FastQCFastQC Report
Thu 26 May 2016
SRR1547599_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547599_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3897185
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225700.5791359660883433No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT120450.041.544211
CGGTCTA2100.040.71428731
TACGGGA16500.040.6363644
TTTCGCG1000.040.51
ATACCGG850.039.7058832
ACGGGAC15000.039.4499975
ACTACGG1600.039.3750042
CGTACTC403.461064E-739.37500430
CGAGGGA29100.038.8917544
AGCGGGA31400.038.837584
GCGTAAG2150.038.7209321
GTTAGCG2050.038.4146351
GGGCGAT57100.038.380037
TAGGGAT78050.038.3696375
AGGGATC56400.038.337776
AGGGCGA32050.038.260536
TAGTACG1300.038.0769271
ACGGGAT22550.037.8159645
TAAGGGA57850.037.8046654
TAGCGCG900.037.5000041
Produced by FastQC (version 0.11.5)