##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547596_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3708443
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.217311146483848	31.0	31.0	33.0	30.0	34.0
2	31.61477417881305	31.0	31.0	34.0	30.0	34.0
3	31.691009677107076	31.0	31.0	34.0	30.0	34.0
4	35.51147449212513	37.0	35.0	37.0	33.0	37.0
5	35.43078806927867	37.0	35.0	37.0	33.0	37.0
6	35.477283053831485	37.0	35.0	37.0	33.0	37.0
7	35.8625932770168	37.0	35.0	37.0	35.0	37.0
8	35.89182009808429	37.0	35.0	37.0	35.0	37.0
9	37.71983498195873	39.0	37.0	39.0	35.0	39.0
10	36.98123039777071	39.0	37.0	39.0	32.0	39.0
11	36.716437059973686	39.0	35.0	39.0	32.0	39.0
12	36.190317607686026	38.0	35.0	39.0	32.0	39.0
13	35.99225901544125	38.0	35.0	39.0	31.0	39.0
14	36.978546522084876	39.0	35.0	41.0	31.0	41.0
15	37.20480535901455	39.0	35.0	41.0	32.0	41.0
16	37.28563496863778	39.0	35.0	41.0	32.0	41.0
17	37.197524945104995	39.0	35.0	41.0	32.0	41.0
18	37.08379662300324	39.0	35.0	41.0	32.0	41.0
19	37.00644852839858	38.0	35.0	41.0	32.0	41.0
20	36.83283307846447	38.0	35.0	41.0	31.0	41.0
21	36.6749768029332	38.0	35.0	41.0	31.0	41.0
22	36.62387449395879	38.0	35.0	40.0	31.0	41.0
23	36.55187284798499	38.0	35.0	40.0	31.0	41.0
24	36.45108769367629	38.0	35.0	40.0	31.0	41.0
25	36.34334274518983	38.0	35.0	40.0	31.0	41.0
26	36.21171337944253	38.0	35.0	40.0	30.0	41.0
27	36.13160671473176	38.0	35.0	40.0	30.0	41.0
28	36.12290710683702	38.0	35.0	40.0	30.0	41.0
29	36.13491618989425	38.0	35.0	40.0	30.0	41.0
30	36.114500074559594	38.0	35.0	40.0	30.0	41.0
31	35.989866367097996	38.0	35.0	40.0	30.0	41.0
32	35.842056625920904	38.0	35.0	40.0	29.0	41.0
33	35.674553983976566	38.0	34.0	40.0	29.0	41.0
34	35.504187067186955	38.0	34.0	40.0	27.0	41.0
35	35.31325114070784	38.0	34.0	40.0	27.0	41.0
36	35.2006025170132	38.0	34.0	40.0	25.0	41.0
37	35.15718780091807	38.0	34.0	40.0	25.0	41.0
38	35.06468321071674	38.0	34.0	40.0	25.0	41.0
39	34.99254242279037	38.0	34.0	40.0	25.0	41.0
40	34.914113011848904	38.0	34.0	40.0	24.0	41.0
41	34.841195617675666	38.0	34.0	40.0	24.0	41.0
42	34.801088758813336	37.0	34.0	40.0	24.0	41.0
43	34.75379424734316	37.0	34.0	40.0	24.0	41.0
44	34.62370110582797	37.0	34.0	40.0	24.0	41.0
45	34.54145985255807	37.0	34.0	40.0	23.0	41.0
46	34.521157531610974	37.0	34.0	40.0	23.0	41.0
47	34.459829098087795	37.0	33.0	40.0	23.0	41.0
48	34.322518102610715	37.0	33.0	40.0	23.0	41.0
49	34.258465614814625	36.0	33.0	40.0	24.0	41.0
50	34.17406766127995	36.0	33.0	40.0	23.0	41.0
51	33.74987535200083	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	2.0
10	6.0
11	5.0
12	4.0
13	16.0
14	27.0
15	109.0
16	265.0
17	769.0
18	1714.0
19	3513.0
20	6082.0
21	9730.0
22	14651.0
23	21912.0
24	33145.0
25	47987.0
26	63538.0
27	72394.0
28	76914.0
29	84768.0
30	98225.0
31	118282.0
32	143923.0
33	184918.0
34	293901.0
35	415277.0
36	281495.0
37	345619.0
38	512755.0
39	876251.0
40	243.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.06628765765039	24.33735667502507	28.64412369288135	14.952231974443183
2	32.30328739042234	25.27705023375039	29.480134924549194	12.939527451278071
3	28.322668030761157	24.52080293535589	33.19500933410598	13.96151969977697
4	24.52339162284549	27.587831335145236	33.36251898707894	14.52625805493033
5	22.4938067000086	31.649482006329883	32.16908012338332	13.6876311702782
6	21.072024027334383	40.26352838644143	28.54421653507955	10.120231051144644
7	87.6695691426294	2.950052083852981	7.971917055216974	1.4084617183006454
8	88.21243848159457	2.378113941619165	7.718252646730717	1.6911949300555518
9	83.47961664774138	4.688625388067176	9.339094601157413	2.492663363034028
10	41.3271823242261	28.255416087020883	17.878985870889753	12.538415717863266
11	34.29654979192076	24.011775292218324	25.778338779913835	15.913336135947082
12	32.70199919481033	21.80947637593459	28.348474009173124	17.14005042008196
13	23.010357716162822	29.899286573907162	29.502273595684226	17.58808211424579
14	18.662953697818736	32.139687734178466	30.994382278492616	18.202976289510183
15	17.382605044758677	26.261236858703235	39.449790653382024	16.90636744315606
16	20.298977225752154	23.402355112374654	38.02123964154229	18.277428020330905
17	20.72988583079206	25.008797492640443	29.648049059942405	24.61326761662509
18	22.64519098716092	25.505124387782153	33.206955048250705	18.64272957680622
19	25.095356730573993	28.134341015892655	28.13431405039797	18.635988203135387
20	26.2941617277116	26.94831766323495	28.655044718228105	18.10247589082534
21	22.701629767533166	27.98759479382587	30.94616797399879	18.36460746464217
22	22.88963319646547	24.442387276816714	30.830863518732794	21.83711600798502
23	20.292613369006883	28.000106783358948	29.743722635078928	21.963557212555244
24	20.199690274328066	26.06695046950971	35.30306384647142	18.430295409690807
25	19.698159038712472	27.712438886076985	32.02314286615704	20.566259209053502
26	18.909876732634153	31.612701071581796	29.31311065048054	20.164311545303516
27	18.728776470340787	30.93818618757252	31.157280831874722	19.175756510211965
28	17.69567443803235	28.688859448561026	34.624638965733055	18.990827147673564
29	18.04843703947991	26.207980006703625	34.3016732359106	21.441909717905872
30	19.691040148116066	29.256914559560443	31.64352263200486	19.40852266031863
31	23.975857253300102	27.877009300129462	28.8534298626135	19.293703583956933
32	24.72166890525215	27.082686723242073	28.945759716409285	19.24988465509649
33	24.009375363191506	28.46806597809377	27.53470931061904	19.987849348095683
34	19.22440226262073	28.334802503368667	30.19884625434448	22.24194897966613
35	19.7818329687149	28.646065208498555	30.592839097162877	20.979262725623666
36	24.7552679116276	27.863202966851585	28.35699510549306	19.02453401602775
37	20.21058433418014	31.052519885029916	29.604257096576646	19.132638684213294
38	20.45540406041026	30.966742646442185	27.986974587448156	20.5908787056994
39	20.16075210000531	30.135585203817346	29.157519746157618	20.546142950019725
40	22.419732486113446	28.233331346875225	28.246301749817913	21.100634417193415
41	18.898308535414998	26.5046813447045	30.16624497127231	24.430765148608188
42	21.123743846137046	26.400729362700194	28.55343873426125	23.922088056901508
43	20.96971694050576	27.47039660579925	29.001497393919767	22.558389059775223
44	20.007830779656043	29.7620591714636	28.695465994758447	21.534644054121905
45	18.29751731387	32.557787729243785	26.754678445913825	22.390016510972394
46	20.74606512760207	30.93616377547127	27.263598227072656	21.054172869854007
47	19.83374154598035	28.454313575805262	29.63507865699972	22.076866221214672
48	20.875930949997073	27.40481652272935	31.35801197429757	20.361240552976007
49	20.8925686602167	26.558369644619052	30.868750038762897	21.68031165640135
50	19.635059781153437	29.38470403886483	29.84257813858808	21.137658041393653
51	18.478078266269698	31.007865025834292	27.773866282965653	22.740190424930354
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1834.0
1	3223.0
2	4612.0
3	28370.5
4	52129.0
5	36353.5
6	20578.0
7	20398.5
8	20219.0
9	21629.0
10	23039.0
11	24315.5
12	25592.0
13	25687.5
14	25783.0
15	24905.0
16	24027.0
17	22477.5
18	20928.0
19	19731.0
20	18534.0
21	19503.5
22	20473.0
23	19909.5
24	19346.0
25	23220.5
26	34171.0
27	41247.0
28	48653.5
29	56060.0
30	66673.0
31	77286.0
32	86973.5
33	96661.0
34	108438.5
35	120216.0
36	128888.0
37	137560.0
38	152790.5
39	168021.0
40	201900.0
41	235779.0
42	265910.5
43	296042.0
44	305220.5
45	314399.0
46	314460.0
47	314521.0
48	308296.5
49	302072.0
50	286016.5
51	269961.0
52	247853.5
53	225746.0
54	208509.0
55	191272.0
56	172052.0
57	152832.0
58	135110.0
59	117388.0
60	103424.0
61	89460.0
62	78830.0
63	68200.0
64	56829.5
65	45459.0
66	38430.0
67	31401.0
68	26106.5
69	20812.0
70	17000.0
71	13188.0
72	10882.0
73	8576.0
74	7021.5
75	3984.5
76	2502.0
77	1709.0
78	916.0
79	826.0
80	736.0
81	484.5
82	233.0
83	164.0
84	95.0
85	76.0
86	57.0
87	58.0
88	59.0
89	37.0
90	15.0
91	10.0
92	5.0
93	6.5
94	8.0
95	4.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3708443.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.13994169024754
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.58215358575929	15.625000494210209
2	8.713310100834686	3.5097111471971094
3	3.0540604256933666	1.8452579667587092
4	1.6487335990411638	1.32821594189764
5	0.963240755037363	0.9699806320061252
6	0.6601059223586659	0.797669687141436
7	0.4950322768651034	0.6978944833597561
8	0.3845880258630513	0.6196464332519411
9	0.32278227614750776	0.5850734598230567
>10	3.6700329996568732	17.677436658038168
>50	1.3711868608919113	19.70804155831084
>100	1.1297153190255755	34.80030155683
>500	0.003554166850337188	0.46948688495767643
>1k	0.0013669872501296876	0.42717167108446075
>5k	0.0	0.0
>10k+	1.3669872501296875E-4	0.9391114251328976
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34111	0.9198199891436918	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4244	0.11444155943612994	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.696549468334824E-5	0.0	0.0	0.0383449334397212	0.0
2	2.696549468334824E-5	0.0	0.0	0.12625244610743647	0.0
3	2.696549468334824E-5	0.0	0.0	0.21815085198828726	0.0
4	2.696549468334824E-5	0.0	0.0	0.3700744490342713	0.0
5	2.696549468334824E-5	0.0	0.0	0.6731396437804221	0.0
6	2.696549468334824E-5	0.0	0.0	1.1001382520912415	0.0
7	2.696549468334824E-5	0.0	0.0	1.329371922394385	0.0
8	2.696549468334824E-5	0.0	0.0	1.8501565212138895	0.0
9	2.696549468334824E-5	0.0	0.0	2.062995170749557	0.0
10	2.696549468334824E-5	0.0	0.0	2.422364318394539	0.0
11	2.696549468334824E-5	0.0	0.0	2.843349621390972	0.0
12	2.696549468334824E-5	0.0	0.0	3.152239362988726	0.0
13	2.696549468334824E-5	0.0	0.0	3.3082077842372124	0.0
14	2.696549468334824E-5	0.0	0.0	3.3814730332918694	0.0
15	2.696549468334824E-5	0.0	0.0	3.468490684635034	0.0
16	2.696549468334824E-5	0.0	0.0	3.6664174156108102	0.0
17	2.696549468334824E-5	0.0	0.0	3.893062398424352	0.0
18	2.696549468334824E-5	0.0	0.0	4.162285897342901	0.0
19	2.696549468334824E-5	0.0	0.0	4.327988862172076	0.0
20	2.696549468334824E-5	0.0	0.0	4.494473826347068	0.0
21	2.696549468334824E-5	0.0	0.0	4.7318241105498995	0.0
22	2.696549468334824E-5	0.0	0.0	4.988778309387524	0.0
23	2.696549468334824E-5	0.0	0.0	5.281057306260336	0.0
24	2.696549468334824E-5	0.0	0.0	5.502228293653158	0.0
25	2.696549468334824E-5	0.0	0.0	5.698968542862867	0.0
26	2.696549468334824E-5	0.0	0.0	5.8843563188108865	0.0
27	2.696549468334824E-5	0.0	0.0	6.084224565403863	0.0
28	2.696549468334824E-5	0.0	0.0	6.293422873157279	0.0
29	2.696549468334824E-5	0.0	0.0	6.519798201023987	0.0
30	2.696549468334824E-5	0.0	0.0	6.790531767644804	0.0
31	2.696549468334824E-5	0.0	0.0	7.041850178093609	0.0
32	2.696549468334824E-5	0.0	0.0	7.27782522206759	0.0
33	2.696549468334824E-5	0.0	0.0	7.512613784275503	0.0
34	2.696549468334824E-5	0.0	0.0	7.761235645255974	0.0
35	2.696549468334824E-5	0.0	0.0	8.051923677942469	0.0
36	2.696549468334824E-5	0.0	0.0	8.295098508996903	0.0
37	2.696549468334824E-5	0.0	0.0	8.56817807365517	0.0
38	2.696549468334824E-5	0.0	0.0	8.87213312972587	0.0
39	2.696549468334824E-5	0.0	0.0	9.29848456616429	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	18615	0.0	42.4859	1
TATAGCG	170	0.0	42.35294	1
GCGTAAG	315	0.0	40.714283	1
TACGGGA	1315	0.0	40.55133	4
CGTAAGG	700	0.0	40.17857	2
TCGTCCC	1475	0.0	39.508472	38
GCGATAC	120	0.0	39.374996	9
TGCGTAG	210	0.0	38.57143	1
GGGCGAT	5205	0.0	37.910664	7
GACCGAT	1595	0.0	37.664577	9
TAGGGAC	3190	0.0	37.523514	5
TATTACG	150	0.0	37.5	1
CGTAAAC	30	1.14041235E-4	37.499996	39
ACGGGAC	1055	0.0	37.322277	5
CGTCCCC	1585	0.0	37.19243	39
TAACGGG	955	0.0	36.98953	3
AGGGATC	3075	0.0	36.95122	6
TGGGCGA	1555	0.0	36.463024	6
CTCGTCC	1605	0.0	36.448597	37
TAGGGCA	2535	0.0	36.301773	5
>>END_MODULE