##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547599_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3897185
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.369501576137647	31.0	31.0	33.0	30.0	34.0
2	31.792167680004926	31.0	31.0	34.0	30.0	34.0
3	31.932263672368645	31.0	31.0	34.0	30.0	34.0
4	35.70005016441355	37.0	35.0	37.0	33.0	37.0
5	35.60918791384037	37.0	35.0	37.0	33.0	37.0
6	35.65970206700477	37.0	35.0	37.0	33.0	37.0
7	35.98872314247335	37.0	35.0	37.0	35.0	37.0
8	36.02632438542179	37.0	35.0	37.0	35.0	37.0
9	37.87128196377642	39.0	38.0	39.0	35.0	39.0
10	37.24786788412662	39.0	37.0	39.0	34.0	39.0
11	36.9153227265321	39.0	37.0	39.0	33.0	39.0
12	36.49586945449087	39.0	35.0	39.0	32.0	39.0
13	36.35224116894631	39.0	35.0	39.0	32.0	39.0
14	37.357666880068564	39.0	35.0	41.0	32.0	41.0
15	37.55866067430723	40.0	35.0	41.0	32.0	41.0
16	37.620492227081854	40.0	35.0	41.0	33.0	41.0
17	37.52947140051088	39.0	35.0	41.0	33.0	41.0
18	37.450470788530694	39.0	36.0	41.0	32.0	41.0
19	37.4306185105403	39.0	36.0	41.0	32.0	41.0
20	37.29209673135866	39.0	35.0	41.0	32.0	41.0
21	37.13330442357753	39.0	35.0	41.0	32.0	41.0
22	37.11188203793251	39.0	35.0	41.0	32.0	41.0
23	37.03498114664816	39.0	35.0	41.0	32.0	41.0
24	36.968341764632676	39.0	35.0	41.0	32.0	41.0
25	36.86998282093357	38.0	35.0	41.0	31.0	41.0
26	36.783362863194846	38.0	35.0	41.0	31.0	41.0
27	36.70897840364263	38.0	35.0	40.0	31.0	41.0
28	36.723082173414916	38.0	35.0	40.0	31.0	41.0
29	36.748618810756994	38.0	35.0	40.0	31.0	41.0
30	36.767081624300616	38.0	35.0	41.0	31.0	41.0
31	36.69507554811999	38.0	35.0	41.0	31.0	41.0
32	36.60997258277449	39.0	35.0	41.0	31.0	41.0
33	36.50321013757366	39.0	35.0	41.0	30.0	41.0
34	36.40977834000695	39.0	35.0	41.0	30.0	41.0
35	36.265356404686976	38.0	35.0	41.0	30.0	41.0
36	36.18533172020317	38.0	35.0	41.0	30.0	41.0
37	36.159100478935436	38.0	35.0	41.0	30.0	41.0
38	36.07531179556526	38.0	35.0	41.0	30.0	41.0
39	36.01068617476461	38.0	35.0	41.0	29.0	41.0
40	35.950207393285154	38.0	35.0	41.0	29.0	41.0
41	35.87103229638829	38.0	35.0	40.0	29.0	41.0
42	35.85165087107746	38.0	35.0	40.0	29.0	41.0
43	35.82840717081688	38.0	35.0	40.0	29.0	41.0
44	35.70082533931543	38.0	35.0	40.0	29.0	41.0
45	35.6276581686525	38.0	34.0	40.0	28.0	41.0
46	35.61113239427946	38.0	34.0	40.0	28.0	41.0
47	35.5746617109529	38.0	34.0	40.0	28.0	41.0
48	35.44898227823416	38.0	34.0	40.0	28.0	41.0
49	35.37930583228664	38.0	34.0	40.0	27.0	41.0
50	35.30905178994582	38.0	34.0	40.0	27.0	41.0
51	34.89022307126811	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	0.0
9	4.0
10	2.0
11	4.0
12	8.0
13	11.0
14	16.0
15	64.0
16	150.0
17	442.0
18	984.0
19	1913.0
20	3519.0
21	5886.0
22	9509.0
23	14820.0
24	22563.0
25	33365.0
26	45525.0
27	54347.0
28	61520.0
29	70980.0
30	85491.0
31	107062.0
32	134564.0
33	177564.0
34	288520.0
35	417399.0
36	286537.0
37	370959.0
38	589198.0
39	1113936.0
40	321.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.10748270867306	24.755458106299805	29.57804158642713	13.559017598600015
2	30.287502389545274	25.944829408919517	30.835667282923445	12.932000918611767
3	28.255753832574026	25.904338644431814	32.3019050930351	13.53800242995906
4	24.896585612435643	28.467881304069476	32.12095397062239	14.514579112872497
5	22.932526939316457	32.8166869163255	30.44630932326795	13.804476821090095
6	21.82816571448366	41.2364052514828	26.658036505836908	10.277392528196634
7	91.04089233639152	2.4453291285889684	5.439541617860071	1.0742369171594368
8	91.8860664813192	1.6976869201744336	5.077536734848358	1.3387098636579993
9	86.9040858978981	4.882370223635778	6.390150839644512	1.823393038821611
10	41.87319821871427	37.39170709114399	11.515029437914803	9.220065252226927
11	29.715987308788268	24.079739606921404	29.337021465493685	16.867251618796644
12	30.427731811551155	22.97404408566696	28.499596503630187	18.098627599151694
13	23.48189783138342	28.83912362384644	29.68442606650698	17.994552478263156
14	17.810932763007145	32.63719838806728	30.54763887267348	19.00422997625209
15	17.145144508151397	27.43608527693707	38.765313938137396	16.653456276774133
16	21.985304777679275	25.388940992023727	35.83894528999778	16.786808940299217
17	21.327573620446554	24.778269443200667	29.55361369809234	24.340543238260437
18	22.186065070044148	25.781891288199045	32.9278440720674	19.104199569689403
19	24.958989629694255	26.701170203621334	29.103339974879304	19.23650019180511
20	25.991837698236036	27.556223273978524	28.916256220836324	17.53568280694912
21	23.704904950624616	26.605126520809254	31.885373673561812	17.804594855004314
22	22.92644562677933	23.92747585757412	31.23546867803299	21.91060983761356
23	19.88114498028705	27.895904351474204	30.612275270483696	21.610675397755045
24	19.670531422039243	26.901904836439634	35.15265505743248	18.274908684088643
25	19.91070477793587	28.345049054638157	31.989987644928323	19.75425852249765
26	19.439133631069605	31.648433420533024	28.33098762311771	20.581445325279656
27	18.62934400086216	31.53086137814859	31.373440059940698	18.46635456104855
28	17.515386105612126	28.908635335504982	34.43362324344367	19.14235531543922
29	18.197750427552194	26.538180763807723	34.890234874659534	20.373833933980553
30	20.4789354367319	28.436756274079883	32.161470394656654	18.922837894531565
31	23.24418779195753	27.487019476878828	29.960035256216987	19.30875747494666
32	24.340671535993287	27.24281757217068	29.8227310225201	18.59377986931593
33	23.322423749449925	27.864137832820358	28.88056379155724	19.93287462617248
34	19.21830757328687	28.101899191339392	31.123695693173403	21.55609754220033
35	19.479393459638175	28.219112000071846	31.316732462020664	20.984762078269313
36	23.403944128903298	27.51167830113274	29.489952363051792	19.59442520691217
37	20.07058941261449	30.311622363321217	30.464784196798455	19.153004027265837
38	19.83867843071345	30.34259343603139	28.79968490076812	21.01904323248704
39	19.84024366305423	29.897579919865237	29.77538915909817	20.486787257982364
40	22.42038804932278	27.30801848000544	29.13433670713605	21.13725676353573
41	18.671964507715185	26.92338187691885	30.455880334138612	23.948773281227346
42	20.07099996535961	27.51817016641499	29.517151482416153	22.893678385809242
43	20.736146731551106	26.946783383390834	30.050510817423344	22.266559067634716
44	19.68177030343697	28.97629955981048	29.91972410855528	21.422206028197277
45	18.7989279441443	31.403590027160632	27.892619929513224	21.904862099181845
46	20.742612937286786	30.194178618669632	28.87902421876303	20.184184225280553
47	19.617262203359605	28.186242120915484	30.760561790112607	21.435933885612307
48	20.5243271746145	27.074003415285645	31.884526908525	20.517142501574856
49	20.88956516049405	26.178998430918725	31.667164889529236	21.264271519057985
50	18.91203522542553	29.46644821839353	30.419392458915855	21.202124097265077
51	18.58387528433985	30.36363426421892	28.930343311903332	22.122147139537898
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1621.0
1	3067.0
2	4513.0
3	20514.0
4	36515.0
5	25475.5
6	14436.0
7	14077.5
8	13719.0
9	14412.0
10	15105.0
11	15415.0
12	15725.0
13	15968.5
14	16212.0
15	15507.5
16	14803.0
17	14455.5
18	14108.0
19	14534.0
20	14960.0
21	16067.0
22	17174.0
23	19971.5
24	22769.0
25	27422.5
26	40067.0
27	48058.0
28	58273.5
29	68489.0
30	80414.0
31	92339.0
32	106026.5
33	119714.0
34	133755.5
35	147797.0
36	163619.5
37	179442.0
38	194677.0
39	209912.0
40	240047.0
41	270182.0
42	302968.0
43	335754.0
44	352894.5
45	370035.0
46	366779.0
47	363523.0
48	345102.0
49	326681.0
50	299956.5
51	273232.0
52	246779.5
53	220327.0
54	201300.0
55	182273.0
56	163429.5
57	144586.0
58	125136.0
59	105686.0
60	90085.5
61	74485.0
62	62246.0
63	50007.0
64	40791.0
65	31575.0
66	25745.0
67	19915.0
68	16536.5
69	13158.0
70	10075.5
71	6993.0
72	5568.0
73	4143.0
74	3114.0
75	1746.5
76	1408.0
77	1091.5
78	775.0
79	577.0
80	379.0
81	365.5
82	352.0
83	224.0
84	96.0
85	58.0
86	20.0
87	17.0
88	14.0
89	9.0
90	4.0
91	4.0
92	4.0
93	3.5
94	3.0
95	2.0
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3897185.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.59882340590675
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.57370363672621	12.710333842022578
2	8.95652760250957	2.9733564000837145
3	3.1868334212568508	1.5869305555045232
4	1.5332044037261954	1.017975565704387
5	0.9783114367282492	0.8119409387115561
6	0.6473398579439066	0.64470479913694
7	0.4612642659213847	0.5359510865438992
8	0.3568271824047767	0.4738329109731331
9	0.3182976401415346	0.4755029687303569
>10	3.6333468429746656	14.90062753630294
>50	1.7085521160502497	20.5665010142677
>100	1.6404550069712365	41.956118706442794
>500	0.004080919199345535	0.481490163885885
>1k	0.0010987090152084133	0.27671159735035905
>5k	0.0	0.0
>10k+	1.5695843074405906E-4	0.5880219143392904
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22570	0.5791359660883433	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.5659546570152557E-5	0.0	0.0	0.030534860418481545	0.0
2	2.5659546570152557E-5	0.0	0.0	0.0988149138416575	0.0
3	2.5659546570152557E-5	0.0	0.0	0.16239927024249554	0.0
4	2.5659546570152557E-5	0.0	0.0	0.29983180167223267	0.0
5	2.5659546570152557E-5	0.0	0.0	0.5389531161594844	0.0
6	2.5659546570152557E-5	0.0	0.0	0.877813088164919	0.0
7	2.5659546570152557E-5	0.0	0.0	1.0434198017286838	0.0
8	2.5659546570152557E-5	0.0	0.0	1.480453198911522	0.0
9	2.5659546570152557E-5	0.0	0.0	1.6493956535294065	0.0
10	2.5659546570152557E-5	0.0	0.0	1.9333185363281447	0.0
11	2.5659546570152557E-5	0.0	0.0	2.2902684886655367	0.0
12	2.5659546570152557E-5	0.0	0.0	2.5842242541732046	0.0
13	2.5659546570152557E-5	0.0	0.0	2.714010240725036	0.0
14	2.5659546570152557E-5	0.0	0.0	2.7773123421136026	0.0
15	2.5659546570152557E-5	0.0	0.0	2.85426532227749	0.0
16	2.5659546570152557E-5	0.0	0.0	3.0040400956074707	0.0
17	2.5659546570152557E-5	0.0	0.0	3.1998224359377345	0.0
18	2.5659546570152557E-5	0.0	0.0	3.4216235564901334	0.0
19	2.5659546570152557E-5	0.0	0.0	3.5711673939009825	0.0
20	2.5659546570152557E-5	0.0	0.0	3.7341568337145916	0.0
21	2.5659546570152557E-5	0.0	0.0	3.9392279299032507	0.0
22	2.5659546570152557E-5	0.0	0.0	4.187304426143486	0.0
23	2.5659546570152557E-5	0.0	0.0	4.430479948988822	0.0
24	2.5659546570152557E-5	0.0	0.0	4.620463231794231	0.0
25	2.5659546570152557E-5	0.0	0.0	4.783093437955858	0.0
26	2.5659546570152557E-5	0.0	0.0	4.939437055207797	0.0
27	2.5659546570152557E-5	0.0	0.0	5.112254101357775	0.0
28	5.1319093140305115E-5	0.0	0.0	5.285558678892586	0.0
29	5.1319093140305115E-5	0.0	0.0	5.489013223647325	0.0
30	5.1319093140305115E-5	0.0	0.0	5.74886745176326	0.0
31	5.1319093140305115E-5	0.0	0.0	5.968975042242029	0.0
32	5.1319093140305115E-5	0.0	0.0	6.178844473639306	0.0
33	5.1319093140305115E-5	0.0	0.0	6.378988936886496	0.0
34	5.1319093140305115E-5	0.0	0.0	6.610720301961544	0.0
35	5.1319093140305115E-5	0.0	0.0	6.872986527455073	0.0
36	7.697863971045768E-5	0.0	0.0	7.109105674993617	0.0
37	7.697863971045768E-5	0.0	0.0	7.35346153698118	0.0
38	7.697863971045768E-5	0.0	0.0	7.622912435514352	0.0
39	7.697863971045768E-5	0.0	0.0	8.067181824829973	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	12045	0.0	41.54421	1
CGGTCTA	210	0.0	40.714287	31
TACGGGA	1650	0.0	40.636364	4
TTTCGCG	100	0.0	40.5	1
ATACCGG	85	0.0	39.705883	2
ACGGGAC	1500	0.0	39.449997	5
ACTACGG	160	0.0	39.375004	2
CGTACTC	40	3.461064E-7	39.375004	30
CGAGGGA	2910	0.0	38.891754	4
AGCGGGA	3140	0.0	38.83758	4
GCGTAAG	215	0.0	38.720932	1
GTTAGCG	205	0.0	38.414635	1
GGGCGAT	5710	0.0	38.38003	7
TAGGGAT	7805	0.0	38.369637	5
AGGGATC	5640	0.0	38.33777	6
AGGGCGA	3205	0.0	38.26053	6
TAGTACG	130	0.0	38.076927	1
ACGGGAT	2255	0.0	37.815964	5
TAAGGGA	5785	0.0	37.804665	4
TAGCGCG	90	0.0	37.500004	1
>>END_MODULE