FastQCFastQC Report
Thu 26 May 2016
SRR1547590_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547590_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2270963
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139320.6134842355423669No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC60040.26438123386422413No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAACT207.0347416E-445.00000428
CGCATCA207.0347416E-445.00000415
CTCGATG551.8189894E-1245.0000041
ACGGTAC207.0347416E-445.00000425
ACGTTAT253.8920127E-545.028
CGACCGG253.8920127E-545.032
TCGATAG700.045.01
GTAGCGT253.8920127E-545.036
GTCGTAC453.8562575E-1045.026
TCGGTAA351.2126293E-745.029
GCGTAAT253.8920127E-545.039
ACTAACG302.1664418E-644.99999624
TTCGCGA302.1664418E-644.9999961
CGTTTTT84550.042.6848031
CGGTCTA2200.041.9318231
GAATACG650.041.5384641
ACTACGG650.041.5384642
GTCCCAT650.041.53846444
GGCAACC603.6379788E-1241.2499968
TCGATTG603.6379788E-1241.2499961
Produced by FastQC (version 0.11.5)