##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547591_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1754849
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.405328891545654	31.0	31.0	33.0	30.0	34.0
2	31.819998187878273	31.0	31.0	34.0	30.0	34.0
3	31.95296917284621	31.0	31.0	34.0	30.0	34.0
4	35.71766117768537	37.0	35.0	37.0	33.0	37.0
5	35.63728788060967	37.0	35.0	37.0	33.0	37.0
6	35.67828855930054	37.0	35.0	37.0	33.0	37.0
7	35.959464888431995	37.0	35.0	37.0	35.0	37.0
8	35.98808045592527	37.0	35.0	37.0	35.0	37.0
9	37.85215195153543	39.0	38.0	39.0	35.0	39.0
10	37.27094980821712	39.0	37.0	39.0	34.0	39.0
11	36.94940476360074	39.0	37.0	39.0	33.0	39.0
12	36.06270397054106	37.0	35.0	39.0	32.0	39.0
13	35.71633627736632	37.0	35.0	39.0	31.0	39.0
14	36.69535441510922	38.0	35.0	41.0	31.0	41.0
15	36.99377097402682	38.0	35.0	41.0	32.0	41.0
16	37.15418078706487	38.0	35.0	41.0	33.0	41.0
17	37.0681295085788	38.0	35.0	41.0	32.0	41.0
18	37.011033997796964	38.0	35.0	41.0	32.0	41.0
19	36.93941643981904	38.0	35.0	41.0	32.0	41.0
20	36.75248810581423	38.0	35.0	40.0	32.0	41.0
21	36.55112548145168	38.0	35.0	40.0	31.0	41.0
22	36.46172234762079	38.0	35.0	40.0	31.0	41.0
23	36.446896000738526	38.0	35.0	40.0	31.0	41.0
24	36.35209981029707	37.0	35.0	40.0	31.0	41.0
25	36.25813218117342	37.0	35.0	40.0	31.0	41.0
26	36.156440240727264	37.0	35.0	40.0	31.0	41.0
27	36.059559540450486	37.0	35.0	40.0	31.0	41.0
28	36.10171074548295	37.0	35.0	40.0	31.0	41.0
29	36.18134779687597	37.0	35.0	40.0	31.0	41.0
30	36.22605021856582	37.0	35.0	40.0	31.0	41.0
31	36.09968550000598	37.0	35.0	40.0	31.0	41.0
32	35.936650389862606	37.0	35.0	40.0	30.0	41.0
33	35.799267059445	37.0	35.0	40.0	30.0	41.0
34	35.7094576228496	37.0	35.0	40.0	30.0	41.0
35	35.54579054950027	37.0	35.0	40.0	30.0	41.0
36	35.385887902605866	37.0	34.0	40.0	29.0	41.0
37	35.34454018550884	37.0	34.0	40.0	29.0	41.0
38	35.28866301317093	37.0	34.0	40.0	29.0	41.0
39	35.2622510540793	37.0	34.0	40.0	29.0	41.0
40	35.13523442757753	36.0	34.0	40.0	27.0	41.0
41	35.11533869865726	36.0	34.0	40.0	28.0	41.0
42	35.07968491875939	36.0	34.0	40.0	27.0	41.0
43	35.02315355908115	36.0	34.0	40.0	27.0	41.0
44	34.89030794102513	36.0	34.0	40.0	27.0	41.0
45	34.80169404888968	36.0	34.0	40.0	27.0	41.0
46	34.77389906481982	36.0	34.0	40.0	27.0	41.0
47	34.73780821027906	35.0	34.0	40.0	27.0	41.0
48	34.629550462746366	35.0	34.0	40.0	26.0	41.0
49	34.59576635938477	36.0	34.0	40.0	26.0	41.0
50	34.50545659484092	35.0	34.0	40.0	26.0	41.0
51	34.0860165176605	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	1.0
12	1.0
13	2.0
14	9.0
15	32.0
16	69.0
17	238.0
18	525.0
19	1120.0
20	2052.0
21	3370.0
22	5170.0
23	8045.0
24	12117.0
25	17650.0
26	24234.0
27	28800.0
28	31867.0
29	35767.0
30	42856.0
31	52391.0
32	66587.0
33	89640.0
34	166645.0
35	282861.0
36	115913.0
37	139991.0
38	215263.0
39	411446.0
40	184.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.634345747126964	24.110678468631775	29.988449148616205	14.266526635625059
2	29.957905210077907	26.86989022987163	30.10834550437103	13.063859055679433
3	27.934426266875384	26.264311060381836	32.454587260784265	13.346675411958522
4	25.153047356211278	28.394408863668612	31.370334427634518	15.082209352485599
5	23.40184255169533	33.434101737528415	29.07651883438404	14.087536876392214
6	22.04052884322241	42.069431614913874	25.861712318267838	10.028327223595875
7	88.72398707809049	3.654160557404084	6.45371767029528	1.1681346942101571
8	89.50114796201838	2.898824913140675	5.8461440272069	1.7538830976340414
9	84.84314034996744	5.592674925306964	7.332425752871045	2.2317589718545583
10	47.40476246104366	30.430082588302472	12.858941139665008	9.306213810988865
11	42.73997363875752	20.026566388333126	23.699304042684012	13.534155930225337
12	42.33674806208397	19.766771955877687	24.15848885003781	13.737991132000532
13	21.302573611746652	38.96244064304108	24.074379049137562	15.660606696074705
14	14.906581705890364	40.91109833381675	26.746232866759478	17.436087093533402
15	13.832244255773574	26.49384647909877	44.418636589245004	15.255272675882656
16	15.760102436164022	21.063635674636394	45.24879348593526	17.927468403264328
17	15.77343691679455	23.50128130682469	27.615310491102086	33.109971285278675
18	20.16817401383253	25.05543211980062	34.86339850323304	19.91299536313381
19	26.67158256921251	26.936847557824063	26.118315592965548	20.27325427999788
20	30.12954390947597	23.695315095486848	27.53188450972135	18.64325648531583
21	21.355398669629125	29.622206810956385	29.115553531956312	19.906840987458178
22	22.506551845771344	24.331894083194623	27.475355429441507	25.686198641592522
23	19.027278130483023	30.677454299486733	26.28112162356989	24.01414594646035
24	19.191109890366636	24.53664104432917	39.682274657249714	16.58997440805448
25	16.738249273869148	27.289755414853357	35.43387493738778	20.538120373889722
26	17.054173891884712	36.01409579969559	26.95474083525135	19.976989473168345
27	16.591000137333754	36.857701146936286	28.557499819072753	17.993798896657207
28	14.51207482809062	29.978818690383047	38.37310218713974	17.136004294386584
29	14.884528526386031	24.513847060345363	37.86473935934089	22.736885053927715
30	16.49429666028245	33.12695280334662	32.35845363333256	18.02029690303838
31	27.28388596397753	28.928186983609415	25.336367972401042	18.45155908001201
32	27.984060167000123	28.145270618725597	26.060076963886917	17.810592250387355
33	25.696057039665522	30.378682154418986	24.438854853038638	19.486405952876858
34	18.01699177536073	28.64548459725025	28.32847726499545	25.009046362393573
35	18.9849383052331	26.777232684977452	32.09563899799926	22.142190011790188
36	27.549606832268758	27.492336947509443	27.62625160341431	17.331804616807485
37	18.402779954286665	33.307652111378246	30.695404561873985	17.594163372461104
38	18.19393007603503	33.42430032441538	26.763271369787372	21.61849822976222
39	18.977017395798725	33.7291698601988	28.16430359535208	19.1295091486504
40	23.658559796312957	27.894137900184003	27.32656769898721	21.12073460451583
41	16.646503488334325	25.63229086947082	30.621893963526208	27.09931167866865
42	20.681836442907624	25.44794452400178	26.676369305849107	27.19384972724149
43	21.21880572060616	26.83666799821523	26.824188291984097	25.120337989194514
44	17.81577788174367	30.3437503739638	29.00346411571594	22.83700762857659
45	16.258891790689685	38.08185205678665	24.050616320834443	21.60863983168922
46	21.137887077463645	34.777807093373845	25.546186594971985	18.53811923419052
47	20.091643212606897	28.761733915567667	28.09426907956183	23.05235379226361
48	21.277728169204302	25.16182304004504	33.05224552083968	20.50820326991097
49	20.006678637307253	25.544989910812838	32.36779916676591	22.08053228511399
50	18.113410327612232	31.815728874678108	30.22670326620695	19.84415753150271
51	17.12369554303533	33.19049103370148	26.165100245092315	23.520713178170887
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1000.0
1	1918.5
2	2837.0
3	10503.0
4	18169.0
5	12801.0
6	7433.0
7	7395.0
8	7357.0
9	7839.5
10	8322.0
11	8416.5
12	8511.0
13	8374.0
14	8237.0
15	7642.5
16	7048.0
17	6884.5
18	6721.0
19	6713.0
20	6705.0
21	6908.5
22	7112.0
23	7902.0
24	8692.0
25	11194.0
26	14751.5
27	15807.0
28	22788.0
29	29769.0
30	32485.0
31	35201.0
32	38582.5
33	41964.0
34	50421.5
35	58879.0
36	61516.0
37	64153.0
38	72167.5
39	80182.0
40	107775.5
41	135369.0
42	162214.5
43	189060.0
44	191717.5
45	194375.0
46	186503.0
47	178631.0
48	159479.0
49	140327.0
50	128345.5
51	116364.0
52	102299.5
53	88235.0
54	77643.5
55	67052.0
56	60368.5
57	53685.0
58	47200.5
59	40716.0
60	37252.5
61	33789.0
62	29639.0
63	25489.0
64	21952.5
65	18416.0
66	15647.0
67	12878.0
68	10225.5
69	7573.0
70	6760.0
71	5947.0
72	4893.5
73	3840.0
74	3175.0
75	1969.0
76	1428.0
77	1173.5
78	919.0
79	562.5
80	206.0
81	152.5
82	99.0
83	87.0
84	75.0
85	65.5
86	56.0
87	32.5
88	9.0
89	4.5
90	0.0
91	1.5
92	3.0
93	3.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1754849.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.251250594433824
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.27594101599156	12.883331835604336
2	8.23794387189167	2.6775378048998393
3	2.7582947655354073	1.3447721834409299
4	1.30980259291925	0.851437206670797
5	0.7424213137753718	0.6032637408406175
6	0.48768378904874965	0.47552828800045394
7	0.36354220522567443	0.4135610835142868
8	0.262933149894818	0.34183940068196145
9	0.20248789959351848	0.29616134387713416
>10	2.9961480437833523	12.390615861554048
>50	1.5545460422619448	18.3434403101108
>100	1.7972553268345288	47.1477733200991
>500	0.008516116060025397	0.8660225826635906
>1k	0.002129029015006349	0.6720353694474287
>5k	0.0	0.0
>10k+	3.548381691677249E-4	0.6926796685946898
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12012	0.6845033390337288	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4499	0.25637533485787095	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2017	0.11493866423834757	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.036242434534253375	0.0
2	0.0	0.0	0.0	0.14417194869758024	0.0
3	0.0	0.0	0.0	0.21283882544879928	0.0
4	0.0	0.0	0.0	0.45553776991638595	0.0
5	0.0	0.0	0.0	0.7451923213906154	0.0
6	0.0	0.0	0.0	1.1369069361523414	0.0
7	0.0	0.0	0.0	1.3005107561961171	0.0
8	0.0	0.0	0.0	1.77177637506133	0.0
9	0.0	0.0	0.0	1.9043803768871281	0.0
10	0.0	0.0	0.0	2.1598439523856467	0.0
11	0.0	0.0	0.0	2.516056937092593	0.0
12	0.0	0.0	0.0	2.7952832408942307	0.0
13	0.0	0.0	0.0	2.910221905132578	0.0
14	0.0	0.0	0.0	2.95136504622335	0.0
15	0.0	0.0	0.0	3.0195190583349336	0.0
16	0.0	0.0	0.0	3.184490517417738	0.0
17	0.0	0.0	0.0	3.367127314087993	0.0
18	0.0	0.0	0.0	3.6047545971191823	0.0
19	0.0	0.0	0.0	3.72561969719332	5.6984959959517884E-5
20	0.0	0.0	0.0	3.859363398218308	5.6984959959517884E-5
21	0.0	0.0	0.0	4.0470148713650005	5.6984959959517884E-5
22	0.0	0.0	0.0	4.244410772664771	5.6984959959517884E-5
23	0.0	0.0	0.0	4.460725680671101	5.6984959959517884E-5
24	0.0	0.0	0.0	4.62854638775188	5.6984959959517884E-5
25	0.0	0.0	0.0	4.768387479492538	5.6984959959517884E-5
26	0.0	0.0	0.0	4.8971165040410884	5.6984959959517884E-5
27	0.0	0.0	0.0	5.019064318354457	5.6984959959517884E-5
28	0.0	0.0	0.0	5.1626094324924825	5.6984959959517884E-5
29	0.0	0.0	0.0	5.310827313347188	5.6984959959517884E-5
30	0.0	0.0	0.0	5.501214064571937	5.6984959959517884E-5
31	0.0	0.0	0.0	5.672909748929965	5.6984959959517884E-5
32	0.0	0.0	0.0	5.830416178258073	5.6984959959517884E-5
33	0.0	0.0	0.0	5.984788434788406	5.6984959959517884E-5
34	0.0	0.0	0.0	6.157566833385665	5.6984959959517884E-5
35	0.0	0.0	0.0	6.3780416434690395	5.6984959959517884E-5
36	0.0	0.0	0.0	6.564553417416541	5.6984959959517884E-5
37	0.0	0.0	0.0	6.747361168966675	5.6984959959517884E-5
38	0.0	0.0	0.0	6.9249263042005325	5.6984959959517884E-5
39	0.0	0.0	0.0	7.1049417927126495	5.6984959959517884E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	180	0.0	45.0	25
AACGTAG	40	6.8193913E-9	45.0	1
TCGTTAG	105	0.0	45.0	1
CGTATCC	20	7.034304E-4	45.0	32
GGTCGTA	45	3.8562575E-10	45.0	8
CGACGAA	35	1.212411E-7	45.0	20
CGTGCCG	20	7.034304E-4	45.0	39
CTATACG	55	1.8189894E-12	45.0	1
CATCTCG	35	1.212411E-7	45.0	31
CCGCTCG	25	3.8916485E-5	45.0	19
TCTAGCG	90	0.0	45.0	1
CCGTCTA	25	3.8916485E-5	45.0	30
CCGTCCA	20	7.034304E-4	45.0	19
ACCTACG	20	7.034304E-4	45.0	1
TCGTGCA	35	1.212411E-7	45.0	20
CTACGTT	20	7.034304E-4	45.0	30
CGCTAAT	35	1.212411E-7	45.0	36
TGCGAAT	25	3.8916485E-5	45.0	15
TCGCTAA	35	1.212411E-7	45.0	35
CGAATTC	20	7.034304E-4	45.0	25
>>END_MODULE