##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547597_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	5042923
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.17817047771699	31.0	31.0	33.0	30.0	34.0
2	31.573252456958	31.0	31.0	34.0	30.0	34.0
3	31.638800156179265	31.0	31.0	34.0	30.0	34.0
4	35.46922766815992	37.0	35.0	37.0	33.0	37.0
5	35.38687840365598	37.0	35.0	37.0	33.0	37.0
6	35.43249976253851	37.0	35.0	37.0	33.0	37.0
7	35.84268687029328	37.0	35.0	37.0	35.0	37.0
8	35.87260622460426	37.0	35.0	37.0	35.0	37.0
9	37.675128690245714	39.0	37.0	39.0	35.0	39.0
10	36.96933187359791	39.0	37.0	39.0	32.0	39.0
11	36.696564472628275	39.0	35.0	39.0	32.0	39.0
12	36.13321877014581	38.0	35.0	39.0	32.0	39.0
13	35.93977322279162	38.0	35.0	39.0	31.0	39.0
14	36.934591902355045	39.0	35.0	41.0	31.0	41.0
15	37.15856557793962	39.0	35.0	41.0	32.0	41.0
16	37.2351392238192	39.0	35.0	41.0	32.0	41.0
17	37.14811945373744	39.0	35.0	41.0	32.0	41.0
18	37.02138660455454	39.0	35.0	41.0	32.0	41.0
19	36.940438114958326	38.0	35.0	41.0	32.0	41.0
20	36.769292927930884	38.0	35.0	40.0	31.0	41.0
21	36.5965732572161	38.0	35.0	40.0	31.0	41.0
22	36.54430258007112	38.0	35.0	40.0	31.0	41.0
23	36.47515657090144	38.0	35.0	40.0	31.0	41.0
24	36.380571942105796	38.0	35.0	40.0	31.0	41.0
25	36.26017153147094	38.0	35.0	40.0	30.0	41.0
26	36.12299275638355	38.0	35.0	40.0	30.0	41.0
27	36.03522738697379	38.0	35.0	40.0	30.0	41.0
28	36.060964048033256	38.0	35.0	40.0	30.0	41.0
29	36.07558889953307	38.0	35.0	40.0	30.0	41.0
30	36.04264153944052	38.0	35.0	40.0	30.0	41.0
31	35.92444441447946	38.0	35.0	40.0	30.0	41.0
32	35.75481699799898	38.0	34.0	40.0	29.0	41.0
33	35.55907833611578	38.0	34.0	40.0	28.0	41.0
34	35.37833554071716	38.0	34.0	40.0	27.0	41.0
35	35.16193842341039	38.0	34.0	40.0	26.0	41.0
36	35.04704017888039	38.0	34.0	40.0	25.0	41.0
37	35.00469132683565	38.0	34.0	40.0	25.0	41.0
38	34.921354539817486	38.0	34.0	40.0	25.0	41.0
39	34.844591519640495	38.0	34.0	40.0	24.0	41.0
40	34.756415079111854	37.0	34.0	40.0	24.0	41.0
41	34.677193365831684	37.0	34.0	40.0	23.0	41.0
42	34.63026304387356	37.0	34.0	40.0	23.0	41.0
43	34.56632353894755	37.0	33.0	40.0	23.0	41.0
44	34.426368199554105	37.0	33.0	40.0	23.0	41.0
45	34.33429877870434	37.0	33.0	40.0	23.0	41.0
46	34.29194120156108	37.0	33.0	40.0	23.0	41.0
47	34.231938699044186	36.0	33.0	40.0	23.0	41.0
48	34.09264170799356	36.0	33.0	40.0	23.0	41.0
49	34.013892934712665	36.0	33.0	40.0	23.0	41.0
50	33.90817051935951	36.0	33.0	40.0	23.0	41.0
51	33.476991617758195	35.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	3.0
11	7.0
12	10.0
13	19.0
14	48.0
15	129.0
16	397.0
17	1039.0
18	2355.0
19	4891.0
20	8381.0
21	13976.0
22	21516.0
23	31950.0
24	47330.0
25	69109.0
26	90613.0
27	102285.0
28	107474.0
29	118671.0
30	137087.0
31	163885.0
32	200816.0
33	255091.0
34	404372.0
35	583376.0
36	379176.0
37	468916.0
38	686847.0
39	1142836.0
40	313.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.73161220189164	23.477336457447397	28.961854067571522	14.829197273089436
2	32.35034522636971	24.92911353197342	29.568149265812703	13.152391975844168
3	27.878514107790263	24.88035212911242	33.27306405431929	13.968069708778025
4	24.523733556907374	27.558263332595008	33.38597476106615	14.532028349431469
5	23.00362309716012	31.13571236364307	31.798542234335127	14.062122304861685
6	21.167743390093406	40.28518777700948	28.60384741151114	9.943221421385969
7	86.57722911890585	3.1485311197493995	8.574511250717094	1.6997285106276658
8	86.82750063802283	2.6613930056040913	8.296557373570844	2.2145489828022358
9	81.85607831013878	5.082885461467486	10.1555982512523	2.905437977141432
10	41.06745234856848	30.06728439042198	16.946163960861586	11.919099300147948
11	33.99433225532097	23.615966375056686	26.93507317085746	15.454628198764883
12	32.67888484515826	21.86315357184712	28.897724593455028	16.560236989539597
13	22.271071757391496	31.365261773776837	30.065222094408345	16.298444374423326
14	17.62339024411041	32.97345606902981	31.38602354229878	18.017130144561
15	16.542846281809183	26.35931581743366	41.088670201785746	16.00916769897141
16	18.808218963486055	23.815493514376485	39.46102290278872	17.91526461934874
17	19.51834283410633	24.464402886976462	29.92655251726033	26.09070176165688
18	21.85496387710064	25.06240924162435	34.21525571578229	18.86737116549271
19	25.051324400551028	27.611942518257766	28.465792557213348	18.87094052397786
20	26.477104647443557	26.061234724385045	29.08717820993896	18.37448241823244
21	21.928195215354272	28.331366550708786	31.29145537221171	18.448982861725234
22	22.500759975910796	24.0133946126086	30.539312220313498	22.946533191167106
23	19.591494853282512	28.443622875066705	29.44106027397206	22.523821997678727
24	20.0046679277078	25.501341186450794	36.00023240489692	18.493758480944482
25	19.1865114736037	27.326393839445895	32.997450089957745	20.489644596992658
26	18.750197058333036	31.787159946721378	29.802358671746525	19.660284323199065
27	18.39649346222419	31.666773416925064	30.855220276018493	19.08151284483225
28	17.288624077742217	28.32145166602782	35.71488202377867	18.6750422324513
29	17.91363857826106	25.947372188708812	34.75898799168657	21.380001241343564
30	19.75356355827761	29.64034945605951	31.635343232486395	18.97074375317648
31	24.456272681538067	27.66506647037839	28.749199620934128	19.129461227149413
32	24.986302586813245	27.754340092045826	28.421869618076663	18.837487703064273
33	24.16840391971085	28.2325349802089	27.33367136480172	20.26538973527853
34	19.085320160549745	28.66992020302511	30.042735135951908	22.20202450047324
35	19.935581011250818	27.82963372631309	30.999600826742746	21.23518443569335
36	24.400491540322943	27.427347195267505	28.642634440383084	19.529526824026462
37	19.04583908975013	31.191632313243726	30.553272377944303	19.20925621906184
38	19.48667469243532	30.50441182623649	28.669027863403823	21.339885617924367
39	19.29896609565524	30.741337910572895	29.313693665360347	20.64600232841152
40	22.47795574114457	27.45721082792658	29.077798729030764	20.987034701898086
41	17.94510842223845	26.547678796602685	30.844611349409856	24.662601431749007
42	20.85766528658082	27.28562383363775	28.35760133557463	23.499109544206803
43	20.54142012479667	27.62134182893532	28.757944549222742	23.079293497045263
44	19.211834089078895	30.08312837614217	28.66480412252973	22.040233412249204
45	18.477101474680456	32.44181995243631	26.84157580831593	22.239502764567295
46	20.775867487962834	30.93557050147305	27.576129954790108	20.712432055774002
47	19.77702217543278	28.7704174741514	29.040379954244788	22.41218039617103
48	20.625775963662342	26.910583405695466	31.105928050061443	21.357712580580746
49	20.24387443552083	26.632332082008787	31.100336055101373	22.02345742736901
50	18.805323817159213	29.869165164726887	30.147634615876544	21.177876402237352
51	18.118499925539215	30.242282105041063	27.846052775344777	23.793165194074945
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2449.0
1	4175.5
2	5902.0
3	33915.5
4	61929.0
5	44822.0
6	27715.0
7	27678.5
8	27642.0
9	29514.5
10	31387.0
11	32162.5
12	32938.0
13	32923.5
14	32909.0
15	32516.0
16	32123.0
17	30343.0
18	28563.0
19	28076.0
20	27589.0
21	30385.5
22	33182.0
23	33305.0
24	33428.0
25	39210.0
26	50243.0
27	55494.0
28	68514.5
29	81535.0
30	90238.5
31	98942.0
32	114243.5
33	129545.0
34	148793.5
35	168042.0
36	188254.0
37	208466.0
38	223137.5
39	237809.0
40	280658.5
41	323508.0
42	370577.0
43	417646.0
44	428072.0
45	438498.0
46	443150.5
47	447803.0
48	440196.5
49	432590.0
50	404545.5
51	376501.0
52	336700.0
53	296899.0
54	262567.5
55	228236.0
56	203241.0
57	178246.0
58	159237.0
59	140228.0
60	124953.0
61	109678.0
62	94931.0
63	80184.0
64	68432.0
65	56680.0
66	49644.0
67	42608.0
68	35573.0
69	28538.0
70	22526.0
71	16514.0
72	14183.0
73	11852.0
74	9036.0
75	5242.0
76	4264.0
77	3218.5
78	2173.0
79	1391.0
80	609.0
81	628.5
82	648.0
83	386.0
84	124.0
85	80.0
86	36.0
87	30.5
88	25.0
89	18.0
90	11.0
91	13.0
92	15.0
93	9.5
94	4.0
95	3.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	5042923.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.641334460093347
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.39900985655594	15.976188493292328
2	9.69609909574165	4.002808487868241
3	3.5994706308048947	2.2289363150918104
4	1.7673446586873234	1.4592140882489826
5	1.0762631856086664	1.1107754190617005
6	0.7406162048390034	0.9172384074388127
7	0.5035255268932063	0.727540716685818
8	0.4095683625830522	0.6763230045079649
9	0.30425651397086595	0.5652234419881237
>10	2.5963548115870556	11.619279473367632
>50	0.7579440467709075	11.40529439500733
>100	1.1381780100350787	46.514337185551604
>500	0.009310897522727376	1.2603979870378317
>1k	0.0019601889521498736	0.7623443444108443
>5k	0.0	0.0
>10k+	9.800944760749368E-5	0.774098240440956
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38264	0.758766294865101	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.948930808580659E-5	0.0	0.0	0.06087739194114207	0.0
2	5.948930808580659E-5	0.0	0.0	0.19688977999465787	0.0
3	5.948930808580659E-5	0.0	0.0	0.34450258312490595	0.0
4	5.948930808580659E-5	0.0	0.0	0.5601513249359548	0.0
5	5.948930808580659E-5	0.0	0.0	0.9609902828181196	0.0
6	5.948930808580659E-5	0.0	0.0	1.5595320412387816	0.0
7	5.948930808580659E-5	0.0	0.0	1.86205500262447	0.0
8	5.948930808580659E-5	0.0	0.0	2.619314235018064	0.0
9	5.948930808580659E-5	0.0	0.0	2.911486056796822	0.0
10	5.948930808580659E-5	0.0	0.0	3.4206748744726023	0.0
11	5.948930808580659E-5	0.0	0.0	3.9559001793206043	0.0
12	5.948930808580659E-5	0.0	0.0	4.371214868836982	0.0
13	5.948930808580659E-5	0.0	0.0	4.576452981733015	0.0
14	5.948930808580659E-5	0.0	0.0	4.670406428969866	0.0
15	5.948930808580659E-5	0.0	0.0	4.791903425850444	0.0
16	5.948930808580659E-5	0.0	0.0	5.066644880360061	0.0
17	5.948930808580659E-5	0.0	0.0	5.386955144863405	0.0
18	5.948930808580659E-5	0.0	0.0	5.776411815131819	0.0
19	5.948930808580659E-5	0.0	0.0	5.994955703269711	0.0
20	5.948930808580659E-5	0.0	0.0	6.224009369169428	0.0
21	5.948930808580659E-5	0.0	0.0	6.55413536950693	0.0
22	5.948930808580659E-5	0.0	0.0	6.901890034807194	0.0
23	5.948930808580659E-5	0.0	0.0	7.319128211951679	0.0
24	5.948930808580659E-5	0.0	0.0	7.610863778804475	0.0
25	5.948930808580659E-5	0.0	0.0	7.869582779669648	0.0
26	7.931907744774211E-5	0.0	0.0	8.111168859806108	0.0
27	7.931907744774211E-5	0.0	0.0	8.367171182268697	0.0
28	7.931907744774211E-5	0.0	0.0	8.647167525659226	0.0
29	7.931907744774211E-5	0.0	0.0	8.943622577620163	0.0
30	7.931907744774211E-5	0.0	0.0	9.293261071009809	0.0
31	7.931907744774211E-5	0.0	0.0	9.614582653750613	0.0
32	7.931907744774211E-5	0.0	0.0	9.916292594592461	0.0
33	7.931907744774211E-5	0.0	0.0	10.23366805323024	0.0
34	7.931907744774211E-5	0.0	0.0	10.553958487964222	0.0
35	7.931907744774211E-5	0.0	0.0	10.902407195192154	0.0
36	7.931907744774211E-5	0.0	0.0	11.21976282406057	0.0
37	7.931907744774211E-5	0.0	0.0	11.551336397561494	0.0
38	7.931907744774211E-5	0.0	0.0	11.902263825959666	0.0
39	7.931907744774211E-5	0.0	0.0	12.328207271854042	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACTACG	20	7.0355623E-4	45.000004	35
TCCCGTA	20	7.0355623E-4	45.000004	25
CGTTTTT	26335	0.0	43.09474	1
ACGTTAG	235	0.0	40.212765	1
CTAAGCG	235	0.0	40.212765	1
AGGGCGA	4220	0.0	39.98815	6
GGGCGAT	7365	0.0	39.25662	7
CGGGATC	1190	0.0	39.138657	6
CTATACG	180	0.0	38.75	1
TCACGAC	390	0.0	38.653847	25
TAATCGT	990	0.0	38.636364	21
ACGCGAT	35	6.2525014E-6	38.571426	32
AACGGTC	35	6.2525014E-6	38.571426	16
ACGTAAG	270	0.0	38.333332	1
GACCGAT	2330	0.0	38.240345	9
TCGTTAG	255	0.0	37.941177	1
TAAGGGA	5695	0.0	37.928005	4
AATCGTT	945	0.0	37.619045	22
AAGGGAT	7135	0.0	37.526276	5
TACGGGA	1715	0.0	37.259476	4
>>END_MODULE