##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547601_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2398757
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28089339603803	31.0	31.0	33.0	30.0	34.0
2	31.685071893484835	31.0	31.0	34.0	30.0	34.0
3	31.832115966727766	31.0	31.0	34.0	30.0	34.0
4	35.60236322395307	37.0	35.0	37.0	33.0	37.0
5	35.500455027332904	37.0	35.0	37.0	33.0	37.0
6	35.55027541347456	37.0	35.0	37.0	33.0	37.0
7	35.892732360968616	37.0	35.0	37.0	35.0	37.0
8	35.923125185252196	37.0	35.0	37.0	35.0	37.0
9	37.77000671597832	39.0	38.0	39.0	35.0	39.0
10	37.106272123437265	39.0	37.0	39.0	33.0	39.0
11	36.79350722061468	39.0	35.0	39.0	32.0	39.0
12	36.03636883602633	37.0	35.0	39.0	32.0	39.0
13	35.71184450946886	37.0	35.0	39.0	31.0	39.0
14	36.60220856051697	38.0	35.0	41.0	31.0	41.0
15	36.916568456079546	38.0	35.0	41.0	32.0	41.0
16	37.05949623075618	38.0	35.0	41.0	32.0	41.0
17	36.985873100109764	38.0	35.0	41.0	32.0	41.0
18	36.91724380585445	38.0	35.0	41.0	32.0	41.0
19	36.87001559557721	38.0	35.0	41.0	32.0	41.0
20	36.70502472739006	38.0	35.0	41.0	31.0	41.0
21	36.52655396107234	38.0	35.0	40.0	31.0	41.0
22	36.467563408882185	38.0	35.0	40.0	31.0	41.0
23	36.435476373805265	38.0	35.0	40.0	31.0	41.0
24	36.357326315254106	38.0	35.0	40.0	31.0	41.0
25	36.232534183329115	38.0	35.0	40.0	31.0	41.0
26	36.1231079263135	38.0	35.0	40.0	30.0	41.0
27	36.057385554268315	38.0	35.0	40.0	30.0	41.0
28	36.102295480534295	38.0	35.0	40.0	30.0	41.0
29	36.143533088178586	38.0	35.0	40.0	30.0	41.0
30	36.15777046195175	38.0	35.0	40.0	30.0	41.0
31	36.038024276739996	38.0	35.0	40.0	30.0	41.0
32	35.91332802780774	38.0	35.0	40.0	30.0	41.0
33	35.80550176612304	38.0	35.0	40.0	30.0	41.0
34	35.69964652526288	38.0	35.0	40.0	29.0	41.0
35	35.54645593530316	38.0	34.0	40.0	29.0	41.0
36	35.43557809315408	38.0	34.0	40.0	28.0	41.0
37	35.387726226541496	38.0	34.0	40.0	28.0	41.0
38	35.31629756578094	37.0	34.0	40.0	27.0	41.0
39	35.27248070563213	37.0	34.0	40.0	27.0	41.0
40	35.162529176569365	37.0	34.0	40.0	27.0	41.0
41	35.09940398297952	37.0	34.0	40.0	27.0	41.0
42	35.08253274508422	37.0	34.0	40.0	27.0	41.0
43	35.02945358783737	37.0	34.0	40.0	27.0	41.0
44	34.92011779434099	37.0	34.0	40.0	26.0	41.0
45	34.83539349754894	37.0	34.0	40.0	26.0	41.0
46	34.8164149182264	36.0	34.0	40.0	26.0	41.0
47	34.7685105244091	36.0	34.0	40.0	26.0	41.0
48	34.6614179760601	36.0	34.0	40.0	26.0	41.0
49	34.61920819824601	36.0	34.0	40.0	26.0	41.0
50	34.5236653816956	36.0	34.0	40.0	26.0	41.0
51	34.06735696863	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	5.0
12	3.0
13	7.0
14	14.0
15	47.0
16	152.0
17	413.0
18	956.0
19	1883.0
20	3290.0
21	5487.0
22	8394.0
23	12321.0
24	17765.0
25	25791.0
26	34492.0
27	41366.0
28	45995.0
29	52545.0
30	62275.0
31	77011.0
32	94217.0
33	124524.0
34	213569.0
35	325928.0
36	162834.0
37	205207.0
38	315488.0
39	566570.0
40	205.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.05720421034727	23.986714786032934	29.041791227706682	13.914289775913108
2	29.777005340682695	25.429878891442527	31.270320420117585	13.522795347757194
3	28.835934611134018	25.470775072256174	32.0405109813124	13.652779335297405
4	25.528429932669294	28.280772083208095	31.67398781952486	14.516810164597748
5	22.629887062341037	32.411786604478905	30.6149810089142	14.34334532426586
6	21.97779933523904	40.77824473258442	26.92119293450733	10.322762997669209
7	89.17335103138834	3.3977597564071726	6.076355379056737	1.3525338331477512
8	90.11521383783351	2.425964780926121	5.697450804729283	1.7613705765110848
9	85.79776942808296	4.722320768631421	7.210109235741678	2.2698005675439408
10	45.21800249045651	30.328207484126153	13.630601182195612	10.823188843221718
11	40.02272843810356	21.74405327425829	24.21991889966345	14.01329938797469
12	38.89518613181744	20.869141809695606	24.92107370609028	15.314598352396679
13	22.81139773641098	35.28348223684183	25.848929257944842	16.05619076880234
14	16.647080133585852	36.27941471353705	29.155433418224526	17.91807173465257
15	15.600913306349915	25.11596631088518	42.72262676044301	16.560493622321896
16	18.818121218614476	22.284041276377724	41.175742269850595	17.72209523515721
17	19.024186276475692	23.30582047285323	28.537863568506523	29.132129682164553
18	22.092066849622533	25.12830603516738	33.74214228452486	19.037484830685226
19	26.757233016933352	26.979973377878625	26.175264939299815	20.087528665888208
20	27.734447465916723	26.140538620627268	27.65373899898989	18.471274914466118
21	22.506198001715056	27.936427074522346	30.583881568662434	18.973493355100164
22	22.95739001491189	23.968788835217573	29.407939195174837	23.665881954695703
23	19.280277243589076	28.29298674271716	28.684772988677054	23.74196302501671
24	20.280753740374703	25.091161797547645	36.77533822725687	17.852746234820785
25	18.65236870595896	27.271124169726235	33.14675058790865	20.929756536406146
26	17.941917418062772	34.399524420356045	27.079233119486467	20.579325042094716
27	17.559802847891635	33.55416993050984	30.39086493546449	18.495162286134025
28	15.736983779515807	29.241894864715352	36.32969075233548	18.69143060343336
29	16.754760903251142	25.321656174427005	36.68399925461395	21.2395836677079
30	19.106854091514897	30.83651241038588	31.6128728337218	18.443760664377425
31	25.98641713187288	27.858803538666066	27.01407437268552	19.14070495677553
32	26.851781985419947	27.456803669567197	27.642483169408155	18.0489311756047
33	25.832670837437888	28.170464953307068	25.73128499468683	20.26557921456821
34	18.18183334118462	28.669306645066587	29.55889237634325	23.58996763740554
35	18.817996153841342	28.26384665057778	31.16947652471676	21.74868067086412
36	26.840442779322792	26.862078985074355	27.44346342709995	18.854014808502903
37	18.98754229794848	31.375916776897366	30.03947461122573	19.597066313928423
38	19.771364919414513	31.485932088994424	26.809343339071027	21.933359652520036
39	19.484883212430436	30.9217232091454	29.09185882521656	20.501534753207597
40	23.90579787781755	27.09399076271586	27.23347967301398	21.76673168645261
41	17.86354349356771	25.588627776802735	30.13156397250743	26.416264757122125
42	21.2934448966694	25.734995249623033	27.213927880147924	25.75763197355964
43	21.14011548481151	26.77186559538961	28.070955082152967	24.017063837645914
44	19.259224673445456	29.686791950997954	28.631662148354337	22.422321227202254
45	17.58485749077543	34.967735372945235	25.528263179638454	21.919143956640877
46	21.14682729430284	32.12680567477239	26.951125103543212	19.775241927381558
47	19.56542492632643	28.21832307315831	29.15701757201751	23.05923442849776
48	21.24812976053848	26.970343390347583	31.240179809793155	20.541347039320783
49	20.310936038956843	25.775182730055608	31.75148629060801	22.16239494037954
50	19.279735296238844	30.372480413814323	29.677245339982335	20.670538949964502
51	17.810932912337517	32.37309990132389	26.946872901256775	22.869094285081815
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1029.0
1	2296.0
2	3563.0
3	12479.0
4	21395.0
5	15287.5
6	9180.0
7	9135.5
8	9091.0
9	9590.0
10	10089.0
11	10050.0
12	10011.0
13	9922.0
14	9833.0
15	9122.5
16	8412.0
17	8221.5
18	8031.0
19	7982.5
20	7934.0
21	9120.5
22	10307.0
23	10687.5
24	11068.0
25	13213.0
26	20680.0
27	26002.0
28	29261.5
29	32521.0
30	38141.0
31	43761.0
32	52823.5
33	61886.0
34	69975.0
35	78064.0
36	82807.0
37	87550.0
38	102748.5
39	117947.0
40	152429.5
41	186912.0
42	207662.5
43	228413.0
44	230810.0
45	233207.0
46	225624.5
47	218042.0
48	213087.5
49	208133.0
50	195069.0
51	182005.0
52	162221.0
53	142437.0
54	127538.0
55	112639.0
56	97558.0
57	82477.0
58	74829.5
59	67182.0
60	57903.0
61	48624.0
62	41695.5
63	34767.0
64	29616.0
65	24465.0
66	20400.0
67	16335.0
68	13729.0
69	11123.0
70	9557.0
71	7991.0
72	6362.5
73	4734.0
74	3635.0
75	2307.5
76	2079.0
77	1575.0
78	1071.0
79	708.5
80	346.0
81	230.5
82	115.0
83	72.0
84	29.0
85	33.0
86	37.0
87	28.0
88	19.0
89	11.5
90	4.0
91	2.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2398757.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.070696685277444
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.7587083350802	13.444660213123862
2	8.620842800106765	2.943275852241609
3	2.8999302228333357	1.4851148772737066
4	1.3664003547932242	0.9330162402932244
5	0.8173965747486699	0.6976764499559627
6	0.5356819828302432	0.5486678789197846
7	0.3877430243664857	0.4633330492554692
8	0.2874003558956496	0.3924899442188338
9	0.2505940918600691	0.3850034159939213
>10	2.971844140704945	12.544350735411985
>50	1.4223712974489027	17.57534336885525
>100	1.67391325875056	47.055882519419804
>500	0.005936739791187608	0.671912604741798
>1k	9.894566318646015E-4	0.30939565453388346
>5k	0.0	0.0
>10k+	2.473641579661504E-4	0.5498771957609159
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13022	0.5428644919014306	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3376	0.1407395580294294	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.168825771013904E-5	0.0	0.0	0.04235526983350127	0.0
2	4.168825771013904E-5	0.0	0.0	0.1571647315672242	0.0
3	4.168825771013904E-5	0.0	0.0	0.23741462765924184	0.0
4	4.168825771013904E-5	0.0	0.0	0.4670335511266877	0.0
5	4.168825771013904E-5	0.0	0.0	0.7882832650410192	0.0
6	4.168825771013904E-5	0.0	0.0	1.1795275636506741	0.0
7	4.168825771013904E-5	0.0	0.0	1.3567860354341852	0.0
8	4.168825771013904E-5	0.0	0.0	1.8348252865963497	0.0
9	4.168825771013904E-5	0.0	0.0	1.964684209363433	0.0
10	4.168825771013904E-5	0.0	0.0	2.2512909811206385	0.0
11	4.168825771013904E-5	0.0	0.0	2.6474961823978003	0.0
12	4.168825771013904E-5	0.0	0.0	2.9614921394705673	0.0
13	4.168825771013904E-5	0.0	0.0	3.095978458843476	0.0
14	4.168825771013904E-5	0.0	0.0	3.1464629389304544	0.0
15	4.168825771013904E-5	0.0	0.0	3.2289640009388196	0.0
16	4.168825771013904E-5	0.0	0.0	3.415935836768793	0.0
17	4.168825771013904E-5	0.0	0.0	3.62475231963888	0.0
18	4.168825771013904E-5	0.0	0.0	3.880884975009974	0.0
19	4.168825771013904E-5	0.0	0.0	4.0208324561429105	0.0
20	4.168825771013904E-5	0.0	0.0	4.187585486983467	0.0
21	4.168825771013904E-5	0.0	0.0	4.409992341867059	0.0
22	4.168825771013904E-5	0.0	0.0	4.653743584698241	0.0
23	4.168825771013904E-5	0.0	0.0	4.907583385895278	0.0
24	4.168825771013904E-5	0.0	0.0	5.097723529311223	0.0
25	4.168825771013904E-5	0.0	0.0	5.262433835523982	0.0
26	4.168825771013904E-5	0.0	0.0	5.4268940121904805	0.0
27	4.168825771013904E-5	0.0	0.0	5.592938342649964	0.0
28	4.168825771013904E-5	0.0	0.0	5.76260955153023	0.0
29	4.168825771013904E-5	0.0	0.0	5.965881496124868	0.0
30	4.168825771013904E-5	0.0	0.0	6.209841180244601	0.0
31	4.168825771013904E-5	0.0	0.0	6.423243371462804	0.0
32	4.168825771013904E-5	0.0	0.0	6.615509616021964	0.0
33	4.168825771013904E-5	0.0	0.0	6.808025990127387	0.0
34	4.168825771013904E-5	0.0	0.0	7.023054023396284	0.0
35	4.168825771013904E-5	0.0	0.0	7.272933440110857	0.0
36	4.168825771013904E-5	4.168825771013904E-5	0.0	7.484709789278364	0.0
37	4.168825771013904E-5	4.168825771013904E-5	0.0	7.705865996430651	0.0
38	4.168825771013904E-5	4.168825771013904E-5	0.0	7.961289951420674	0.0
39	4.168825771013904E-5	4.168825771013904E-5	0.0	8.387093815672033	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAA	30	2.1664928E-6	45.000004	18
GTCGACA	20	7.0348213E-4	45.000004	33
CGTTGTC	30	2.1664928E-6	45.000004	36
TAACGCG	40	6.8212103E-9	45.000004	1
AATGCGC	40	6.8212103E-9	45.000004	39
CGAATCG	20	7.0348213E-4	45.000004	26
CGTAACG	20	7.0348213E-4	45.000004	16
CGTCGAC	20	7.0348213E-4	45.000004	32
TATCGGC	25	3.8920778E-5	45.000004	44
TGTCGAT	35	1.2126657E-7	45.0	38
ACGTTAG	115	0.0	43.043476	1
ATAGCGG	410	0.0	42.80488	2
CGGTCTA	245	0.0	42.2449	31
CGTTTTT	7755	0.0	41.924564	1
CCTCGTC	1300	0.0	41.71154	36
CTCGTCC	1310	0.0	41.39313	37
TTTCGCG	60	3.6379788E-12	41.250004	1
CCCGATA	60	3.6379788E-12	41.250004	16
CGTTAGG	430	0.0	40.2907	2
CTCCGTA	45	1.9297659E-8	40.0	11
>>END_MODULE