FastQCFastQC Report
Thu 26 May 2016
SRR1547601_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547601_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2398757
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130220.5428644919014306No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC33760.1407395580294294No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAA302.1664928E-645.00000418
GTCGACA207.0348213E-445.00000433
CGTTGTC302.1664928E-645.00000436
TAACGCG406.8212103E-945.0000041
AATGCGC406.8212103E-945.00000439
CGAATCG207.0348213E-445.00000426
CGTAACG207.0348213E-445.00000416
CGTCGAC207.0348213E-445.00000432
TATCGGC253.8920778E-545.00000444
TGTCGAT351.2126657E-745.038
ACGTTAG1150.043.0434761
ATAGCGG4100.042.804882
CGGTCTA2450.042.244931
CGTTTTT77550.041.9245641
CCTCGTC13000.041.7115436
CTCGTCC13100.041.3931337
TTTCGCG603.6379788E-1241.2500041
CCCGATA603.6379788E-1241.25000416
CGTTAGG4300.040.29072
CTCCGTA451.9297659E-840.011