##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547592_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3018922
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.301470524909224	31.0	31.0	33.0	30.0	34.0
2	31.714060184396946	31.0	31.0	34.0	30.0	34.0
3	31.870857544514234	31.0	31.0	34.0	30.0	34.0
4	35.634512253049266	37.0	35.0	37.0	33.0	37.0
5	35.53535798540009	37.0	35.0	37.0	33.0	37.0
6	35.59082049817783	37.0	35.0	37.0	33.0	37.0
7	35.89265605404843	37.0	35.0	37.0	35.0	37.0
8	35.910465060044615	37.0	35.0	37.0	35.0	37.0
9	37.73903664950602	39.0	37.0	39.0	35.0	39.0
10	37.10569501298808	39.0	37.0	39.0	33.0	39.0
11	36.84183427064363	39.0	37.0	39.0	32.0	39.0
12	36.28764605379006	38.0	35.0	39.0	32.0	39.0
13	36.091074231132836	38.0	35.0	39.0	32.0	39.0
14	37.10887064985449	39.0	35.0	41.0	32.0	41.0
15	37.30603274943837	39.0	35.0	41.0	32.0	41.0
16	37.38761882552779	39.0	35.0	41.0	32.0	41.0
17	37.28288872650569	39.0	35.0	41.0	32.0	41.0
18	37.215201320206354	39.0	35.0	41.0	32.0	41.0
19	37.18703133105128	39.0	35.0	41.0	32.0	41.0
20	37.05182214048591	39.0	35.0	41.0	32.0	41.0
21	36.873005662286076	39.0	35.0	41.0	31.0	41.0
22	36.81397366344675	38.0	35.0	41.0	31.0	41.0
23	36.744312042510536	38.0	35.0	41.0	31.0	41.0
24	36.64612268882733	38.0	35.0	40.0	31.0	41.0
25	36.53437717171891	38.0	35.0	40.0	31.0	41.0
26	36.42579669166676	38.0	35.0	40.0	31.0	41.0
27	36.334958637553406	38.0	35.0	40.0	30.0	41.0
28	36.345527973230176	38.0	35.0	40.0	30.0	41.0
29	36.36738146927943	38.0	35.0	40.0	30.0	41.0
30	36.40101897299765	38.0	35.0	40.0	30.0	41.0
31	36.34796294836369	38.0	35.0	40.0	30.0	41.0
32	36.24589803910138	38.0	35.0	40.0	30.0	41.0
33	36.13511213605386	38.0	35.0	41.0	30.0	41.0
34	36.04127168572093	38.0	35.0	41.0	30.0	41.0
35	35.88533059151578	38.0	35.0	41.0	29.0	41.0
36	35.79744458452388	38.0	35.0	40.0	29.0	41.0
37	35.75912130223967	38.0	35.0	40.0	29.0	41.0
38	35.673812705329915	38.0	34.0	40.0	29.0	41.0
39	35.604402167396174	38.0	35.0	40.0	28.0	41.0
40	35.50788725246959	38.0	34.0	40.0	28.0	41.0
41	35.441707669161374	38.0	34.0	40.0	27.0	41.0
42	35.40943555348565	38.0	34.0	40.0	27.0	41.0
43	35.358828416235994	38.0	34.0	40.0	27.0	41.0
44	35.238835584357595	38.0	34.0	40.0	27.0	41.0
45	35.154840701415935	37.0	34.0	40.0	27.0	41.0
46	35.12285378688154	37.0	34.0	40.0	27.0	41.0
47	35.07503009352345	37.0	34.0	40.0	27.0	41.0
48	34.94222010373239	37.0	34.0	40.0	26.0	41.0
49	34.86861734089188	37.0	34.0	40.0	26.0	41.0
50	34.79269553834117	37.0	34.0	40.0	26.0	41.0
51	34.358579983186054	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	2.0
11	5.0
12	4.0
13	12.0
14	22.0
15	75.0
16	202.0
17	455.0
18	1043.0
19	2079.0
20	3750.0
21	6170.0
22	9658.0
23	13935.0
24	19938.0
25	28924.0
26	38960.0
27	47243.0
28	53613.0
29	61655.0
30	74009.0
31	91004.0
32	112944.0
33	146644.0
34	241020.0
35	352910.0
36	225049.0
37	288226.0
38	436236.0
39	762905.0
40	227.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.967363847095086	24.101517031576172	29.543260806340804	13.387858314987932
2	30.456732568777863	25.916734516492973	29.95009476892745	13.676438145801715
3	29.23977499253045	25.68430055496631	31.440494322145458	13.63543013035779
4	26.485613076455767	28.020896200696804	30.960091052369027	14.533399670478403
5	22.97452534381478	32.829698813020016	29.48035755809524	14.715418285069969
6	22.357815140636294	41.48464253134066	25.901364791803168	10.256177536219882
7	89.1519886900026	3.331056582448967	6.008932989987817	1.508021737560626
8	89.55998200682231	3.038170578769508	5.601535912487968	1.800311501920222
9	84.29840187987632	5.659536748547992	7.237417859752587	2.8046435118230946
10	41.921752201613685	32.50935267622019	14.184798414798394	11.384096707367728
11	35.76286502268028	23.60511467338341	24.437994754418963	16.19402554951734
12	34.73614091387588	21.168980185642425	26.852366506984943	17.24251239349675
13	23.90942859736025	30.593933861159712	27.43466045164466	18.06197708983538
14	18.889325394958863	32.54201334118603	29.163125115521364	19.405536148333745
15	17.72470438123277	26.315353626228173	38.398044070035596	17.561897922503462
16	20.722562557098197	23.780939023929733	37.07363091858617	18.4228675003859
17	20.643395225183028	24.311327023354696	29.1351018674878	25.91017588397448
18	22.63861073588519	25.512186137965802	32.23488384264317	19.614319283505836
19	25.815340707709574	27.011131788101846	27.49534436464407	19.678183139544515
20	28.04544138603117	25.832565399172285	27.426876216079783	18.69511699871676
21	24.217551828102877	27.30630337584078	29.140534270179884	19.335610525876458
22	23.712835243838697	24.30264842881002	28.917043898451166	23.067472428900118
23	21.736533769338855	27.843581251850825	28.158925603245134	22.260959375565186
24	21.711027976211376	25.047185717285842	34.38439283956326	18.857393466939524
25	20.318179800604323	27.438834126883705	31.16231555502262	21.080670517489356
26	19.960204337839798	31.715261275382407	27.567423073534197	20.757111313243602
27	19.495667658853062	31.996487487917875	28.737244619105763	19.770600234123307
28	18.39298928557942	28.290959488188168	34.34788974342497	18.96816148280744
29	18.636884291810123	25.79102739322182	33.6590677069497	21.913020608018357
30	20.049706484632594	29.001014269331897	31.2146852419506	19.7345940040849
31	25.487640952631434	27.499948657169675	27.081686774285657	19.93072361591323
32	26.135719968916053	27.10566884470682	27.746857984406354	19.01175320197077
33	24.940988869536874	27.612836635063776	26.444306941351915	21.001867554047436
34	20.175546105530383	27.975052021880657	29.130398201742214	22.719003670846746
35	20.805870439845748	26.99258212037277	29.986067874559197	22.21547956522229
36	25.29008699131677	27.29692916875626	27.73711940884859	19.675864431078377
37	20.664528596631513	30.6978451248492	28.858711818324558	19.778914460194734
38	21.11531864685474	30.5155283905977	26.76584555679146	21.603307405756095
39	20.967915037221896	29.468863388984545	28.292383837674507	21.270837736119052
40	23.192616437258067	27.379210194897386	27.988334908950947	21.439838458893608
41	19.25425698312179	26.0847083826611	29.469029011017838	25.192005623199275
42	21.477765904518233	25.835712217804897	27.7990951737077	24.887426703969165
43	21.34215458365602	26.136481830269215	28.760232957327155	23.761130628747612
44	19.94354276129029	28.68583553997089	28.854637516305488	22.515984182433332
45	19.253693868208586	31.90499787672553	26.792378206525374	22.048930048540505
46	21.111840584155537	30.814973026795656	27.01540483656086	21.057781552487942
47	20.864103146752385	27.89018729202013	28.80074410667119	22.444965454556296
48	22.0185880920408	26.480048176137043	30.6426598633552	20.858703868466957
49	20.895505084265178	26.09775939888477	30.78039114624359	22.226344370606462
50	19.914625154276923	29.426331650834303	29.646840825963704	21.012202368925067
51	19.50775806728362	30.091966602648228	27.48828886602569	22.911986464042464
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1641.0
1	3180.0
2	4719.0
3	15063.0
4	25407.0
5	18137.0
6	10867.0
7	10812.5
8	10758.0
9	11225.5
10	11693.0
11	11794.0
12	11895.0
13	11797.0
14	11699.0
15	11471.0
16	11243.0
17	11139.5
18	11036.0
19	11760.5
20	12485.0
21	12258.0
22	12031.0
23	14025.0
24	16019.0
25	17986.0
26	24990.0
27	30027.0
28	37486.0
29	44945.0
30	55925.0
31	66905.0
32	74900.5
33	82896.0
34	93104.5
35	103313.0
36	105389.0
37	107465.0
38	121850.5
39	136236.0
40	163219.5
41	190203.0
42	220530.0
43	250857.0
44	255709.0
45	260561.0
46	260886.0
47	261211.0
48	251867.0
49	242523.0
50	231834.0
51	221145.0
52	202989.5
53	184834.0
54	168444.0
55	152054.0
56	136915.5
57	121777.0
58	110155.0
59	98533.0
60	90707.5
61	82882.0
62	74369.0
63	65856.0
64	56272.5
65	46689.0
66	40107.5
67	33526.0
68	28135.0
69	22744.0
70	19097.5
71	15451.0
72	13032.5
73	10614.0
74	8766.0
75	5282.5
76	3647.0
77	2748.5
78	1850.0
79	1449.0
80	1048.0
81	693.0
82	338.0
83	319.0
84	300.0
85	189.0
86	78.0
87	52.5
88	27.0
89	17.5
90	8.0
91	9.0
92	10.0
93	5.5
94	1.0
95	2.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3018922.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.031559249192092
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.97459537272978	13.280289410224267
2	8.142071094846932	2.7734433252603963
3	2.807075347860519	1.4342661031209873
4	1.3793652363579751	0.9397096299722681
5	0.7788538547257178	0.6632547786611355
6	0.5572920075242643	0.5694931107150427
7	0.40710340764433867	0.48535240654897877
8	0.3237856305361564	0.4411659320410854
9	0.25707803311937966	0.39405957774646333
>10	4.06540545154177	17.96275889611735
>50	1.80711942380074	21.97878872005605
>100	1.4931468046360856	37.59435932529284
>500	0.006318519712320475	0.7440179714874171
>1k	5.923612230300446E-4	0.23920628417962386
>5k	0.0	0.0
>10k+	1.9745374101001485E-4	0.4998345285760929
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14863	0.4923280561736938	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4336	0.14362742727370897	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.312440665906572E-5	0.0	0.0	0.028983855826682503	0.0
2	3.312440665906572E-5	0.0	0.0	0.10298378030303532	0.0
3	3.312440665906572E-5	0.0	0.0	0.16595327736191925	0.0
4	3.312440665906572E-5	0.0	0.0	0.3239898215323218	0.0
5	3.312440665906572E-5	0.0	0.0	0.5737809721483363	0.0
6	3.312440665906572E-5	0.0	0.0	0.9277483817071127	0.0
7	3.312440665906572E-5	0.0	0.0	1.0863149163840602	0.0
8	3.312440665906572E-5	0.0	0.0	1.5174290690518006	0.0
9	3.312440665906572E-5	0.0	0.0	1.6622489749652358	0.0
10	3.312440665906572E-5	0.0	0.0	1.9413221010678645	0.0
11	3.312440665906572E-5	0.0	0.0	2.2693862246192515	0.0
12	3.312440665906572E-5	0.0	0.0	2.5590591608527813	0.0
13	3.312440665906572E-5	0.0	0.0	2.6837063031108457	0.0
14	3.312440665906572E-5	0.0	0.0	2.735943492412192	0.0
15	3.312440665906572E-5	0.0	0.0	2.811964005694748	0.0
16	3.312440665906572E-5	0.0	0.0	2.965694376999472	0.0
17	3.312440665906572E-5	0.0	0.0	3.1373781767134097	0.0
18	3.312440665906572E-5	0.0	0.0	3.340132669873551	0.0
19	3.312440665906572E-5	0.0	0.0	3.469185358217271	0.0
20	3.312440665906572E-5	0.0	0.0	3.6090697275385053	0.0
21	3.312440665906572E-5	0.0	0.0	3.8030793773406533	0.0
22	3.312440665906572E-5	0.0	0.0	4.009775674893223	0.0
23	3.312440665906572E-5	0.0	0.0	4.225614308683696	0.0
24	3.312440665906572E-5	0.0	0.0	4.394615031458249	0.0
25	3.312440665906572E-5	0.0	0.0	4.540859286858024	0.0
26	3.312440665906572E-5	0.0	0.0	4.680644282959282	0.0
27	3.312440665906572E-5	0.0	0.0	4.821721130920243	0.0
28	3.312440665906572E-5	0.0	0.0	4.969224113773062	0.0
29	3.312440665906572E-5	0.0	0.0	5.136667989434639	0.0
30	3.312440665906572E-5	0.0	0.0	5.350949776112135	0.0
31	3.312440665906572E-5	0.0	0.0	5.546185028960669	0.0
32	3.312440665906572E-5	0.0	0.0	5.721578762220422	0.0
33	3.312440665906572E-5	0.0	0.0	5.89925807953965	0.0
34	3.312440665906572E-5	0.0	0.0	6.089160302916074	0.0
35	6.624881331813144E-5	0.0	0.0	6.306323912972909	0.0
36	6.624881331813144E-5	0.0	0.0	6.5089127840997545	0.0
37	6.624881331813144E-5	0.0	0.0	6.7160065745322335	0.0
38	6.624881331813144E-5	0.0	0.0	6.935058275768635	0.0
39	6.624881331813144E-5	0.0	0.0	7.218040081857033	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATCG	20	7.0351124E-4	45.0	24
GTCGTAA	20	7.0351124E-4	45.0	8
TATCTCG	60	3.6379788E-12	41.249996	34
CGTTTTT	8605	0.0	40.39802	1
TAACGGG	805	0.0	40.248447	3
CGTAAGG	535	0.0	39.532715	2
CGTTAGG	600	0.0	39.375	2
GGCGATA	885	0.0	38.898304	8
GGGCGAT	4310	0.0	38.578884	7
ACGCGAA	35	6.2516683E-6	38.571426	21
ATCGATT	35	6.2516683E-6	38.571426	23
CGTGAGT	300	0.0	38.25	32
ATAGGGA	4040	0.0	38.09406	4
GTAGGGC	1440	0.0	37.8125	4
CACGACC	275	0.0	37.636364	27
AGGGCGA	2360	0.0	37.372883	6
TAGGGAT	4665	0.0	37.33119	5
TAAGGGA	4070	0.0	36.81818	4
TATGGGA	3215	0.0	36.671852	4
AGGGATT	4695	0.0	36.565495	6
>>END_MODULE