##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547594_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1788700
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.287219209481748	31.0	31.0	33.0	30.0	34.0
2	31.681220998490524	31.0	31.0	34.0	30.0	34.0
3	31.76102644378599	31.0	31.0	34.0	30.0	34.0
4	35.53941969027785	37.0	35.0	37.0	33.0	37.0
5	35.44146586906692	37.0	35.0	37.0	33.0	37.0
6	35.4985643204562	37.0	35.0	37.0	33.0	37.0
7	35.899053502543744	37.0	35.0	37.0	35.0	37.0
8	35.93776597528932	37.0	35.0	37.0	35.0	37.0
9	37.772025493375075	39.0	38.0	39.0	35.0	39.0
10	37.15279644434506	39.0	37.0	39.0	33.0	39.0
11	36.79322245206016	39.0	35.0	39.0	32.0	39.0
12	35.71475820428244	37.0	35.0	39.0	32.0	39.0
13	35.29812154078381	37.0	35.0	39.0	30.0	39.0
14	36.230087773243135	38.0	35.0	40.0	31.0	41.0
15	36.58489797059317	38.0	35.0	41.0	31.0	41.0
16	36.76484877285179	38.0	35.0	41.0	32.0	41.0
17	36.690757533404145	38.0	35.0	40.0	32.0	41.0
18	36.6111164532901	38.0	35.0	40.0	32.0	41.0
19	36.50978867333818	37.0	35.0	40.0	31.0	41.0
20	36.310228098619106	37.0	35.0	40.0	31.0	41.0
21	36.096629954715716	36.0	35.0	40.0	31.0	41.0
22	36.00741655951249	36.0	35.0	40.0	31.0	41.0
23	36.018373679208366	36.0	35.0	40.0	31.0	41.0
24	35.913507016268795	36.0	35.0	40.0	31.0	41.0
25	35.83548275283726	36.0	34.0	40.0	31.0	41.0
26	35.66009615922178	36.0	34.0	40.0	30.0	41.0
27	35.61770168278638	36.0	34.0	40.0	30.0	41.0
28	35.66447978979147	36.0	34.0	40.0	30.0	41.0
29	35.72273662436406	36.0	35.0	40.0	30.0	41.0
30	35.735056186056916	36.0	35.0	40.0	30.0	41.0
31	35.544397048135515	36.0	34.0	40.0	30.0	41.0
32	35.34744563090513	36.0	34.0	40.0	29.0	41.0
33	35.15830211885727	36.0	34.0	40.0	29.0	41.0
34	35.06578185274221	36.0	34.0	40.0	28.0	41.0
35	34.89115670598759	35.0	34.0	40.0	27.0	41.0
36	34.69428914854363	35.0	34.0	40.0	25.0	41.0
37	34.64274333314698	35.0	34.0	40.0	25.0	41.0
38	34.628575501761055	35.0	34.0	40.0	26.0	41.0
39	34.60252138424554	36.0	34.0	40.0	25.0	41.0
40	34.48159948565998	36.0	34.0	40.0	24.0	41.0
41	34.45667579806563	36.0	34.0	40.0	24.0	41.0
42	34.40092637110751	35.0	34.0	40.0	24.0	41.0
43	34.31232794767149	35.0	33.0	40.0	24.0	41.0
44	34.16652373231956	35.0	33.0	40.0	23.0	41.0
45	34.07316766366635	35.0	33.0	40.0	23.0	41.0
46	34.026532118298206	35.0	33.0	40.0	23.0	41.0
47	33.98343042433052	35.0	33.0	40.0	23.0	41.0
48	33.871860569128415	35.0	33.0	40.0	23.0	41.0
49	33.86436629954716	35.0	33.0	39.0	24.0	41.0
50	33.730818471515626	35.0	33.0	39.0	24.0	41.0
51	33.27402135629228	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	7.0
14	13.0
15	31.0
16	110.0
17	302.0
18	764.0
19	1590.0
20	2667.0
21	4623.0
22	7231.0
23	10866.0
24	16011.0
25	23070.0
26	30823.0
27	36408.0
28	38892.0
29	43183.0
30	50339.0
31	61211.0
32	75286.0
33	99036.0
34	178322.0
35	309211.0
36	112522.0
37	133219.0
38	196916.0
39	355905.0
40	140.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.69676301224353	24.501146083747972	28.970704981271318	14.831385922737184
2	29.716442108794094	25.867445630905127	31.518141667132554	12.897970593168223
3	28.05456476770839	25.204953318052215	33.245261922066305	13.495219992173086
4	24.835019846816124	28.08156761894113	32.15491697881143	14.928495555431317
5	22.29306200033544	31.61340638452507	32.341029798177445	13.75250181696204
6	21.37558003018952	40.32336333650137	28.06317437244926	10.237882260859843
7	87.26773634483143	3.7760384636887125	7.436070889472801	1.520154302007044
8	87.20914630737407	3.007491474255046	7.283781517303069	2.499580701067815
9	83.16570693799966	5.0879409627103485	9.1998658243417	2.5464862749482866
10	50.70207413205121	26.318331749315142	13.830547324872814	9.149046793760832
11	46.57958293732879	18.483311902499022	22.84977916922905	12.087325990943143
12	42.89221222116621	20.058869570078826	23.773466763571307	13.275451445183654
13	20.202884776653434	42.35126069212277	24.098451389277127	13.347403141946664
14	13.75244591043775	43.92966959244144	27.300497568066195	15.017386929054622
15	12.161737574774977	24.997707832504055	49.59121149438139	13.249343098339574
16	13.601721920948176	20.9357634035892	48.70056465589535	16.761950019567283
17	14.400682059596354	21.571756023927993	28.345055067926427	35.68250684854923
18	20.432996030636772	24.788561525129982	35.705428523508694	19.07301392072455
19	26.46749035612456	27.3108402750601	26.352434729132888	19.86923463968245
20	30.36780902331302	23.720411472018785	27.50662492312853	18.405154581539666
21	19.739866942472187	29.910940906803823	30.030077710068763	20.319114440655227
22	21.022083077095097	24.474590484709566	28.02767372952423	26.475652708671106
23	17.008050539497958	31.503270531671046	26.03874322133393	25.449935707497062
24	19.116061944428914	24.4594398166266	40.05540336557276	16.369094873371722
25	15.66931290881646	25.72795885279812	36.26818359702577	22.334544641359646
26	14.797618382065187	37.90557388047185	28.108011404930956	19.188796332532007
27	15.947168334544642	36.918711913680326	30.050148152289374	17.083971599485658
28	14.048918208754962	30.063621624643595	39.85302174763795	16.034438418963493
29	13.959411863364455	24.37429418013082	40.297199083133	21.369094873371722
30	16.979258679487895	32.71336724995807	33.23503102812098	17.07234304243305
31	28.593000503158716	28.011013585285404	25.821043215743277	17.574942695812602
32	29.285570526080395	27.971543579135684	26.703080449488453	16.039805445295467
33	26.742494549113882	29.86235813719461	25.067982333538325	18.327164980153185
34	18.011852183149774	28.717560239279926	28.5087493710516	24.761838206518703
35	19.690445574998602	27.06982724883994	32.19913903952592	21.040588136635545
36	29.154357913568514	24.76105551517862	29.115167440040253	16.96941913121261
37	17.663051378095822	33.0863196735059	32.267512718734274	16.983116229664002
38	18.88097500978364	33.46413596466707	25.259406272712027	22.395482752837257
39	18.783082685749427	32.8358025381562	29.96371666573489	18.41739811035948
40	23.840610499245262	28.001174037010117	26.77967238776765	21.378543075976967
41	16.745066249231286	24.251858891932688	31.139486778107006	27.863588080729023
42	21.948621904176218	25.12886453849164	26.230055347459047	26.692458209873095
43	22.237994073908425	26.204114720187842	26.713311343433777	24.84457986246995
44	17.613294571476494	31.8273606529882	28.978196455526362	21.581148320008943
45	16.10091127634595	39.02342483367809	23.96578520713367	20.909878682842287
46	22.633141387599935	34.29960306367753	25.455246827304745	17.61200872141779
47	20.08654329960306	29.424945491138814	27.665790797786098	22.82272041147202
48	22.67289092637111	24.65242913848046	33.0343825124392	19.64029742270923
49	20.990160451724716	23.570693799966456	33.424721865041654	22.014423883267177
50	18.112987085592888	32.126013305752785	30.403086040140888	19.357913568513446
51	16.46950299099905	34.60362274277408	25.437636272152957	23.489237994073907
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	916.0
1	1658.0
2	2400.0
3	11334.5
4	20269.0
5	13994.5
6	7720.0
7	8141.5
8	8563.0
9	9899.0
10	11235.0
11	11670.5
12	12106.0
13	12094.5
14	12083.0
15	11429.5
16	10776.0
17	10167.5
18	9559.0
19	8499.0
20	7439.0
21	7297.5
22	7156.0
23	7665.0
24	8174.0
25	9576.0
26	12237.0
27	13496.0
28	18486.0
29	23476.0
30	28169.5
31	32863.0
32	37435.5
33	42008.0
34	47683.0
35	53358.0
36	53190.5
37	53023.0
38	65165.5
39	77308.0
40	107484.5
41	137661.0
42	170034.5
43	202408.0
44	205402.0
45	208396.0
46	201082.5
47	193769.0
48	178898.0
49	164027.0
50	149294.5
51	134562.0
52	114620.0
53	94678.0
54	79975.5
55	65273.0
56	56493.5
57	47714.0
58	40708.0
59	33702.0
60	31422.0
61	29142.0
62	23991.5
63	18841.0
64	15506.5
65	12172.0
66	10169.0
67	8166.0
68	6566.5
69	4967.0
70	4386.5
71	3806.0
72	2847.5
73	1889.0
74	1527.5
75	952.5
76	739.0
77	586.0
78	433.0
79	313.5
80	194.0
81	133.0
82	72.0
83	40.5
84	9.0
85	6.5
86	4.0
87	3.0
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1788700.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.154040667595215
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.03312178686853	14.166164663382213
2	9.478702625734986	3.441535058872689
3	3.423875918621328	1.8647154800235453
4	1.702746242072955	1.236468981007494
5	0.9325298072128038	0.8464592021942983
6	0.609860602334155	0.6642860505803017
7	0.40832493068875725	0.5188923178121687
8	0.3026999575848288	0.43961818720594625
9	0.23718060067960645	0.38752076432720123
>10	2.2972804889063925	9.800752277367646
>50	1.0062099646199145	13.306499662135904
>100	1.548446686461093	49.76877448558356
>500	0.016525911071772	1.8564519951695269
>1k	0.0018708578571817358	0.48935929681182344
>5k	3.1180964286362266E-4	0.3923931342393939
>10k+	3.1180964286362266E-4	0.8201084432862576
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14488	0.809973723933583	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6932	0.38754402638787944	No Hit
GAGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCT	1827	0.10214121988036003	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.5906524291384805E-5	0.0	0.0	0.10309163079331359	0.0
2	5.5906524291384805E-5	0.0	0.0	0.3550064292502935	0.0
3	5.5906524291384805E-5	0.0	0.0	0.5482752837256107	0.0
4	5.5906524291384805E-5	0.0	0.0	1.0064851568178006	0.0
5	5.5906524291384805E-5	0.0	0.0	1.6292279308995359	0.0
6	5.5906524291384805E-5	0.0	0.0	2.3097780510985633	0.0
7	5.5906524291384805E-5	0.0	0.0	2.6412478336221836	0.0
8	5.5906524291384805E-5	0.0	0.0	3.556437636272153	0.0
9	5.5906524291384805E-5	0.0	0.0	3.805053949795941	0.0
10	5.5906524291384805E-5	0.0	0.0	4.2973108961815845	0.0
11	5.5906524291384805E-5	0.0	0.0	4.986694247218651	0.0
12	5.5906524291384805E-5	0.0	0.0	5.495946776988875	0.0
13	5.5906524291384805E-5	0.0	0.0	5.695700788281992	0.0
14	5.5906524291384805E-5	0.0	0.0	5.767149326326383	0.0
15	5.5906524291384805E-5	0.0	0.0	5.887180633979986	0.0
16	5.5906524291384805E-5	0.0	0.0	6.169452675127188	0.0
17	5.5906524291384805E-5	0.0	0.0	6.501201990272265	0.0
18	5.5906524291384805E-5	0.0	0.0	6.908313300162129	0.0
19	5.5906524291384805E-5	0.0	0.0	7.112931179068597	0.0
20	5.5906524291384805E-5	0.0	0.0	7.321630234248337	0.0
21	5.5906524291384805E-5	0.0	0.0	7.6260971655392185	0.0
22	5.5906524291384805E-5	0.0	0.0	7.914463017834181	0.0
23	5.5906524291384805E-5	0.0	0.0	8.235254654218148	0.0
24	5.5906524291384805E-5	0.0	0.0	8.471795158494997	0.0
25	5.5906524291384805E-5	0.0	0.0	8.669201095767876	0.0
26	5.5906524291384805E-5	0.0	0.0	8.846145245150108	0.0
27	5.5906524291384805E-5	0.0	0.0	9.023983898921005	0.0
28	5.5906524291384805E-5	0.0	0.0	9.204170626712138	0.0
29	1.1181304858276961E-4	0.0	0.0	9.398222172527534	0.0
30	1.1181304858276961E-4	0.0	0.0	9.642477777156595	0.0
31	1.1181304858276961E-4	0.0	0.0	9.890087773243138	0.0
32	1.1181304858276961E-4	0.0	0.0	10.098451389277129	0.0
33	1.1181304858276961E-4	0.0	0.0	10.302398389892101	0.0
34	1.1181304858276961E-4	0.0	0.0	10.522334656454408	0.0
35	1.1181304858276961E-4	0.0	0.0	10.799127858221054	0.0
36	1.1181304858276961E-4	0.0	0.0	11.025493375076872	0.0
37	1.1181304858276961E-4	0.0	0.0	11.256946385643204	0.0
38	1.1181304858276961E-4	0.0	0.0	11.47962207189579	0.0
39	1.1181304858276961E-4	0.0	0.0	11.688656566221278	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGTA	60	0.0	45.000004	5
CTAACCG	35	1.2124292E-7	45.000004	1
GCCGAAC	30	2.1661817E-6	45.000004	12
CTCGAAT	35	1.2124292E-7	45.000004	16
CTCGAAG	30	2.1661817E-6	45.000004	1
CGCGTCA	30	2.1661817E-6	45.000004	39
GCTCGAA	35	1.2124292E-7	45.000004	15
TAAGCGA	35	1.2124292E-7	45.000004	28
GGTACGA	25	3.8916794E-5	45.0	8
CGAACTA	40	6.8193913E-9	45.0	34
AAATCGT	55	1.8189894E-12	45.0	12
GTCGAAT	25	3.8916794E-5	45.0	17
GTCGAAC	25	3.8916794E-5	45.0	39
ATAGGCC	20	7.0343417E-4	45.0	4
CGAAACG	20	7.0343417E-4	45.0	41
CGTTGCC	20	7.0343417E-4	45.0	38
GGTCCGT	25	3.8916794E-5	45.0	8
CTAAGCG	40	6.8193913E-9	45.0	1
CACGAAA	40	6.8193913E-9	45.0	25
GTAGCTA	55	1.8189894E-12	45.0	27
>>END_MODULE