##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547590_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2270963
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.375379519613485	31.0	31.0	33.0	30.0	34.0
2	31.77427637526459	31.0	31.0	34.0	30.0	34.0
3	31.880442349787295	31.0	31.0	34.0	30.0	34.0
4	35.65654834534953	37.0	35.0	37.0	33.0	37.0
5	35.573226424208585	37.0	35.0	37.0	33.0	37.0
6	35.61674892985927	37.0	35.0	37.0	33.0	37.0
7	35.94902294753371	37.0	35.0	37.0	35.0	37.0
8	35.987178566977974	37.0	35.0	37.0	35.0	37.0
9	37.83571286718454	39.0	38.0	39.0	35.0	39.0
10	37.2500670420434	39.0	37.0	39.0	34.0	39.0
11	36.88579910813166	39.0	37.0	39.0	33.0	39.0
12	36.00659059614798	37.0	35.0	39.0	32.0	39.0
13	35.67376967392247	37.0	35.0	39.0	31.0	39.0
14	36.500936386898424	38.0	35.0	41.0	31.0	41.0
15	36.83208665222639	38.0	35.0	41.0	32.0	41.0
16	36.996377748118306	38.0	35.0	41.0	32.0	41.0
17	36.95520754851576	38.0	35.0	41.0	32.0	41.0
18	36.884731719539246	38.0	35.0	40.0	32.0	41.0
19	36.82248543899659	38.0	35.0	40.0	32.0	41.0
20	36.61939362288157	38.0	35.0	40.0	32.0	41.0
21	36.40861519980731	38.0	35.0	40.0	31.0	41.0
22	36.396894621356665	38.0	35.0	40.0	31.0	41.0
23	36.358995280856625	37.0	35.0	40.0	31.0	41.0
24	36.27398596982866	37.0	35.0	40.0	31.0	41.0
25	36.14581127037297	37.0	35.0	40.0	31.0	41.0
26	36.02437071850136	37.0	35.0	40.0	31.0	41.0
27	35.94753106941857	37.0	35.0	40.0	31.0	41.0
28	35.99931130538014	37.0	35.0	40.0	31.0	41.0
29	36.03908209865154	37.0	35.0	40.0	31.0	41.0
30	36.05855181260109	37.0	35.0	40.0	31.0	41.0
31	35.88720952300852	37.0	35.0	40.0	30.0	41.0
32	35.73221668516836	37.0	34.0	40.0	30.0	41.0
33	35.578046405863944	37.0	34.0	40.0	30.0	41.0
34	35.45736368227928	37.0	34.0	40.0	29.0	41.0
35	35.299463267345175	37.0	34.0	40.0	29.0	41.0
36	35.143880811796585	37.0	34.0	40.0	27.0	41.0
37	35.08184061122969	37.0	34.0	40.0	27.0	41.0
38	35.01073993719845	37.0	34.0	40.0	27.0	41.0
39	34.97371379454443	36.0	34.0	40.0	27.0	41.0
40	34.84804640146053	36.0	34.0	40.0	26.0	41.0
41	34.769798539210015	36.0	34.0	40.0	25.0	41.0
42	34.75327823482813	36.0	34.0	40.0	26.0	41.0
43	34.720138989494764	36.0	34.0	40.0	26.0	41.0
44	34.63239691707879	36.0	34.0	40.0	26.0	41.0
45	34.52482581178117	35.0	34.0	40.0	25.0	41.0
46	34.507344681529375	35.0	34.0	40.0	25.0	41.0
47	34.44677478232803	35.0	34.0	40.0	25.0	41.0
48	34.36116528538774	35.0	34.0	40.0	24.0	41.0
49	34.32502687186009	36.0	34.0	40.0	24.0	41.0
50	34.20497647914123	35.0	34.0	40.0	24.0	41.0
51	33.7651718676174	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	3.0
13	5.0
14	15.0
15	42.0
16	143.0
17	360.0
18	874.0
19	1675.0
20	3001.0
21	4857.0
22	7963.0
23	12194.0
24	17827.0
25	25925.0
26	34643.0
27	39924.0
28	43355.0
29	48747.0
30	57686.0
31	71511.0
32	90427.0
33	121057.0
34	216646.0
35	349581.0
36	156688.0
37	191441.0
38	284391.0
39	489804.0
40	175.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.646034743850954	23.94975171325997	29.29558958027938	13.108623962609695
2	30.721680626236537	26.489291106900463	29.910438875490264	12.878589391372735
3	29.4681155087071	24.85007461592285	32.08951444827591	13.592295427094145
4	25.80993173380632	28.948556185195446	30.837446492963554	14.404065588034678
5	23.21077005657952	32.577633365228756	30.173939425697384	14.037657152494337
6	21.993753310820125	40.36393371446386	27.59296386598989	10.04934910872612
7	87.73872581807805	3.7881286485072634	7.143357245362431	1.3297882880522491
8	88.35071289140333	2.9803215640237206	6.70407223719629	1.9648933073766504
9	84.02849363904211	5.076128497029674	8.494546146282437	2.400831717645774
10	50.03701073069002	26.70320916721232	13.638839558372373	9.620940543725283
11	43.603308376226295	21.369612803026733	22.289354780328875	12.737724040418097
12	39.61411964880097	21.904275851257815	24.711983418488103	13.76962108145311
13	22.549464698456116	37.843945498011195	26.135212242559653	13.471377560973034
14	16.281286837346094	39.758948076212604	29.31703422732999	14.64273085911131
15	14.808827796842133	25.365186486966103	45.91866974494961	13.907315971242157
16	18.32064194793134	22.115860099878333	43.084893941468884	16.478604010721444
17	18.868779456116194	22.04465682620104	28.363738202691984	30.722825514990777
18	23.262730392348974	24.172432575960066	34.752613759008845	17.812223272682118
19	28.60614637931133	26.503470113779926	26.531079546430302	18.35930396047844
20	29.668603143248042	26.438035318056702	27.22637929371813	16.66698224497713
21	22.084463727502385	28.58029831397517	30.892753426630026	18.44248453189242
22	22.566638029769752	23.905233154393095	29.20144449733439	24.326684318502767
23	18.840201271442997	29.758212705358915	28.329083300784735	23.072502722413354
24	20.524464731481753	25.396847064439182	37.40122582358233	16.67746238049673
25	18.47934114294244	26.39312045154412	35.15314868626217	19.97438971925126
26	17.10336099707481	35.281244124188724	27.423432261996343	20.19196261674012
27	17.232293084475618	35.54787110137858	30.94555041187373	16.274285402272078
28	15.24745229226544	29.99956406158973	37.55935257421631	17.193631071928518
29	16.083793527239326	26.77930023518657	37.135963906061	20.00094233151311
30	19.330389794990054	31.749922830094548	32.029099549398204	16.89058782551719
31	27.88284089172743	27.526208044781004	27.007529404926455	17.58342165856511
32	29.01632479261001	27.937883620296766	27.389217701917644	15.656573885175584
33	27.506877038507454	29.080438562847565	25.306092613574066	18.106591785070915
34	18.767060493720063	29.97195462893935	29.15247848599911	22.10850639134147
35	19.43919826082591	28.122915256655435	31.312046915779785	21.12583956673887
36	29.64011302694055	25.596454015323015	27.55179190502003	17.211641052716402
37	19.29908149097982	32.963769114688354	30.046372397965094	17.69077699636674
38	20.29865744179892	32.9001397204622	25.478838712916062	21.32236412482282
39	19.513880234948786	31.72667278154686	28.569686075907004	20.18976090759735
40	24.86112719581957	26.74763965771349	27.276534227990506	21.114698918476435
41	17.837851167104	25.181167636813107	29.39845343142975	27.582527764653147
42	21.62135622641144	25.38029021168553	27.50264975695333	25.495703804949706
43	21.53535746729471	25.85004687438765	28.154840039225647	24.45975561909199
44	19.29058289368871	30.946827403176535	28.883825936397905	20.878763766736842
45	17.35466407863096	36.93600468171432	24.431045331870223	21.278285907784493
46	21.995866951597186	32.88199763712575	26.052648149705654	19.069487261571414
47	19.46760030876769	29.182157525243696	28.64855129740115	22.70169086858747
48	22.20758330276627	26.990664312892815	31.1273235187011	19.67442886563982
49	21.452705306075	25.428683778643684	31.85723413371332	21.261376781567996
50	19.11669190559247	32.4229412808575	28.49443165740701	19.96593515614301
51	17.9590772725051	34.057225943355306	25.572763624946777	22.41093315919282
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1332.0
1	2344.0
2	3356.0
3	12286.5
4	21217.0
5	15189.5
6	9162.0
7	9612.5
8	10063.0
9	11333.0
10	12603.0
11	13674.0
12	14745.0
13	14095.0
14	13445.0
15	13072.0
16	12699.0
17	11645.0
18	10591.0
19	10642.0
20	10693.0
21	11130.0
22	11567.0
23	12267.5
24	12968.0
25	14915.5
26	18996.0
27	21129.0
28	26365.5
29	31602.0
30	35092.0
31	38582.0
32	47152.5
33	55723.0
34	62634.0
35	69545.0
36	76156.5
37	82768.0
38	92713.0
39	102658.0
40	134848.0
41	167038.0
42	193615.0
43	220192.0
44	226528.5
45	232865.0
46	223275.0
47	213685.0
48	202442.5
49	191200.0
50	182498.5
51	173797.0
52	155014.0
53	136231.0
54	119441.0
55	102651.0
56	90995.0
57	79339.0
58	69680.0
59	60021.0
60	49476.0
61	38931.0
62	35235.5
63	31540.0
64	26790.5
65	22041.0
66	18769.5
67	15498.0
68	11851.0
69	8204.0
70	7032.0
71	5860.0
72	4757.0
73	3654.0
74	3023.5
75	1932.0
76	1471.0
77	987.0
78	503.0
79	356.0
80	209.0
81	196.0
82	183.0
83	134.0
84	85.0
85	56.5
86	28.0
87	26.5
88	25.0
89	14.0
90	3.0
91	4.0
92	5.0
93	2.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2270963.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.85786703017108
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.57364035786296	14.031576215872008
2	8.85204907405798	3.1615743061815285
3	3.143012114851823	1.6838247726372195
4	1.5229050939451843	1.087833466689732
5	0.8709795456385757	0.7776918456006255
6	0.6215865340668403	0.6660125803866324
7	0.45835723228191805	0.5729697754485411
8	0.34994178388987496	0.4999371076005001
9	0.2955279451225826	0.47497488729288984
>10	2.675903113447362	11.022825257063818
>50	1.0663226681984086	13.936307869138354
>100	1.5600445291828458	49.94467452943724
>500	0.007983595859448099	0.8866962519598336
>1k	0.0012474368530387653	0.36489007655265987
>5k	2.494873706077531E-4	0.26749694989281686
>10k+	2.494873706077531E-4	0.6207141082456236
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13932	0.6134842355423669	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6004	0.26438123386422413	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.403418285546704E-5	0.0	0.0	0.041788439529838226	0.0
2	4.403418285546704E-5	0.0	0.0	0.14192217134317028	0.0
3	4.403418285546704E-5	0.0	0.0	0.2267320075227998	0.0
4	4.403418285546704E-5	0.0	0.0	0.45830777515970095	0.0
5	4.403418285546704E-5	0.0	0.0	0.7881238047471492	0.0
6	4.403418285546704E-5	0.0	0.0	1.21107213107391	0.0
7	4.403418285546704E-5	0.0	0.0	1.422920584791562	0.0
8	4.403418285546704E-5	0.0	0.0	1.9891561421300128	0.0
9	4.403418285546704E-5	0.0	0.0	2.165336907734736	0.0
10	4.403418285546704E-5	0.0	0.0	2.495769415882161	0.0
11	4.403418285546704E-5	0.0	0.0	2.9704138728812404	0.0
12	4.403418285546704E-5	0.0	0.0	3.3249330790506053	0.0
13	4.403418285546704E-5	0.0	0.0	3.4724916257992753	0.0
14	4.403418285546704E-5	0.0	0.0	3.527402251820043	0.0
15	4.403418285546704E-5	0.0	0.0	3.6122120879996724	0.0
16	4.403418285546704E-5	0.0	0.0	3.805654253283739	0.0
17	4.403418285546704E-5	0.0	0.0	4.023095048224035	0.0
18	4.403418285546704E-5	0.0	0.0	4.304825750133314	0.0
19	4.403418285546704E-5	0.0	0.0	4.465022107361502	0.0
20	4.403418285546704E-5	0.0	0.0	4.643492650474711	0.0
21	4.403418285546704E-5	0.0	0.0	4.878503084374338	0.0
22	4.403418285546704E-5	0.0	0.0	5.1269879782277386	0.0
23	4.403418285546704E-5	0.0	0.0	5.395024049268967	0.0
24	4.403418285546704E-5	0.0	0.0	5.586132402861693	0.0
25	4.403418285546704E-5	0.0	0.0	5.759891288409366	0.0
26	4.403418285546704E-5	0.0	0.0	5.91867855178618	0.0
27	4.403418285546704E-5	0.0	0.0	6.099570974956439	0.0
28	4.403418285546704E-5	0.0	0.0	6.26082415257316	0.0
29	4.403418285546704E-5	0.0	0.0	6.478529152610588	0.0
30	4.403418285546704E-5	0.0	0.0	6.7673493579595965	0.0
31	4.403418285546704E-5	0.0	0.0	7.016847038018673	0.0
32	4.403418285546704E-5	0.0	0.0	7.2178630827538806	0.0
33	4.403418285546704E-5	0.0	0.0	7.42389902433461	0.0
34	4.403418285546704E-5	0.0	0.0	7.681631096587659	0.0
35	4.403418285546704E-5	0.0	0.0	7.971860395788043	0.0
36	4.403418285546704E-5	0.0	0.0	8.194409155939573	0.0
37	4.403418285546704E-5	0.0	0.0	8.439415349347392	0.0
38	4.403418285546704E-5	0.0	0.0	8.737879040741747	0.0
39	4.403418285546704E-5	0.0	0.0	9.326219757873643	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAACT	20	7.0347416E-4	45.000004	28
CGCATCA	20	7.0347416E-4	45.000004	15
CTCGATG	55	1.8189894E-12	45.000004	1
ACGGTAC	20	7.0347416E-4	45.000004	25
ACGTTAT	25	3.8920127E-5	45.0	28
CGACCGG	25	3.8920127E-5	45.0	32
TCGATAG	70	0.0	45.0	1
GTAGCGT	25	3.8920127E-5	45.0	36
GTCGTAC	45	3.8562575E-10	45.0	26
TCGGTAA	35	1.2126293E-7	45.0	29
GCGTAAT	25	3.8920127E-5	45.0	39
ACTAACG	30	2.1664418E-6	44.999996	24
TTCGCGA	30	2.1664418E-6	44.999996	1
CGTTTTT	8455	0.0	42.684803	1
CGGTCTA	220	0.0	41.93182	31
GAATACG	65	0.0	41.538464	1
ACTACGG	65	0.0	41.538464	2
GTCCCAT	65	0.0	41.538464	44
GGCAACC	60	3.6379788E-12	41.249996	8
TCGATTG	60	3.6379788E-12	41.249996	1
>>END_MODULE