FastQCFastQC Report
Thu 26 May 2016
SRR1547602_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547602_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2737480
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176470.6446439791340941No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACCG750.042.01
TAGCACG700.041.7857171
CGTTTTT97850.041.780791
AGGGCGA18550.039.4204836
CGACGGT1150.039.13043628
TAAGGGA44850.038.829434
TATAGCG1450.038.7931061
CGGTAGT3050.038.36065712
TCGTCCC9650.038.23834238
ACGGGAT13900.037.5539555
GTAGCGT301.1403339E-437.50000434
AGGGATT51200.037.4414066
ATAGGGA43100.037.3259854
CGCGAGG3150.037.1428572
CCTCGTC9950.037.08542336
GAGGGAT48200.036.7842335
CTCGTCC10350.036.73913237
CGTAAGG4900.036.7346952
CGTTAGG5100.036.617652
GGGCGAT39800.036.576637
Produced by FastQC (version 0.11.5)