##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547598_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1684184
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32594063356498	31.0	31.0	33.0	30.0	34.0
2	31.729648304460795	31.0	31.0	34.0	30.0	34.0
3	31.818882616151203	31.0	31.0	34.0	30.0	34.0
4	35.59011426304964	37.0	35.0	37.0	33.0	37.0
5	35.50047916379683	37.0	35.0	37.0	33.0	37.0
6	35.55637210661068	37.0	35.0	37.0	33.0	37.0
7	35.94245996874451	37.0	35.0	37.0	35.0	37.0
8	35.979665523482	37.0	35.0	37.0	35.0	37.0
9	37.85239973779587	39.0	38.0	39.0	35.0	39.0
10	37.160899284163726	39.0	37.0	39.0	33.0	39.0
11	36.82849320501798	39.0	35.0	39.0	32.0	39.0
12	35.936130494055284	37.0	35.0	39.0	32.0	39.0
13	35.582768272350286	37.0	35.0	39.0	31.0	39.0
14	36.52705345734195	38.0	35.0	41.0	31.0	41.0
15	36.842041605905294	38.0	35.0	41.0	32.0	41.0
16	36.99689641986861	38.0	35.0	41.0	32.0	41.0
17	36.91212836602176	38.0	35.0	41.0	32.0	41.0
18	36.846520926454595	38.0	35.0	40.0	32.0	41.0
19	36.78228091467441	38.0	35.0	40.0	32.0	41.0
20	36.579803631907204	38.0	35.0	40.0	32.0	41.0
21	36.36230186250433	37.0	35.0	40.0	31.0	41.0
22	36.26788165663609	37.0	35.0	40.0	31.0	41.0
23	36.26569009086893	37.0	35.0	40.0	31.0	41.0
24	36.16138379179472	37.0	35.0	40.0	31.0	41.0
25	36.07132356084608	37.0	35.0	40.0	31.0	41.0
26	35.93913669765299	36.0	35.0	40.0	31.0	41.0
27	35.88327759912219	36.0	35.0	40.0	31.0	41.0
28	35.928411622483054	36.0	35.0	40.0	31.0	41.0
29	35.971828493798775	36.0	35.0	40.0	31.0	41.0
30	35.96471110044983	36.0	35.0	40.0	31.0	41.0
31	35.82728371721855	36.0	35.0	40.0	30.0	41.0
32	35.637636386523084	36.0	34.0	40.0	30.0	41.0
33	35.472323689098104	36.0	34.0	40.0	30.0	41.0
34	35.38446333654755	36.0	34.0	40.0	29.0	41.0
35	35.19694344560927	36.0	34.0	40.0	29.0	41.0
36	35.039714781757816	36.0	34.0	40.0	27.0	41.0
37	34.9883646917439	36.0	34.0	40.0	27.0	41.0
38	34.93680084836336	36.0	34.0	40.0	27.0	41.0
39	34.940185870427456	36.0	34.0	40.0	27.0	41.0
40	34.82232820166918	36.0	34.0	40.0	26.0	41.0
41	34.800834113137284	36.0	34.0	40.0	26.0	41.0
42	34.772436384623056	36.0	34.0	40.0	26.0	41.0
43	34.721471050669045	36.0	34.0	40.0	26.0	41.0
44	34.60981044826456	35.0	34.0	40.0	26.0	41.0
45	34.49123017437525	35.0	34.0	40.0	25.0	41.0
46	34.46073766286819	35.0	34.0	40.0	25.0	41.0
47	34.41632208832289	35.0	34.0	40.0	25.0	41.0
48	34.31543762439259	35.0	34.0	40.0	24.0	41.0
49	34.27707720771602	36.0	34.0	40.0	24.0	41.0
50	34.167612921153506	35.0	34.0	40.0	24.0	41.0
51	33.71736936106743	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	5.0
14	9.0
15	32.0
16	91.0
17	244.0
18	547.0
19	1183.0
20	2183.0
21	3730.0
22	5715.0
23	8845.0
24	13406.0
25	19521.0
26	26108.0
27	30647.0
28	32827.0
29	37095.0
30	44032.0
31	53271.0
32	67982.0
33	89857.0
34	159724.0
35	277651.0
36	116451.0
37	132062.0
38	196230.0
39	364595.0
40	139.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.808510234036184	24.940980320440048	29.932952694004932	14.317556751518836
2	30.048438887912482	26.1248770918142	30.425001068766832	13.401682951506485
3	27.41149423103414	25.282451323608345	33.70688713347235	13.599167311885163
4	24.636144269272243	28.00412543997568	33.102321361561444	14.257408929190635
5	21.898498026343916	32.59916968692257	32.316124603962514	13.186207682771004
6	20.392783686343062	41.51903829985322	27.847610474864982	10.240567538938738
7	88.48130608057076	3.148705842117013	7.084736584601208	1.2852514927110101
8	89.28745315238716	2.3064582017166773	6.494242909325822	1.9118457365703512
9	86.13067218308689	3.9634624245331866	7.940996945701895	1.964868446678035
10	48.6034186288434	24.80049685782551	14.936253996000437	11.659830517330647
11	42.777333117996605	23.44904119739886	21.737054858614023	12.036570825990509
12	40.741510428789255	22.66242880825373	23.402431088289642	13.193629674667376
13	20.62025289398308	39.9866641649606	24.48491376239176	14.908169178664563
14	14.01212694099932	43.43658412619999	27.379371850106637	15.171917082694053
15	13.05195869334942	27.33501802653392	46.744476850510395	12.868546429606267
16	15.103753509117768	21.82065617533476	46.762645886672715	16.312944428874754
17	15.343691663143694	22.71450150339868	28.678814191323514	33.262992642134115
18	19.6553345715195	26.900979940434063	34.22886097956043	19.214824508486007
19	26.871885732200283	28.511670933817207	26.805978444160495	17.810464889822015
20	30.613579038869865	25.718508191504014	26.19595008621386	17.471962683412265
21	20.64329075682942	30.9629470414159	29.613747666525747	18.780014535228933
22	21.092588458268217	26.313039430371028	27.371237346988213	25.22313476437254
23	18.40766804577172	31.629441913710142	26.246894638590557	23.715995401927582
24	18.75709542425293	25.649513354835342	39.225761555744505	16.36762966516723
25	15.836274421322136	27.739665024724143	36.04819900913439	20.375861544819333
26	15.30711608707837	37.97037615842449	28.484714259249582	18.23779349524755
27	15.90384423554671	39.066040290134566	29.107033435776614	15.923082038542107
28	13.970088778898267	32.67083644067394	38.105753290614324	15.253321489813466
29	14.098993934154464	27.159324634362992	38.341416377307944	20.400265054174604
30	16.879390850405894	34.16752563852881	31.931902927471107	17.02118058359419
31	26.90240496287817	31.443595236624976	25.67409499199612	15.979904808500734
32	28.230941512328815	30.261360991435616	26.647741576929835	14.859955919305728
33	26.883345287688282	30.897989768338853	24.792184226901572	17.426480717071293
34	19.86932544187571	30.684117649852986	26.75473701210794	22.691819896163366
35	18.513594714116746	29.312177291792345	30.737021608090327	21.43720638600058
36	27.89546747861279	28.391375289160802	27.433819582658426	16.27933764956798
37	19.47215981151703	33.820770177130285	30.478439410420716	16.228630600931965
38	18.477553521467964	34.99754183628392	25.366587023745623	21.158317618502494
39	19.259475211734582	34.05079254998266	28.475807868973934	18.213924369308817
40	23.48003543555811	28.99368477553522	26.676123273941567	20.85015651496511
41	16.347798102820118	27.105173781487057	29.06428276245351	27.482745353239313
42	20.98844306797832	27.11152700655035	26.554818238387256	25.345211687084074
43	21.484707134137363	27.752074595174875	27.038375854419705	23.724842416268054
44	18.24254357006123	33.160569153964175	28.298689454358904	20.29819782161569
45	16.920775877220066	40.07661870674463	23.041781658061115	19.96082375797419
46	21.59746203502705	36.03578943868366	24.788087287374776	17.578661238914513
47	19.906257273552058	31.28013328709927	26.655341696631723	22.158267742716948
48	22.235218954698535	26.218156685967802	31.802463388798373	19.744160970535283
49	20.27450682348247	26.048400887313978	32.581831913852646	21.09526037535091
50	18.57380191238012	34.600376205925244	28.278501636400772	18.547320245293864
51	17.265096925276573	35.53762534259914	24.72217999933499	22.475097732789294
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	935.0
1	1515.0
2	2095.0
3	10770.5
4	19446.0
5	13325.5
6	7205.0
7	7825.5
8	8446.0
9	10034.5
10	11623.0
11	12603.0
12	13583.0
13	13265.0
14	12947.0
15	12145.0
16	11343.0
17	10360.0
18	9377.0
19	9822.0
20	10267.0
21	9499.0
22	8731.0
23	9629.0
24	10527.0
25	13228.0
26	15524.0
27	15119.0
28	20270.5
29	25422.0
30	29125.5
31	32829.0
32	36614.5
33	40400.0
34	46896.5
35	53393.0
36	55504.0
37	57615.0
38	65224.0
39	72833.0
40	99369.0
41	125905.0
42	154812.0
43	183719.0
44	186319.0
45	188919.0
46	182852.5
47	176786.0
48	163622.5
49	150459.0
50	138445.0
51	126431.0
52	110298.5
53	94166.0
54	78690.0
55	63214.0
56	54265.0
57	45316.0
58	39001.5
59	32687.0
60	28042.0
61	23397.0
62	19327.0
63	15257.0
64	11928.5
65	8600.0
66	6360.5
67	4121.0
68	3269.5
69	2418.0
70	1809.0
71	1200.0
72	918.5
73	637.0
74	485.5
75	324.5
76	315.0
77	246.5
78	178.0
79	105.0
80	32.0
81	24.5
82	17.0
83	9.0
84	1.0
85	3.0
86	5.0
87	4.0
88	3.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1684184.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.449632336565223
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.60361488160682	15.093617774356543
2	9.415897068508379	3.662714722028605
3	3.407770545011456	1.9883965256354794
4	1.5862807563132009	1.234103099714415
5	0.9225988556494338	0.8972104268258649
6	0.6131089144373474	0.715484578084622
7	0.4442247773105262	0.6048006015437607
8	0.3354680305961285	0.521978388461305
9	0.2871029421547997	0.5025642000891307
>10	2.7656105010160097	12.776074770500575
>50	1.216191508355295	17.15530149253148
>100	1.3959435261057473	42.93505705244197
>500	0.005259538994632217	0.701330024745131
>1k	6.187692934861432E-4	0.3321012789848458
>5k	0.0	0.0
>10k+	3.093846467430716E-4	0.8792650640562724
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14612	0.8676011647183444	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4448	0.2641041596405143	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.9375935170978944E-5	0.0	0.0	0.05961343891166286	0.0
2	5.9375935170978944E-5	0.0	0.0	0.22236287721531614	0.0
3	5.9375935170978944E-5	0.0	0.0	0.35239617523976	0.0
4	5.9375935170978944E-5	0.0	0.0	0.651175881020126	0.0
5	5.9375935170978944E-5	0.0	0.0	1.148093082466049	0.0
6	5.9375935170978944E-5	0.0	0.0	1.7569932976444378	0.0
7	5.9375935170978944E-5	0.0	0.0	2.0624231081639537	0.0
8	5.9375935170978944E-5	0.0	0.0	2.81976316126979	0.0
9	5.9375935170978944E-5	0.0	0.0	3.0724671413574765	0.0
10	5.9375935170978944E-5	0.0	0.0	3.5142834749647305	0.0
11	5.9375935170978944E-5	0.0	0.0	4.1373745386489835	0.0
12	5.9375935170978944E-5	0.0	0.0	4.573787662155679	0.0
13	5.9375935170978944E-5	0.0	0.0	4.768303225775806	0.0
14	5.9375935170978944E-5	0.0	0.0	4.837357438379654	0.0
15	5.9375935170978944E-5	0.0	0.0	4.939543422808909	0.0
16	5.9375935170978944E-5	0.0	0.0	5.194028680951725	0.0
17	5.9375935170978944E-5	0.0	0.0	5.485148891095035	0.0
18	5.9375935170978944E-5	0.0	0.0	5.83606066795552	0.0
19	5.9375935170978944E-5	0.0	0.0	6.020363570726239	0.0
20	5.9375935170978944E-5	0.0	0.0	6.208525909283071	0.0
21	5.9375935170978944E-5	0.0	0.0	6.495192924288557	0.0
22	5.9375935170978944E-5	0.0	0.0	6.787560029070458	0.0
23	5.9375935170978944E-5	0.0	0.0	7.111633883233661	0.0
24	5.9375935170978944E-5	0.0	0.0	7.353175187509203	0.0
25	5.9375935170978944E-5	0.0	0.0	7.5548752392850185	0.0
26	5.9375935170978944E-5	0.0	0.0	7.736862480584069	0.0
27	5.9375935170978944E-5	0.0	0.0	7.910062083477815	0.0
28	5.9375935170978944E-5	0.0	0.0	8.0929993397396	0.0
29	5.9375935170978944E-5	0.0	0.0	8.303071398374524	0.0
30	5.9375935170978944E-5	0.0	0.0	8.560881708886916	0.0
31	1.7812780551293683E-4	0.0	0.0	8.802423013162457	0.0
32	1.7812780551293683E-4	0.0	0.0	9.013326334889774	0.0
33	1.7812780551293683E-4	0.0	0.0	9.23384855811479	0.0
34	1.7812780551293683E-4	0.0	0.0	9.466602223985028	0.0
35	1.7812780551293683E-4	0.0	0.0	9.750062938491281	0.0
36	1.7812780551293683E-4	0.0	0.0	10.004607572569268	0.0
37	1.7812780551293683E-4	0.0	0.0	10.244070719113825	0.0
38	1.7812780551293683E-4	0.0	0.0	10.481633835732913	0.0
39	1.7812780551293683E-4	0.0	0.0	10.727984590757304	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGTG	25	3.891581E-5	45.000004	2
TCCGCGA	25	3.891581E-5	45.000004	35
TAGCGCG	50	2.1827873E-11	45.000004	1
TTGTCGA	25	3.891581E-5	45.000004	42
CGCGAAA	25	3.891581E-5	45.000004	37
ATTCCGT	25	3.891581E-5	45.000004	39
ACTCGTC	25	3.891581E-5	45.000004	15
TCCCGTC	25	3.891581E-5	45.000004	18
CGTACCA	25	3.891581E-5	45.000004	23
CCGCGAA	25	3.891581E-5	45.000004	36
TAGTGCG	25	3.891581E-5	45.000004	1
TGTCACG	20	7.0342224E-4	45.0	1
GTCGATC	20	7.0342224E-4	45.0	16
CGGTTTG	20	7.0342224E-4	45.0	14
CTATACG	45	3.8562575E-10	45.0	1
TCGATAT	55	1.8189894E-12	45.0	16
CCCCGAT	20	7.0342224E-4	45.0	40
CTAGTCC	35	1.2123746E-7	45.0	39
GTTCGAC	20	7.0342224E-4	45.0	12
TTGCGCA	20	7.0342224E-4	45.0	30
>>END_MODULE