699594536	20.22477817876996
26	13.443726085896232	39.172234626996314	27.718151959623256	19.6658873274842
27	14.193303825605625	39.885307445051076	28.352255969105517	17.56913276023778
28	11.444918880455646	32.59899518826074	40.10543863242248	15.850647298861137
29	11.117926921052723	25.2814859956805	40.4186598737801	23.181927209486673
30	15.201270311183174	37.38975288321279	31.313761554258377	16.09521525134565
31	26.77653529735487	32.81306725969221	23.893347541376002	16.51704990157692
32	27.621156029246002	29.690469311078516	25.580485840947702	17.107888818727776
33	25.203919296581706	32.17405586705198	23.729000280922648	18.893024555443667
34	16.30307798082215	29.601735811528446	28.14529459571926	25.94989161193015
35	16.19396381856503	27.529868687440256	33.37025554947418	22.905911944520533
36	27.043687227433672	27.129616508036342	27.49080992352995	18.335886341000034
37	16.034724242623028	34.09194132572455	33.074160073831074	16.799174357821343
38	15.943486976155627	33.216474185411954	30.074597231534845	20.76544160689758
39	16.129416707436338	33.7754651873744	30.339045093342936	19.756073011846322
40	23.275317715502975	28.385155466399198	27.48319847210128	20.856328345996552
41	14.267265100343469	24.721042808906	32.27590910524884	28.73578298550169
42	19.87645412523141	24.730356821838456	26.328160717799932	29.065028335130204
43	20.44520981817144	27.146692198412513	27.049345740666837	25.358752242749215
44	17.226567370609082	31.473802335613932	27.952604693761764	23.347025600015222
45	14.449088653879762	41.637794061165025	22.031296084959823	21.881821199995393
46	20.475705699224484	37.568471765771605	25.08974752515159	16.86607500985232
47	18.021012613476977	29.474644602804922	29.229676047613633	23.274666736104468
48	19.350262469885944	24.625098460634025	35.55979971867675	20.46483935080328
49	19.13599009710109	24.287740906193367	33.867653885270386	22.708615111435154
50	16.53457626999821	32.53204446089141	31.75237144283575	19.18100782627463
51	14.587546964407952	34.152582560462214	26.470424754042455	24.789445721087375
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1210.0
1	1625.5
2	2041.0
3	12526.5
4	23012.0
5	16336.5
6	9661.0
7	10302.5
8	10944.0
9	12527.5
10	14111.0
11	14909.0
12	15707.0
13	15403.0
14	15099.0
15	14319.0
16	13539.0
17	12886.0
18	12233.0
19	11494.5
20	10756.0
21	10408.5
22	10061.0
23	9912.5
24	9764.0
25	10391.0
26	14000.0
27	16982.0
28	22250.0
29	27518.0
30	31116.5
31	34715.0
32	42861.5
33	51008.0
34	60780.5
35	70553.0
36	70211.0
37	69869.0
38	80907.5
39	91946.0
40	127402.5
41	162859.0
42	206214.0
43	249569.0
44	262327.5
45	275086.0
46	256753.5
47	238421.0
48	210086.0
49	181751.0
50	153191.5
51	124632.0
52	103265.5
53	81899.0
54	68700.0
55	55501.0
56	46497.0
57	37493.0
58	30455.0
59	23417.0
60	20378.5
61	17340.0
62	14408.0
63	11476.0
64	9053.5
65	6631.0
66	4942.5
67	3254.0
68	2744.5
69	2235.0
70	1766.0
71	1297.0
72	1033.5
73	770.0
74	722.0
75	495.0
76	316.0
77	273.5
78	231.0
79	210.0
80	189.0
81	141.0
82	93.0
83	82.5
84	72.0
85	48.0
86	24.0
87	17.5
88	11.0
89	7.0
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1996991.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.233521547242553
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.95765910037407	14.801667945323915
2	10.040676750515175	3.8623514525986185
3	3.8671518167833328	2.231368433836812
4	1.9364855766502265	1.4898174825770618
5	1.128813243427528	1.0855526920138054
6	0.739844446369598	0.853788846051444
7	0.5299684131545928	0.7135211225636761
8	0.3751217233911391	0.5771929399745788
9	0.2651121414052669	0.45891360757384336
>10	2.154995992106153	8.918140264966707
>50	0.6489139146269269	9.228558001505828
>100	1.3310182278013634	50.71828011128952
>500	0.019759771789128806	2.313389665208566
>1k	0.004215417981680812	1.8623970027251315
>5k	0.0	0.0
>10k+	2.6346362385505076E-4	0.8850604317905245
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17466	0.874615859560709	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4826	0.24166358286041348	No Hit
GAGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCT	4097	0.20515866120578408	Illumina PCR Primer Index 7 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3802	0.1903864363935541	No Hit
AAGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCT	3442	0.17235931458879886	TruSeq Adapter, Index 23 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	2884	0.14441727579142818	Illumina PCR Primer Index 7 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT	2527	0.12654038000171258	TruSeq Adapter, Index 23 (96% over 26bp)
AAGAGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTT	2508	0.12558894857312827	TruSeq Adapter, Index 23 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	2110	0.1056589639112044	Illumina PCR Primer Index 7 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.007533834654237E-5	0.0	0.0	0.13905921458834816	0.0
2	5.007533834654237E-5	0.0	0.0	0.5471732221126685	0.0
3	5.007533834654237E-5	0.0	0.0	0.8216862269284138	0.0
4	5.007533834654237E-5	0.0	0.0	1.5932971155102853	0.0
5	5.007533834654237E-5	0.0	0.0	2.556596399282721	0.0
6	5.007533834654237E-5	0.0	0.0	3.661208287869099	0.0
7	5.007533834654237E-5	0.0	0.0	4.142282063364331	0.0
8	5.007533834654237E-5	0.0	0.0	5.3995736585693175	0.0
9	5.007533834654237E-5	0.0	0.0	5.729770439626418	0.0
10	5.007533834654237E-5	0.0	0.0	6.36307324369514	0.0
11	5.007533834654237E-5	0.0	0.0	7.249306581752246	0.0
12	1.0015067669308474E-4	0.0	0.0	7.904091705971634	0.0
13	1.0015067669308474E-4	0.0	0.0	8.161929623117981	0.0
14	1.0015067669308474E-4	0.0	0.0	8.274398833044316	0.0
15	1.0015067669308474E-4	0.0	0.0	8.445305962821065	0.0
16	1.0015067669308474E-4	0.0	0.0	8.852118011548374	0.0
17	1.0015067669308474E-4	0.0	0.0	9.292079934261096	0.0
18	1.0015067669308474E-4	0.0	0.0	9.841005793215894	0.0
19	1.0015067669308474E-4	0.0	0.0	10.113415633821084	0.0
20	1.0015067669308474E-4	0.0	0.0	10.393336775178255	0.0
21	1.0015067669308474E-4	0.0	0.0	10.783223359544435	0.0
22	1.0015067669308474E-4	0.0	0.0	11.164547061053355	0.0
23	1.0015067669308474E-4	0.0	0.0	11.58302666361541	0.0
24	1.0015067669308474E-4	0.0	0.0	11.898200843168548	0.0
25	1.0015067669308474E-4	0.0	0.0	12.160345239412697	0.0
26	1.0015067669308474E-4	0.0	0.0	12.39374639144593	0.0
27	1.0015067669308474E-4	0.0	0.0	12.618133982576786	0.0
28	1.0015067669308474E-4	0.0	0.0	12.859697414760507	0.0
29	1.0015067669308474E-4	0.0	0.0	13.127099721531044	0.0
30	1.0015067669308474E-4	0.0	0.0	13.472319104092106	0.0
31	1.0015067669308474E-4	0.0	0.0	13.777678517329322	0.0
32	1.0015067669308474E-4	0.0	0.0	14.034865455077163	0.0
33	1.0015067669308474E-4	0.0	0.0	14.285993276885074	0.0
34	1.0015067669308474E-4	0.0	0.0	14.564812760798622	0.0
35	1.0015067669308474E-4	0.0	0.0	14.979086034939566	0.0
36	1.0015067669308474E-4	0.0	0.0	15.260359210432094	0.0
37	1.0015067669308474E-4	0.0	0.0	15.542083063969743	0.0
38	1.0015067669308474E-4	0.0	0.0	15.78760244788284	0.0
39	1.0015067669308474E-4	0.0	0.0	16.045941118412653	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTCACG	35	1.2125383E-7	45.000004	1
TAGCGAT	55	1.8189894E-12	45.000004	12
TCGATTG	75	0.0	45.000004	1
CGACACT	35	1.2125383E-7	45.000004	14
TATCTCC	45	3.8562575E-10	45.000004	35
ACTACCG	35	1.2125383E-7	45.000004	21
CTACCGT	35	1.2125383E-7	45.000004	22
GGCGAAT	35	1.2125383E-7	45.000004	9
TGTACGT	35	1.2125383E-7	45.000004	12
TAAGCGT	45	3.8562575E-10	45.000004	12
GAGATAT	55	1.8189894E-12	45.000004	9
AAATGCG	45	3.8562575E-10	45.000004	1
TCCGGCA	20	7.034538E-4	45.0	21
GTCATCG	20	7.034538E-4	45.0	9
TGTTTCG	20	7.034538E-4	45.0	1
AACGTAT	25	3.891843E-5	45.0	33
CGTGGCA	25	3.891843E-5	45.0	23
CCTTACT	40	6.8193913E-9	45.0	34
TACCCTT	20	7.034538E-4	45.0	34
ACACGGT	25	3.891843E-5	45.0	34
>>END_MODULE