FastQCFastQC Report
Thu 26 May 2016
SRR1547592_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547592_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3018922
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148630.4923280561736938No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC43360.14362742727370897No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCG207.0351124E-445.024
GTCGTAA207.0351124E-445.08
TATCTCG603.6379788E-1241.24999634
CGTTTTT86050.040.398021
TAACGGG8050.040.2484473
CGTAAGG5350.039.5327152
CGTTAGG6000.039.3752
GGCGATA8850.038.8983048
GGGCGAT43100.038.5788847
ACGCGAA356.2516683E-638.57142621
ATCGATT356.2516683E-638.57142623
CGTGAGT3000.038.2532
ATAGGGA40400.038.094064
GTAGGGC14400.037.81254
CACGACC2750.037.63636427
AGGGCGA23600.037.3728836
TAGGGAT46650.037.331195
TAAGGGA40700.036.818184
TATGGGA32150.036.6718524
AGGGATT46950.036.5654956