FastQCFastQC Report
Thu 26 May 2016
SRR1547598_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547598_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1684184
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146120.8676011647183444No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC44480.2641041596405143No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGTG253.891581E-545.0000042
TCCGCGA253.891581E-545.00000435
TAGCGCG502.1827873E-1145.0000041
TTGTCGA253.891581E-545.00000442
CGCGAAA253.891581E-545.00000437
ATTCCGT253.891581E-545.00000439
ACTCGTC253.891581E-545.00000415
TCCCGTC253.891581E-545.00000418
CGTACCA253.891581E-545.00000423
CCGCGAA253.891581E-545.00000436
TAGTGCG253.891581E-545.0000041
TGTCACG207.0342224E-445.01
GTCGATC207.0342224E-445.016
CGGTTTG207.0342224E-445.014
CTATACG453.8562575E-1045.01
TCGATAT551.8189894E-1245.016
CCCCGAT207.0342224E-445.040
CTAGTCC351.2123746E-745.039
GTTCGAC207.0342224E-445.012
TTGCGCA207.0342224E-445.030