##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547603_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2295113
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.305883414019267	31.0	31.0	33.0	30.0	34.0
2	31.70000300638792	31.0	31.0	34.0	30.0	34.0
3	31.77265433118108	31.0	31.0	34.0	30.0	34.0
4	35.55979945214027	37.0	35.0	37.0	33.0	37.0
5	35.477062785143914	37.0	35.0	37.0	33.0	37.0
6	35.5226679470684	37.0	35.0	37.0	33.0	37.0
7	35.91060222307137	37.0	35.0	37.0	35.0	37.0
8	35.952678582710305	37.0	35.0	37.0	35.0	37.0
9	37.80296307850637	39.0	38.0	39.0	35.0	39.0
10	37.15270097812177	39.0	37.0	39.0	33.0	39.0
11	36.80263716862743	39.0	35.0	39.0	32.0	39.0
12	35.78414047587199	37.0	35.0	39.0	32.0	39.0
13	35.3388634895101	37.0	35.0	39.0	30.0	39.0
14	36.24737518370555	38.0	35.0	41.0	31.0	41.0
15	36.60876131153455	38.0	35.0	41.0	31.0	41.0
16	36.79749319532415	38.0	35.0	41.0	32.0	41.0
17	36.71481839891979	38.0	35.0	41.0	32.0	41.0
18	36.61090107545903	38.0	35.0	40.0	32.0	41.0
19	36.50635241053491	37.0	35.0	40.0	31.0	41.0
20	36.30268357157142	36.0	35.0	40.0	31.0	41.0
21	36.0849322015953	36.0	35.0	40.0	31.0	41.0
22	35.97804857538605	36.0	35.0	40.0	31.0	41.0
23	35.98556323806279	36.0	35.0	40.0	31.0	41.0
24	35.90769081958056	36.0	35.0	40.0	31.0	41.0
25	35.8057886474435	36.0	34.0	40.0	31.0	41.0
26	35.674010386416704	36.0	34.0	40.0	30.0	41.0
27	35.61397238393055	35.0	34.0	40.0	30.0	41.0
28	35.64556952097784	36.0	34.0	40.0	30.0	41.0
29	35.724533388987815	36.0	35.0	40.0	30.0	41.0
30	35.72890049422403	36.0	35.0	40.0	30.0	41.0
31	35.56625926479437	36.0	34.0	40.0	30.0	41.0
32	35.3397675844283	36.0	34.0	40.0	29.0	41.0
33	35.153842098406486	36.0	34.0	40.0	29.0	41.0
34	35.05131119905643	36.0	34.0	40.0	28.0	41.0
35	34.87110743566875	36.0	34.0	40.0	27.0	41.0
36	34.699084097384315	35.0	34.0	40.0	25.0	41.0
37	34.633059025851885	35.0	34.0	40.0	25.0	41.0
38	34.59173818456869	35.0	34.0	40.0	25.0	41.0
39	34.574899362253625	36.0	34.0	40.0	25.0	41.0
40	34.458842331510475	36.0	34.0	40.0	24.0	41.0
41	34.41208297804945	36.0	34.0	40.0	24.0	41.0
42	34.37078087222721	36.0	34.0	40.0	24.0	41.0
43	34.31128489098358	35.0	33.0	40.0	24.0	41.0
44	34.175890685992364	35.0	34.0	40.0	23.0	41.0
45	34.10227557423099	35.0	33.0	40.0	23.0	41.0
46	34.085342638902745	35.0	34.0	40.0	23.0	41.0
47	34.072450463223376	35.0	33.0	40.0	23.0	41.0
48	33.97698152552837	35.0	33.0	40.0	23.0	41.0
49	33.98007244087764	35.0	33.0	40.0	24.0	41.0
50	33.857259315772254	35.0	33.0	40.0	24.0	41.0
51	33.42935750875883	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	0.0
12	3.0
13	1.0
14	17.0
15	43.0
16	152.0
17	423.0
18	1080.0
19	2029.0
20	3705.0
21	6043.0
22	9281.0
23	13822.0
24	20230.0
25	30046.0
26	40421.0
27	47243.0
28	50787.0
29	55250.0
30	64005.0
31	77169.0
32	95119.0
33	125168.0
34	228972.0
35	389348.0
36	142923.0
37	170103.0
38	255452.0
39	466100.0
40	175.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.71504540299323	24.260461249620388	29.625905129725638	15.398588217660741
2	30.018260538805713	26.05897835967118	30.68258512761681	13.240175973906295
3	27.67114298947372	24.841565535117443	34.0176714610566	13.469620014352234
4	23.996247679308162	27.480694850318915	33.71481055616869	14.808246914204224
5	22.172154486511122	31.76196553285176	32.521100268265656	13.54477971237146
6	20.717629153771515	39.92644370887185	29.080223936686338	10.275703200670295
7	86.3984910546888	3.7195989914222087	8.451435724515525	1.4304742293734556
8	87.59067636321176	2.6171260412885986	7.580193219244542	2.2120043762551127
9	83.97285885270136	4.248984690514149	9.601183035432243	2.1769734213522383
10	50.691447436357166	23.35013570137941	14.937913732352175	11.020503129911251
11	45.63505152033909	20.425617387902033	22.214766767475066	11.724564324283815
12	41.92281600078079	20.45280559170725	24.29139654561671	13.332981861895254
13	20.341569238638797	41.00726195180804	25.246164350077756	13.405004459475418
14	14.325482013303919	43.80368199735699	27.8331393704798	14.037696618859288
15	12.697849735503219	24.57138275980311	50.124155106959876	12.606612397733793
16	14.948240021297426	20.01783790166323	49.06621155472519	15.967710522314151
17	15.458715976076123	20.9643272466323	29.07355759825333	34.50339917903824
18	20.407666202056284	24.181467317731197	36.65667006373978	18.75419641647274
19	27.605699588647703	25.190306533926655	28.60181611972918	18.602177757696463
20	30.668860313195907	23.920042281142585	27.546966097094128	17.864131308567377
21	20.855748714769167	28.3434846127402	31.081345450093306	19.71942122239733
22	21.41288903857893	23.40939204300616	29.265792141824825	25.911926776590082
23	17.54641274743335	29.99725939419976	27.529537761321553	24.92679009704533
24	19.542087905911387	23.48978024175716	40.55199025058897	16.416141601742485
25	15.815212584304128	25.24224297452892	37.73888257353777	21.203661867629176
26	15.570823745933208	36.08214497499687	29.313545781841675	19.03348549722824
27	16.596132739433745	36.4995100459106	30.867543341003252	16.03681387365241
28	13.765466014091682	30.271189261705196	40.70100252144448	15.262342202758644
29	14.035430935208854	25.221416113280696	40.22695178843046	20.516201163079987
30	16.12521910685879	32.95576296243366	33.697556503753844	17.221461426953706
31	27.482916963129924	27.869216025529024	26.818853799355413	17.829013211985643
32	27.69780834320576	28.86293616044177	28.07604680031005	15.363208696042415
33	25.986040774462953	29.654095462837777	25.454258679202287	18.90560508349698
34	17.673683169412573	30.36421300389131	28.200964396959975	23.76113942973614
35	17.4121709911451	27.696501218022817	32.11323364034799	22.778094150484094
36	27.713232420364488	24.785533435608617	29.547216193712465	17.95401795031443
37	17.82413327796932	32.5904650446405	32.2385433745528	17.346858302837376
38	17.88277962784403	33.19095835368454	25.67359428490013	23.25266773357129
39	18.639910104644088	32.326556470204295	30.115728506613838	18.91780491853778
40	22.840966871783657	27.89666565437083	26.905777624021127	22.35658984982439
41	15.817478267954563	25.146866407013512	30.699752038352795	28.33590328667913
42	21.30579191525646	25.194881472066953	27.176831816124086	26.322494796552505
43	21.820668524817734	25.033974362046663	27.84660275986411	25.298754353271498
44	17.423542980236704	31.547161294454785	29.963622706158695	21.06567301914982
45	16.552431187484014	38.23633084732647	24.540839601361675	20.67039836382784
46	21.624381893179116	33.594860035213955	26.31508775384916	18.465670317757773
47	20.053217423281556	29.200828020232557	27.451240962863267	23.294713593622625
48	22.04802116497096	24.788147685974504	32.7810438963136	20.382787252740933
49	20.335556462797257	23.26669754386821	34.25251828559204	22.145227707742496
50	18.45307834516209	32.63643228024067	29.360123009193885	19.55036636540336
51	17.217235055528857	33.797813005285576	26.102331344905462	22.882620594280105
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1275.0
1	2190.0
2	3105.0
3	16621.5
4	30138.0
5	21111.5
6	12085.0
7	12361.5
8	12638.0
9	13788.5
10	14939.0
11	15483.5
12	16028.0
13	15504.0
14	14980.0
15	13826.5
16	12673.0
17	11941.5
18	11210.0
19	10484.5
20	9759.0
21	10627.0
22	11495.0
23	11390.0
24	11285.0
25	13198.0
26	16587.5
27	18064.0
28	21368.5
29	24673.0
30	31627.5
31	38582.0
32	46600.5
33	54619.0
34	56096.5
35	57574.0
36	67722.5
37	77871.0
38	93482.0
39	109093.0
40	143575.5
41	178058.0
42	219554.0
43	261050.0
44	268101.0
45	275152.0
46	268167.0
47	261182.0
48	229138.0
49	197094.0
50	175368.0
51	153642.0
52	137375.5
53	121109.0
54	104923.5
55	88738.0
56	74478.0
57	60218.0
58	53043.0
59	45868.0
60	39504.0
61	33140.0
62	28519.5
63	23899.0
64	19911.5
65	15924.0
66	12421.5
67	8919.0
68	7301.0
69	5683.0
70	4479.0
71	3275.0
72	2876.5
73	2478.0
74	1869.0
75	892.0
76	524.0
77	401.5
78	279.0
79	223.5
80	168.0
81	179.0
82	190.0
83	111.5
84	33.0
85	18.5
86	4.0
87	6.0
88	8.0
89	8.0
90	8.0
91	6.5
92	5.0
93	3.5
94	2.0
95	4.0
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2295113.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.556694161403986
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.9914828950036	12.91281129486945
2	9.458792294490687	3.1321266231225393
3	3.522716996926646	1.7497364380588194
4	1.788904020015193	1.1847334697399066
5	1.0176869171302119	0.8424765519493501
6	0.6319504322767757	0.627780601942417
7	0.4319710971581935	0.5006409339550035
8	0.31866317431902086	0.42208069741617543
9	0.24773050433524196	0.3691438375256069
>10	1.9248374305268707	7.33840566212632
>50	0.9018534845839443	11.039605786055699
>100	1.7425988242358585	55.92325926519671
>500	0.017076454562223137	1.8646019464380066
>1k	0.003201835230416838	0.812494290155606
>5k	2.668196025347365E-4	0.37532359118975794
>10k+	2.668196025347365E-4	0.9047790102586433
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20481	0.8923743623952285	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	8496	0.37017785180947516	No Hit
GAGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCT	2792	0.12164978369256765	No Hit
AAGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCT	2388	0.10404716456226773	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06426698816136722	0.0
2	0.0	0.0	0.0	0.26517212877971585	0.0
3	0.0	0.0	0.0	0.39388038846017603	0.0
4	0.0	0.0	0.0	0.8245781362399149	0.0
5	0.0	0.0	0.0	1.3264706356506193	0.0
6	0.0	0.0	0.0	1.9360266792964007	0.0
7	0.0	0.0	0.0	2.1971031491695614	0.0
8	0.0	0.0	0.0	2.8977222472270427	0.0
9	0.0	0.0	0.0	3.0764934014142225	0.0
10	0.0	0.0	0.0	3.4560389837014562	0.0
11	0.0	0.0	0.0	3.9888667791084798	0.0
12	0.0	0.0	0.0	4.4010469201298585	0.0
13	0.0	0.0	0.0	4.568010376831119	0.0
14	0.0	0.0	0.0	4.626438872508674	0.0
15	0.0	0.0	0.0	4.730268182873784	0.0
16	0.0	0.0	0.0	4.983981180883033	0.0
17	0.0	0.0	0.0	5.259043890213684	0.0
18	0.0	0.0	0.0	5.648262198854697	0.0
19	0.0	0.0	0.0	5.828558332421976	0.0
20	0.0	0.0	0.0	6.014997954349089	0.0
21	0.0	0.0	0.0	6.288884251015092	0.0
22	0.0	0.0	0.0	6.551529271107785	0.0
23	0.0	0.0	0.0	6.845109587196796	0.0
24	0.0	0.0	0.0	7.059695971396615	0.0
25	0.0	0.0	0.0	7.238815692299246	0.0
26	0.0	0.0	0.0	7.421421080356392	0.0
27	0.0	0.0	0.0	7.5755311394253795	0.0
28	0.0	0.0	0.0	7.746110975799449	0.0
29	0.0	0.0	0.0	7.938868369444119	0.0
30	0.0	0.0	0.0	8.176024448469422	0.0
31	0.0	0.0	0.0	8.414531223517098	0.0
32	0.0	0.0	0.0	8.614085668113074	0.0
33	0.0	0.0	0.0	8.818389334207074	0.0
34	4.3570839431435405E-5	0.0	0.0	9.03349856848007	0.0
35	4.3570839431435405E-5	0.0	0.0	9.309171269562762	0.0
36	4.3570839431435405E-5	0.0	0.0	9.55464937891947	0.0
37	4.3570839431435405E-5	0.0	0.0	9.786184819658118	0.0
38	4.3570839431435405E-5	0.0	0.0	10.002252612398605	0.0
39	4.3570839431435405E-5	0.0	0.0	10.224768889374946	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGGT	25	3.892025E-5	45.000004	16
CGAACCG	25	3.892025E-5	45.000004	29
CATTGCG	25	3.892025E-5	45.000004	1
TTCACGC	25	3.892025E-5	45.000004	41
TCGATTG	25	3.892025E-5	45.000004	1
CACGTTA	25	3.892025E-5	45.000004	30
ATCGGTC	25	3.892025E-5	45.000004	22
CTTAACG	35	1.2126293E-7	45.0	1
GTCGCGC	20	7.034758E-4	45.0	37
TCGTTAG	80	0.0	45.0	1
ACGCCCG	35	1.2126293E-7	45.0	29
CGAGTCA	40	6.8193913E-9	45.0	21
CGACGGA	40	6.8193913E-9	45.0	29
CGGTTTA	40	6.8193913E-9	45.0	11
CTATACG	45	3.8562575E-10	45.0	1
CGCGCCA	35	1.2126293E-7	45.0	26
TATATCG	40	6.8193913E-9	45.0	15
CGACATA	20	7.034758E-4	45.0	29
ATCGTAG	45	3.8562575E-10	45.0	1
ATATCGT	35	1.2126293E-7	45.0	16
>>END_MODULE