FastQCFastQC Report
Thu 26 May 2016
SRR1547597_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547597_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences5042923
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT382640.758766294865101No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTACG207.0355623E-445.00000435
TCCCGTA207.0355623E-445.00000425
CGTTTTT263350.043.094741
ACGTTAG2350.040.2127651
CTAAGCG2350.040.2127651
AGGGCGA42200.039.988156
GGGCGAT73650.039.256627
CGGGATC11900.039.1386576
CTATACG1800.038.751
TCACGAC3900.038.65384725
TAATCGT9900.038.63636421
ACGCGAT356.2525014E-638.57142632
AACGGTC356.2525014E-638.57142616
ACGTAAG2700.038.3333321
GACCGAT23300.038.2403459
TCGTTAG2550.037.9411771
TAAGGGA56950.037.9280054
AATCGTT9450.037.61904522
AAGGGAT71350.037.5262765
TACGGGA17150.037.2594764
Produced by FastQC (version 0.11.5)