FastQCFastQC Report
Thu 26 May 2016
SRR1547589_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547589_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences579964
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33750.58193267168307No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC22810.3933002738100985No Hit
GAGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCT6880.11862805277568954No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGATC1100.045.00000416
CGTATGG551.8189894E-1245.0000042
GCACGAG302.1636351E-645.0000041
AGGGTAC1200.045.0000046
TCGATTG302.1636351E-645.0000041
TTATCGG302.1636351E-645.00000427
TACGAAT302.1636351E-645.00000412
GCGATAC302.1636351E-645.0000049
ATGCGAG600.045.0000041
TACTACG302.1636351E-645.0000041
CCAGTAG1100.045.00000426
CAATACG302.1636351E-645.00000444
GGTACGA207.0303935E-445.019
ACCGGGT207.0303935E-445.04
CCCTCAT207.0303935E-445.032
CGACGGT351.2105374E-745.028
GCGAATG351.2105374E-745.01
CCCTATA207.0303935E-445.013
ACGTTAG351.2105374E-745.01
TGGAACG351.2105374E-745.01
Produced by FastQC (version 0.11.5)