FastQCFastQC Report
Thu 26 May 2016
SRR1547595_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547595_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1346301
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70820.5260339255485957No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC33280.24719583510671092No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG19230.14283581457638375No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT19140.14216731622423218No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG17290.1284259612077834No Hit
GAGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCT14680.1090395089953881No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGG351.2121382E-745.0000042
TACGTAA351.2121382E-745.00000434
TCGCCTA207.0337177E-445.038
CTATGCG502.1827873E-1145.01
ATCTCGA207.0337177E-445.029
AAATCGT207.0337177E-445.015
TTTACGC207.0337177E-445.029
CTATACG253.8911636E-545.01
GACCGTA207.0337177E-445.09
CAACCCG453.8562575E-1045.01
CCTACGG253.8911636E-545.017
CAATTCG207.0337177E-445.036
CGATTCG406.8157533E-945.010
TCGATAG453.8562575E-1045.01
CCTCGAC207.0337177E-445.010
ACGATTT207.0337177E-445.034
CCGTACA207.0337177E-445.032
ACCTACG207.0337177E-445.010
TACGATC406.8157533E-945.029
ACGTCCC207.0337177E-445.034