FastQCFastQC Report
Thu 26 May 2016
SRR1547596_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547596_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3708443
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT341110.9198199891436918No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC42440.11444155943612994No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT186150.042.48591
TATAGCG1700.042.352941
GCGTAAG3150.040.7142831
TACGGGA13150.040.551334
CGTAAGG7000.040.178572
TCGTCCC14750.039.50847238
GCGATAC1200.039.3749969
TGCGTAG2100.038.571431
GGGCGAT52050.037.9106647
GACCGAT15950.037.6645779
TAGGGAC31900.037.5235145
TATTACG1500.037.51
CGTAAAC301.14041235E-437.49999639
ACGGGAC10550.037.3222775
CGTCCCC15850.037.1924339
TAACGGG9550.036.989533
AGGGATC30750.036.951226
TGGGCGA15550.036.4630246
CTCGTCC16050.036.44859737
TAGGGCA25350.036.3017735
Produced by FastQC (version 0.11.5)