##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547593_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	5036754
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.410774876041195	31.0	31.0	33.0	30.0	34.0
2	31.83154527697799	31.0	31.0	34.0	30.0	34.0
3	31.89820547122214	31.0	31.0	34.0	30.0	34.0
4	35.722582043911615	37.0	35.0	37.0	33.0	37.0
5	35.66694760157038	37.0	35.0	37.0	33.0	37.0
6	35.71928269675271	37.0	35.0	37.0	33.0	37.0
7	36.00105643436229	37.0	35.0	37.0	35.0	37.0
8	36.036836819904245	37.0	35.0	37.0	35.0	37.0
9	37.912385635669324	39.0	38.0	39.0	35.0	39.0
10	37.2369271955708	39.0	37.0	39.0	34.0	39.0
11	37.07021287916781	39.0	37.0	39.0	33.0	39.0
12	36.75696093158411	39.0	35.0	39.0	33.0	39.0
13	36.657996002981285	39.0	35.0	39.0	33.0	39.0
14	37.73895230936432	40.0	36.0	41.0	33.0	41.0
15	37.88321407001414	40.0	36.0	41.0	33.0	41.0
16	37.9133211588257	40.0	36.0	41.0	33.0	41.0
17	37.80676225203772	40.0	36.0	41.0	33.0	41.0
18	37.66930467519359	39.0	36.0	41.0	33.0	41.0
19	37.596250680497796	39.0	36.0	41.0	33.0	41.0
20	37.436691766165275	39.0	35.0	41.0	32.0	41.0
21	37.29767306483501	39.0	35.0	41.0	32.0	41.0
22	37.258772812807614	39.0	35.0	41.0	32.0	41.0
23	37.16650981961795	39.0	35.0	41.0	32.0	41.0
24	37.085053389544136	39.0	35.0	41.0	32.0	41.0
25	36.987330332194105	39.0	35.0	41.0	32.0	41.0
26	36.90513235309884	39.0	35.0	41.0	32.0	41.0
27	36.84218665434127	38.0	35.0	40.0	31.0	41.0
28	36.829083969556585	39.0	35.0	40.0	31.0	41.0
29	36.83486050738233	39.0	35.0	41.0	31.0	41.0
30	36.80987973603634	39.0	35.0	41.0	31.0	41.0
31	36.717237331821245	39.0	35.0	41.0	31.0	41.0
32	36.56168556177252	39.0	35.0	41.0	30.0	41.0
33	36.37472725489472	39.0	35.0	41.0	30.0	41.0
34	36.18334963351396	39.0	35.0	41.0	30.0	41.0
35	35.96252229114227	39.0	35.0	41.0	29.0	41.0
36	35.86442538190271	39.0	35.0	41.0	28.0	41.0
37	35.82382026201796	39.0	35.0	41.0	28.0	41.0
38	35.71471348412092	39.0	35.0	41.0	27.0	41.0
39	35.63134173318768	38.0	35.0	41.0	27.0	41.0
40	35.57201622314689	38.0	35.0	41.0	26.0	41.0
41	35.46689911796367	38.0	35.0	41.0	26.0	41.0
42	35.435052615235925	38.0	35.0	40.0	26.0	41.0
43	35.39767060293197	38.0	35.0	40.0	26.0	41.0
44	35.27502891743373	38.0	34.0	40.0	26.0	41.0
45	35.190796890219374	38.0	34.0	40.0	25.0	41.0
46	35.172894487203465	38.0	34.0	40.0	25.0	41.0
47	35.134580922554484	38.0	34.0	40.0	25.0	41.0
48	34.99602561490992	38.0	34.0	40.0	24.0	41.0
49	34.91484972265868	38.0	34.0	40.0	24.0	41.0
50	34.84239393069425	38.0	34.0	40.0	24.0	41.0
51	34.450776829680386	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	6.0
11	10.0
12	5.0
13	12.0
14	27.0
15	72.0
16	233.0
17	614.0
18	1392.0
19	2845.0
20	5227.0
21	8607.0
22	14085.0
23	21748.0
24	34625.0
25	54979.0
26	76276.0
27	88107.0
28	90914.0
29	95741.0
30	109013.0
31	131177.0
32	164004.0
33	213138.0
34	336595.0
35	458108.0
36	381615.0
37	502519.0
38	795732.0
39	1448932.0
40	395.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.41463728425093	23.007377370425477	29.308558647096923	16.269426698226674
2	32.5623804537605	24.956847207546765	29.6207041280952	12.86006821059754
3	27.36379422143706	24.71137164928047	34.38879484683985	13.536039282442621
4	24.5202564985306	27.30951323014783	34.11895836088084	14.051271910440732
5	22.54162105197117	31.612324127801354	32.444725313167964	13.401329507059506
6	20.16961717804761	40.3891673089454	29.06421477006818	10.377000742938804
7	88.112006264352	2.5710407933363433	8.062851590528345	1.254101351783311
8	89.1708231134576	1.846129471481037	7.724300213987025	1.2587472010743428
9	85.19836386688728	3.6344240755057724	9.045885504831087	2.1213265527758556
10	34.68485854183071	31.537196376872885	18.83796588040631	14.939979200890097
11	28.1925422603526	28.310912146989907	27.65163833691302	15.844907255744472
12	27.991480227146294	23.311879039556032	30.95118403638534	17.74545669691234
13	22.26652324096035	25.873906091105503	32.42368001296072	19.43589065497342
14	17.96067467261653	29.66035267952336	33.09822159271626	19.28075105514385
15	18.24631498778777	26.91666100826048	37.9190843944334	16.917939609518353
16	22.11819358261293	25.429294343142427	35.25091755523498	17.201594519009664
17	22.039174436551797	25.569523546315743	31.17587239718279	21.215429619949674
18	22.4663344685883	26.281589293421913	33.06365567982871	18.18842055816107
19	24.21041408812104	27.270956651843626	30.50081461195047	18.017814648084858
20	25.534977487484994	27.4839509731863	30.15630701836937	16.82476452095933
21	23.872458333283696	26.412109862820383	32.27870569021239	17.436726113683534
22	23.34503531441083	23.550624072567373	32.797948837683954	20.306391775337847
23	21.173160333024008	26.257168803558802	32.465452948466414	20.10421791495078
24	20.471319425169465	26.12676735850113	35.04121900732098	18.36069420900842
25	20.534931823154356	27.719042859746573	32.27036698635669	19.47565833074238
26	20.034510321528508	29.685309228920055	30.90091356456956	19.379266884981874
27	18.897944985996933	29.76651232122911	32.10587612577466	19.229666566999303
28	18.067251249515063	29.12516672444197	33.97309060557653	18.834491420466435
29	19.211678791539157	26.54304736741163	34.33806773171769	19.907206109331526
30	20.545116954292386	27.590150323005652	33.42378841611085	18.44094430659111
31	22.57418170512199	27.125684518243297	31.437290763058908	18.862843013575805
32	23.20828057117739	27.28795172446381	30.950151625431776	18.553616078927025
33	22.803575477380868	27.491594785054023	30.591249840671193	19.113579896893913
34	20.16461395573419	27.94535925320157	31.681237558951658	20.208789232112586
35	20.787217322902805	28.24408339180353	31.30825924792039	19.660440037373274
36	22.838359784893207	28.453226026127144	29.704091166652173	19.004323022327473
37	21.080402179657774	29.74784950783779	30.337773097514788	18.833975214989653
38	21.401184175363735	29.167436011367638	29.3560694050176	20.075310408251028
39	20.92091454138916	28.97314421152989	29.551056096843325	20.554885150237634
40	22.114401457764266	27.77463421878456	29.767624148409872	20.343340175041305
41	20.259933282427532	26.984442758173223	30.639495198693446	22.116128760705802
42	20.88333081186812	27.913136119016336	29.95224702258637	21.251286046529174
43	20.94193998753959	27.323669172645715	30.383814655232317	21.350576184582373
44	20.66144187307937	28.881934674593996	29.767207213217084	20.689416239109555
45	19.785798552003932	29.69061026208546	29.024506656469622	21.499084529440985
46	20.919802714208398	29.005744572794306	29.78017191230701	20.294280800690288
47	20.400480150509633	28.51574645098808	30.47220491610271	20.611568482399576
48	20.74955417715457	27.877061297812045	31.157805205495443	20.21557931953794
49	21.166251121257858	26.832658493942724	30.987794917123214	21.013295467676205
50	20.055059270315763	28.86464179112182	30.741981839891324	20.338317098671087
51	19.920825992295832	29.223484013712003	29.684872439670468	21.17081755432169
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2220.0
1	4050.0
2	5880.0
3	41264.0
4	76648.0
5	54869.5
6	33091.0
7	32099.5
8	31108.0
9	31783.5
10	32459.0
11	32486.0
12	32513.0
13	31650.5
14	30788.0
15	29180.5
16	27573.0
17	27038.5
18	26504.0
19	25295.0
20	24086.0
21	25682.5
22	27279.0
23	31538.0
24	35797.0
25	43162.0
26	56913.5
27	63300.0
28	77294.5
29	91289.0
30	105486.0
31	119683.0
32	138061.5
33	156440.0
34	179557.0
35	202674.0
36	216496.0
37	230318.0
38	249070.5
39	267823.0
40	296039.0
41	324255.0
42	340994.0
43	357733.0
44	371890.0
45	386047.0
46	390865.5
47	395684.0
48	389701.5
49	383719.0
50	374555.5
51	365392.0
52	329327.5
53	293263.0
54	266826.0
55	240389.0
56	217627.5
57	194866.0
58	175948.5
59	157031.0
60	139730.5
61	122430.0
62	104406.5
63	86383.0
64	73333.5
65	60284.0
66	50076.5
67	39869.0
68	32188.5
69	24508.0
70	20089.5
71	15671.0
72	12292.5
73	8914.0
74	7407.5
75	4644.0
76	3387.0
77	2542.5
78	1698.0
79	1237.0
80	776.0
81	517.5
82	259.0
83	243.5
84	228.0
85	128.0
86	28.0
87	23.0
88	18.0
89	12.5
90	7.0
91	9.0
92	11.0
93	6.0
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	5036754.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.6379611676795
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.03095948072192	13.763070331977456
2	7.589693452911357	2.677334367940837
3	2.462989165124627	1.3032632175265058
4	1.3452023592387823	0.9490650789969796
5	0.8307411796560441	0.7326290333582783
6	0.603743382678729	0.6389281403358532
7	0.4443464671789179	0.548615601316813
8	0.39405270736248954	0.5560229080382852
9	0.30347598814095034	0.48174279247379215
>10	4.652783986015612	20.934129920339416
>50	2.1426534376670805	27.1250593419662
>100	1.196042072229408	28.81691888111058
>500	0.0018296943857685035	0.22253615147644193
>1k	0.0013722707892811947	0.36701421301241
>5k	0.0	0.0
>10k+	1.1435589910676624E-4	0.8836700201300668
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43811	0.8698260824332498	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9854056799279855E-5	0.0	0.0	0.02307041400076319	0.0
2	1.9854056799279855E-5	0.0	0.0	0.07492921036048217	0.0
3	1.9854056799279855E-5	0.0	0.0	0.14580819313391125	0.0
4	1.9854056799279855E-5	0.0	0.0	0.23668021110421514	0.0
5	1.9854056799279855E-5	0.0	0.0	0.41572409532012083	0.0
6	1.9854056799279855E-5	0.0	0.0	0.7512576552279504	0.0
7	1.9854056799279855E-5	0.0	0.0	0.9262711659136023	0.0
8	1.9854056799279855E-5	0.0	0.0	1.2981575038209132	0.0
9	1.9854056799279855E-5	0.0	0.0	1.5083126950412904	0.0
10	1.9854056799279855E-5	0.0	0.0	1.805329384758517	0.0
11	1.9854056799279855E-5	0.0	0.0	2.0828890988124495	0.0
12	1.9854056799279855E-5	0.0	0.0	2.2817473317140364	0.0
13	1.9854056799279855E-5	0.0	0.0	2.3906468332580864	0.0
14	1.9854056799279855E-5	0.0	0.0	2.460334572623559	0.0
15	1.9854056799279855E-5	0.0	0.0	2.5260515006291753	0.0
16	1.9854056799279855E-5	0.0	0.0	2.6408476570426114	0.0
17	1.9854056799279855E-5	0.0	0.0	2.7704152317147117	0.0
18	1.9854056799279855E-5	0.0	0.0	2.9265078262706496	0.0
19	1.9854056799279855E-5	0.0	0.0	3.034771997997123	0.0
20	1.9854056799279855E-5	0.0	0.0	3.1477614352418244	0.0
21	1.9854056799279855E-5	0.0	0.0	3.2967065693500217	0.0
22	1.9854056799279855E-5	0.0	0.0	3.4649498466671194	0.0
23	1.9854056799279855E-5	0.0	0.0	3.641492119726316	0.0
24	1.9854056799279855E-5	0.0	0.0	3.782773587909991	0.0
25	1.9854056799279855E-5	0.0	0.0	3.9096211568005903	0.0
26	1.9854056799279855E-5	0.0	0.0	4.029619076095438	0.0
27	1.9854056799279855E-5	0.0	0.0	4.163217024297792	0.0
28	1.9854056799279855E-5	0.0	0.0	4.2954053344673975	0.0
29	1.9854056799279855E-5	0.0	0.0	4.45191486421612	0.0
30	1.9854056799279855E-5	0.0	0.0	4.644876442248321	0.0
31	1.9854056799279855E-5	0.0	0.0	4.808751827069577	0.0
32	1.9854056799279855E-5	0.0	0.0	4.972547795663636	0.0
33	1.9854056799279855E-5	0.0	0.0	5.1340605477257775	0.0
34	1.9854056799279855E-5	0.0	0.0	5.313878740156855	0.0
35	1.9854056799279855E-5	0.0	0.0	5.499772273968512	0.0
36	1.9854056799279855E-5	0.0	0.0	5.673971768325393	0.0
37	1.9854056799279855E-5	0.0	0.0	5.863121367452133	0.0
38	1.9854056799279855E-5	0.0	0.0	6.079709273075477	0.0
39	1.9854056799279855E-5	0.0	0.0	6.422152044749455	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	28885	0.0	42.59304	1
TACGGGA	1900	0.0	39.19737	4
ATAGGGC	3360	0.0	38.638393	4
CGTTAGG	945	0.0	38.333336	2
GGGCGAT	7265	0.0	37.660015	7
ACGGGAT	2155	0.0	37.482597	5
AGGGCGA	3975	0.0	37.471695	6
CGTAAGG	890	0.0	37.415733	2
TATGGGC	2565	0.0	37.2807	4
TAGGGCA	4565	0.0	37.11391	5
GTACGGG	1850	0.0	36.851353	3
ATAGGGA	7250	0.0	36.837933	4
TAAGGGA	6475	0.0	36.833977	4
TAGGGAC	5170	0.0	36.774662	5
TACGGGT	790	0.0	36.74051	4
GTAGGGC	3060	0.0	36.617645	4
GACCGAT	2850	0.0	36.078945	9
AGGGATT	6865	0.0	36.05244	6
GTAGGGA	6685	0.0	36.013462	4
CGAGGGA	3425	0.0	36.0	4
>>END_MODULE