##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547602_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2737480
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.40223161447755	31.0	31.0	33.0	30.0	34.0
2	31.830932463433523	31.0	31.0	34.0	30.0	34.0
3	31.95892280491547	31.0	31.0	34.0	30.0	34.0
4	35.74317693645251	37.0	35.0	37.0	35.0	37.0
5	35.67472200710142	37.0	35.0	37.0	33.0	37.0
6	35.71959685550214	37.0	35.0	37.0	33.0	37.0
7	35.997621535134506	37.0	35.0	37.0	35.0	37.0
8	36.03123748849307	37.0	35.0	37.0	35.0	37.0
9	37.869803980303054	39.0	38.0	39.0	35.0	39.0
10	37.31666678843316	39.0	37.0	39.0	34.0	39.0
11	37.000163654163686	39.0	37.0	39.0	33.0	39.0
12	36.638400280549995	39.0	35.0	39.0	33.0	39.0
13	36.54360470213481	39.0	35.0	39.0	32.0	39.0
14	37.63318270818417	40.0	36.0	41.0	33.0	41.0
15	37.79196742989903	40.0	36.0	41.0	33.0	41.0
16	37.84719303885325	40.0	36.0	41.0	33.0	41.0
17	37.75188786767392	40.0	36.0	41.0	33.0	41.0
18	37.6467605242778	39.0	36.0	41.0	33.0	41.0
19	37.60744407265076	39.0	36.0	41.0	33.0	41.0
20	37.48266909712582	39.0	35.0	41.0	33.0	41.0
21	37.33175438724666	39.0	35.0	41.0	32.0	41.0
22	37.28523021172757	39.0	35.0	41.0	32.0	41.0
23	37.20332787819454	39.0	35.0	41.0	32.0	41.0
24	37.119118678492626	39.0	35.0	41.0	32.0	41.0
25	37.027705773192864	39.0	35.0	41.0	32.0	41.0
26	36.926949968584246	39.0	35.0	41.0	32.0	41.0
27	36.84941186784926	38.0	35.0	40.0	31.0	41.0
28	36.86161542732732	39.0	35.0	40.0	31.0	41.0
29	36.87356693016935	39.0	35.0	41.0	31.0	41.0
30	36.885853777927146	39.0	35.0	41.0	31.0	41.0
31	36.821035404824876	39.0	35.0	41.0	31.0	41.0
32	36.71228502126043	39.0	35.0	41.0	31.0	41.0
33	36.58560573958531	39.0	35.0	41.0	31.0	41.0
34	36.475773338983295	39.0	35.0	41.0	30.0	41.0
35	36.28597834504727	39.0	35.0	41.0	30.0	41.0
36	36.20821595043617	39.0	35.0	41.0	30.0	41.0
37	36.1593249996347	39.0	35.0	41.0	30.0	41.0
38	36.08162032234026	38.0	35.0	41.0	30.0	41.0
39	36.007002425588496	38.0	35.0	41.0	29.0	41.0
40	35.94532197495507	38.0	35.0	41.0	29.0	41.0
41	35.86720195216038	38.0	35.0	40.0	29.0	41.0
42	35.852187047941904	38.0	35.0	40.0	29.0	41.0
43	35.818510820170374	38.0	35.0	40.0	29.0	41.0
44	35.698638894165434	38.0	35.0	40.0	28.0	41.0
45	35.600771512485935	38.0	35.0	40.0	28.0	41.0
46	35.567432456127534	38.0	34.0	40.0	28.0	41.0
47	35.51791026783757	38.0	34.0	40.0	28.0	41.0
48	35.386452503762584	38.0	34.0	40.0	27.0	41.0
49	35.31219223519441	38.0	34.0	40.0	27.0	41.0
50	35.233859242807256	38.0	34.0	40.0	27.0	41.0
51	34.801355261043	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	5.0
12	6.0
13	2.0
14	15.0
15	19.0
16	90.0
17	268.0
18	613.0
19	1279.0
20	2284.0
21	3989.0
22	6465.0
23	10089.0
24	15679.0
25	23290.0
26	32270.0
27	38845.0
28	42221.0
29	47621.0
30	56568.0
31	70514.0
32	89615.0
33	118444.0
34	193089.0
35	281622.0
36	208236.0
37	276467.0
38	437418.0
39	780230.0
40	225.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.85619620965267	23.768758127913266	28.81599134970849	15.559054312725573
2	30.651328959480985	25.625867586247203	30.898892411999356	12.823911042272455
3	27.896751757090467	26.244794482516763	32.36922278884229	13.489230971550478
4	25.234558791297108	28.70143343513012	32.06956763154434	13.994440142028436
5	22.98584829843506	32.70000146119789	30.439491795373847	13.874658444993205
6	22.31545070648918	40.6085158613031	26.688706401507957	10.387327030699769
7	89.85373409120797	2.7725134064906407	6.091770533483349	1.281981968818037
8	90.62086298347384	2.0778599295702618	5.823311951137542	1.4779651358183439
9	85.3111986206292	5.186448850767859	7.300838727588878	2.2015138010140713
10	41.302438739278465	36.41962681005889	12.65700571328375	9.620928737378904
11	28.99228487514064	24.320981340502946	29.656289726317635	17.03044405803878
12	30.219070093662786	22.664348232681153	29.05398395604717	18.062597717608895
13	23.060113681195844	28.716849072869937	29.78644592837208	18.436591317562137
14	18.482326811520082	31.16464777824861	30.574141180940135	19.778884229291176
15	17.143796484357875	27.70632114207227	37.2414775633064	17.908404810263452
16	21.47643087803381	25.323509212852695	35.613849233601705	17.586210675511786
17	21.20143343513012	25.31543609451028	29.828236188026946	23.654894282332656
18	21.642386428393998	25.878764411064193	32.91512632055759	19.56372283998422
19	24.96376229232725	27.228399842190626	28.385157151833074	19.422680713649047
20	26.230876572614232	26.8598126744305	28.775698817890905	18.133611935064366
21	23.809489019097857	27.039357365167966	30.39803030524424	18.75312331048994
22	23.36802460657247	23.821909201163113	30.422468839955	22.387597352309424
23	20.324933880795477	27.693462600640007	30.388276809328286	21.593326709236234
24	20.214065490889432	26.46514312433333	34.03772812952058	19.28306325525666
25	20.238394435758437	28.325357628183585	30.94532197495507	20.490925961102914
26	19.76244575302833	31.6521764542572	28.49109399886027	20.0942837938542
27	19.017052179376652	30.933157502520565	30.691840670982074	19.35794964712071
28	18.590601575171327	28.29142130718763	33.94917953738475	19.168797580256296
29	18.84693221502988	25.90773265923404	34.09946374037436	21.14587138536172
30	20.39262387305113	28.52791618568903	31.55135379984511	19.52810614141473
31	24.102970615310433	27.104928620483072	28.853690255271268	19.938410508935224
32	24.577238920467	27.333934859798063	28.965179654280583	19.12364656545436
33	23.747497698613323	27.552712713882844	28.19311921913585	20.50667036836799
34	20.989596271022982	26.977366044683432	30.23675058813215	21.796287096161432
35	21.132720604351444	27.2801993073922	30.91923959261803	20.667840495638323
36	24.618554290807605	26.893822055320953	28.92470447272674	19.562919181144704
37	20.980756023788302	29.582426172976607	30.058959334862724	19.377858468372374
38	21.295790288878823	29.694901880561687	28.355787074243466	20.653520756316027
39	20.768809269839412	29.805039671522714	29.27330975934071	20.152841299297165
40	23.261978169703525	27.465296550111784	28.84872218244517	20.424003097739526
41	19.372744275757267	27.36242821865365	30.072219705714744	23.192607799874338
42	21.461745839239008	27.537077896459515	28.199694609640986	22.801481654660492
43	21.45458596957786	27.611708578692813	28.7029311629674	22.230774288761925
44	20.5143781872379	29.34677148326198	29.19257857591654	20.946271753583588
45	19.579138477724037	30.623383549834156	28.25244385347108	21.545034118970733
46	21.114638280462323	29.97073951225214	28.630236567938393	20.284385639347136
47	20.785320805996758	27.79413913526309	29.749915981121323	21.670624077618832
48	21.38945307362976	27.011777254993646	30.973888393705156	20.624881277671435
49	21.087094700235255	26.552267048526385	31.0822362172509	21.27840203398746
50	19.875140640296916	29.526389233893944	29.810044274296065	20.78842585151307
51	19.65840846325818	29.503558016862225	28.95991934187647	21.878114178003127
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1251.0
1	2441.0
2	3631.0
3	16513.5
4	29396.0
5	20655.0
6	11914.0
7	11728.0
8	11542.0
9	12052.5
10	12563.0
11	12635.0
12	12707.0
13	12518.5
14	12330.0
15	11970.5
16	11611.0
17	11391.0
18	11171.0
19	10828.5
20	10486.0
21	11457.0
22	12428.0
23	14253.0
24	16078.0
25	18458.5
26	27494.5
27	34150.0
28	40830.5
29	47511.0
30	55646.0
31	63781.0
32	70951.0
33	78121.0
34	86656.5
35	95192.0
36	106138.5
37	117085.0
38	130772.0
39	144459.0
40	165638.0
41	186817.0
42	198570.5
43	210324.0
44	214413.5
45	218503.0
46	215894.5
47	213286.0
48	215566.5
49	217847.0
50	214253.5
51	210660.0
52	198799.0
53	186938.0
54	164001.0
55	141064.0
56	125768.5
57	110473.0
58	98012.0
59	85551.0
60	75559.5
61	65568.0
62	56069.5
63	46571.0
64	38901.0
65	31231.0
66	25360.5
67	19490.0
68	16691.5
69	13893.0
70	11372.0
71	8851.0
72	7000.5
73	5150.0
74	4242.5
75	2680.0
76	2025.0
77	1509.0
78	993.0
79	702.5
80	412.0
81	282.0
82	152.0
83	109.0
84	66.0
85	43.5
86	21.0
87	14.5
88	8.0
89	5.5
90	3.0
91	2.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2737480.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.057162539737334
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.47213092415541	13.808696978575483
2	7.298883553636001	2.6359425337324183
3	2.5356840846767428	1.373617789992991
4	1.4610259960512513	1.0552793554191635
5	0.8767867950205654	0.7916140835190856
6	0.6394602467058311	0.6928102567480638
7	0.5211781202612837	0.6587698620798941
8	0.41404183444738113	0.5981136562293902
9	0.36071173811460916	0.5862087436613997
>10	6.17765836880975	28.913006558402927
>50	2.593700308765022	33.32441546537294
>100	0.6452491083150493	14.4248191636104
>500	0.0030784597422249837	0.39204538328646377
>1k	2.0523064948166556E-4	0.09068296180186944
>5k	0.0	0.0
>10k+	2.0523064948166556E-4	0.6539772075674662
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17647	0.6446439791340941	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.037918085246284906	0.0
2	0.0	0.0	0.0	0.11474056431462513	0.0
3	0.0	0.0	0.0	0.19167263322471761	0.0
4	0.0	0.0	0.0	0.3182854303958385	0.0
5	0.0	0.0	0.0	0.5516752633809197	0.0
6	0.0	0.0	0.0	0.9210295600333153	0.0
7	0.0	0.0	0.0	1.1011221999795433	0.0
8	0.0	0.0	0.0	1.5368879407338136	0.0
9	0.0	0.0	0.0	1.7373277612987126	0.0
10	0.0	0.0	0.0	2.018023875973523	0.0
11	0.0	0.0	0.0	2.325058082616129	0.0
12	0.0	0.0	0.0	2.579964054531905	0.0
13	0.0	0.0	0.0	2.7032160965559564	0.0
14	0.0	0.0	0.0	2.772038517176381	0.0
15	0.0	0.0	0.0	2.840678288060552	0.0
16	0.0	0.0	0.0	2.969044522699709	0.0
17	0.0	0.0	0.0	3.123347019886903	0.0
18	0.0	0.0	0.0	3.2981428174817715	0.0
19	0.0	0.0	0.0	3.423002177184856	0.0
20	0.0	0.0	0.0	3.5453409705276386	0.0
21	0.0	0.0	0.0	3.706620687639727	0.0
22	0.0	0.0	0.0	3.889270423893508	0.0
23	0.0	0.0	0.0	4.078678200388679	0.0
24	0.0	0.0	0.0	4.2228253722401625	0.0
25	0.0	0.0	0.0	4.350497537881555	0.0
26	0.0	0.0	0.0	4.474955068164881	0.0
27	0.0	0.0	0.0	4.609714043572921	0.0
28	0.0	0.0	0.0	4.742829171354677	0.0
29	0.0	0.0	0.0	4.899871414585677	0.0
30	0.0	0.0	0.0	5.1073615149699725	0.0
31	0.0	0.0	0.0	5.2766047605827255	0.0
32	0.0	0.0	0.0	5.438724666481582	0.0
33	0.0	0.0	0.0	5.602707599690226	0.0
34	0.0	0.0	0.0	5.775202010608297	0.0
35	0.0	0.0	0.0	5.978929526425764	0.0
36	0.0	0.0	0.0	6.154784692490904	0.0
37	0.0	0.0	0.0	6.345799786665108	0.0
38	0.0	0.0	0.0	6.543061501819191	0.0
39	0.0	0.0	0.0	6.797017695106448	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACCG	75	0.0	42.0	1
TAGCACG	70	0.0	41.785717	1
CGTTTTT	9785	0.0	41.78079	1
AGGGCGA	1855	0.0	39.420483	6
CGACGGT	115	0.0	39.130436	28
TAAGGGA	4485	0.0	38.82943	4
TATAGCG	145	0.0	38.793106	1
CGGTAGT	305	0.0	38.360657	12
TCGTCCC	965	0.0	38.238342	38
ACGGGAT	1390	0.0	37.553955	5
GTAGCGT	30	1.1403339E-4	37.500004	34
AGGGATT	5120	0.0	37.441406	6
ATAGGGA	4310	0.0	37.325985	4
CGCGAGG	315	0.0	37.142857	2
CCTCGTC	995	0.0	37.085423	36
GAGGGAT	4820	0.0	36.784233	5
CTCGTCC	1035	0.0	36.739132	37
CGTAAGG	490	0.0	36.734695	2
CGTTAGG	510	0.0	36.61765	2
GGGCGAT	3980	0.0	36.57663	7
>>END_MODULE