FastQCFastQC Report
Thu 26 May 2016
SRR1547593_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547593_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences5036754
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT438110.8698260824332498No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT288850.042.593041
TACGGGA19000.039.197374
ATAGGGC33600.038.6383934
CGTTAGG9450.038.3333362
GGGCGAT72650.037.6600157
ACGGGAT21550.037.4825975
AGGGCGA39750.037.4716956
CGTAAGG8900.037.4157332
TATGGGC25650.037.28074
TAGGGCA45650.037.113915
GTACGGG18500.036.8513533
ATAGGGA72500.036.8379334
TAAGGGA64750.036.8339774
TAGGGAC51700.036.7746625
TACGGGT7900.036.740514
GTAGGGC30600.036.6176454
GACCGAT28500.036.0789459
AGGGATT68650.036.052446
GTAGGGA66850.036.0134624
CGAGGGA34250.036.04
Produced by FastQC (version 0.11.5)