FastQCFastQC Report
Thu 26 May 2016
SRR1547603_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547603_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2295113
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204810.8923743623952285No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC84960.37017785180947516No Hit
GAGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCT27920.12164978369256765No Hit
AAGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCT23880.10404716456226773No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGGT253.892025E-545.00000416
CGAACCG253.892025E-545.00000429
CATTGCG253.892025E-545.0000041
TTCACGC253.892025E-545.00000441
TCGATTG253.892025E-545.0000041
CACGTTA253.892025E-545.00000430
ATCGGTC253.892025E-545.00000422
CTTAACG351.2126293E-745.01
GTCGCGC207.034758E-445.037
TCGTTAG800.045.01
ACGCCCG351.2126293E-745.029
CGAGTCA406.8193913E-945.021
CGACGGA406.8193913E-945.029
CGGTTTA406.8193913E-945.011
CTATACG453.8562575E-1045.01
CGCGCCA351.2126293E-745.026
TATATCG406.8193913E-945.015
CGACATA207.034758E-445.029
ATCGTAG453.8562575E-1045.01
ATATCGT351.2126293E-745.016