FastQCFastQC Report
Thu 26 May 2016
SRR1547594_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547594_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1788700
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144880.809973723933583No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC69320.38754402638787944No Hit
GAGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCT18270.10214121988036003No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA600.045.0000045
CTAACCG351.2124292E-745.0000041
GCCGAAC302.1661817E-645.00000412
CTCGAAT351.2124292E-745.00000416
CTCGAAG302.1661817E-645.0000041
CGCGTCA302.1661817E-645.00000439
GCTCGAA351.2124292E-745.00000415
TAAGCGA351.2124292E-745.00000428
GGTACGA253.8916794E-545.08
CGAACTA406.8193913E-945.034
AAATCGT551.8189894E-1245.012
GTCGAAT253.8916794E-545.017
GTCGAAC253.8916794E-545.039
ATAGGCC207.0343417E-445.04
CGAAACG207.0343417E-445.041
CGTTGCC207.0343417E-445.038
GGTCCGT253.8916794E-545.08
CTAAGCG406.8193913E-945.01
CACGAAA406.8193913E-945.025
GTAGCTA551.8189894E-1245.027