##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1547589_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 579964 Sequences flagged as poor quality 0 Sequence length 51 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.37111441399811 31.0 31.0 33.0 30.0 34.0 2 31.786671241663274 31.0 31.0 34.0 30.0 34.0 3 31.866981398845446 31.0 31.0 34.0 30.0 34.0 4 35.623016256181415 37.0 35.0 37.0 33.0 37.0 5 35.5280758805719 37.0 35.0 37.0 33.0 37.0 6 35.58482078197957 37.0 35.0 37.0 33.0 37.0 7 35.945124180121525 37.0 35.0 37.0 35.0 37.0 8 35.995749736190525 37.0 35.0 37.0 35.0 37.0 9 37.858994696222524 39.0 38.0 39.0 35.0 39.0 10 37.265932023366965 39.0 37.0 39.0 34.0 39.0 11 36.83079294576905 39.0 35.0 39.0 33.0 39.0 12 35.46470643005428 35.0 35.0 39.0 31.0 39.0 13 34.89821092343663 35.0 34.0 39.0 30.0 39.0 14 35.74808091536716 36.0 35.0 40.0 30.0 41.0 15 36.212025229152154 36.0 35.0 40.0 31.0 41.0 16 36.47987806139692 36.0 35.0 40.0 32.0 41.0 17 36.410149595492136 36.0 35.0 40.0 32.0 41.0 18 36.33095674903959 36.0 35.0 40.0 32.0 41.0 19 36.242587470946475 36.0 35.0 40.0 31.0 41.0 20 36.00734873199026 36.0 35.0 40.0 31.0 41.0 21 35.77058058776062 35.0 34.0 40.0 31.0 41.0 22 35.68323723541461 35.0 34.0 40.0 31.0 41.0 23 35.73907001124208 35.0 34.0 40.0 31.0 41.0 24 35.620804049906546 35.0 34.0 40.0 31.0 41.0 25 35.514749191329116 35.0 34.0 40.0 31.0 41.0 26 35.39269161534164 35.0 34.0 40.0 30.0 41.0 27 35.327991047720204 35.0 34.0 40.0 30.0 41.0 28 35.40914091219455 36.0 34.0 40.0 30.0 41.0 29 35.53775406749384 36.0 35.0 40.0 31.0 41.0 30 35.57379423550427 36.0 35.0 40.0 31.0 41.0 31 35.3318947382941 35.0 34.0 40.0 30.0 41.0 32 35.08910035795325 35.0 34.0 40.0 29.0 41.0 33 34.89467449703775 35.0 34.0 40.0 29.0 41.0 34 34.82242863350139 35.0 34.0 40.0 29.0 41.0 35 34.63331344704154 35.0 34.0 40.0 28.0 41.0 36 34.400438647916076 35.0 34.0 40.0 25.0 41.0 37 34.317712823554565 35.0 34.0 40.0 25.0 41.0 38 34.324508072914874 35.0 33.0 40.0 27.0 41.0 39 34.33946762212827 35.0 34.0 40.0 26.0 41.0 40 34.190841155657935 35.0 34.0 40.0 24.0 41.0 41 34.188960004414064 35.0 34.0 40.0 24.0 41.0 42 34.142884730776395 35.0 34.0 40.0 24.0 41.0 43 34.0596209419895 35.0 33.0 40.0 24.0 41.0 44 33.93589946962225 35.0 33.0 39.0 24.0 41.0 45 33.84060562379734 35.0 33.0 39.0 23.0 41.0 46 33.83489147602265 35.0 33.0 39.0 23.0 41.0 47 33.800299673772855 35.0 33.0 39.0 23.0 41.0 48 33.73491458090502 35.0 33.0 39.0 23.0 41.0 49 33.751619066010996 35.0 33.0 39.0 24.0 41.0 50 33.60916022373802 35.0 33.0 39.0 24.0 41.0 51 33.16615858915381 35.0 33.0 38.0 23.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 3.0 13 1.0 14 6.0 15 13.0 16 35.0 17 95.0 18 230.0 19 475.0 20 888.0 21 1400.0 22 2325.0 23 3532.0 24 5248.0 25 7609.0 26 10161.0 27 11728.0 28 12422.0 29 13490.0 30 16255.0 31 19579.0 32 25103.0 33 33637.0 34 67122.0 35 121091.0 36 32605.0 37 37506.0 38 56065.0 39 101308.0 40 32.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 29.726155416543094 23.83544495865261 30.970370574725326 15.468029050078972 2 29.752708788821376 27.212206274872237 29.578387624059427 13.456697312246968 3 27.95001069031871 25.655902780172564 32.20337814071218 14.190708388796548 4 24.92378837307143 27.901904256126244 32.06974915684422 15.104558213958107 5 23.018670124352546 32.63357725651937 31.24280127732066 13.104951341807421 6 22.141546716692762 39.97127407908077 27.36945741459815 10.517721789628322 7 88.01029029388032 3.474181156071756 7.182342352283936 1.3331861977639992 8 89.43037843728231 2.381009855784152 6.261423122814519 1.9271885841190144 9 85.80687766826907 4.40785979819437 8.037223000048279 1.7480395334882854 10 57.84600423474561 22.953493665124043 11.2082818933589 7.992220206771456 11 54.15146457366319 15.79339407273555 20.162285934989068 9.89285541861219 12 49.61739004489934 18.450283121021304 20.965439234159362 10.966887599919994 13 19.85209426792008 47.803829203191924 21.315633384141083 11.028443144746914 14 11.714347787104028 50.14725051899773 25.823671814112597 12.314729879785641 15 10.088729645288328 23.42559193329241 55.55672421046823 10.928954210951025 16 12.47370526446469 16.797077059955445 54.56959397479844 16.159623700781427 17 12.954942030884675 18.246477367560747 27.816209281955434 40.982371319599146 18 20.172803829203193 22.820209530246704 37.2617955597244 19.745191080825705 19 29.592354008179818 25.504686497782618 25.59314026387845 19.309819230159114 20 33.23499389617287 22.08878482112683 26.85408059810609 17.822140684594213 21 19.67070369885027 30.066004096806008 29.38061672793484 20.88267547640888 22 20.943024049768606 24.003903690573896 27.056334531108828 27.99673772854867 23 16.750177597230174 30.73725265706147 24.977067542123304 27.53550220358505 24 18.919105323778716 22.8022773827341 42.80645005552069 15.472167237966495 25 14.09242642646785 24.462207999117187 39.8950624521522 21.55030312226276 26 13.968108365346815 39.05483788648951 27.82793414763675 19.14911960052693 27 14.696429433551048 39.17105199633081 30.292742308143268 15.83977626197488 28 11.752281176072996 30.62534915960301 43.33182749274093 14.290542171583065 29 11.29552868798753 23.496630825361574 43.26647860901711 21.941361877633785 30 15.48130573621811 33.39621079929099 34.29092150547275 16.831561959018146 31 30.149629977033054 28.51659758191888 24.639632804794783 16.694139636253286 32 31.570407818416314 26.838045120041933 27.66016511369671 13.93138194784504 33 28.755405507928078 29.167672476222663 23.57629094219641 18.500631073652848 34 18.17233483457594 29.17629370098834 27.872764516418258 24.77860694801746 35 17.614886441227387 26.67096578408315 33.191542923353865 22.5226048513356 36 32.38338931381948 22.797449496865323 28.971625825051213 15.84753536426399 37 18.147678131746108 33.08688125469857 32.90652523260064 15.85891538095468 38 18.09370926471298 35.009241952948805 23.673538357553227 23.223510424784987 39 17.820244015145768 34.09694394824506 29.950479684945964 18.13233235166321 40 24.754122669682946 26.145243497872283 25.80298087467498 23.29765295776979 41 15.034381444365513 23.256788352380493 31.4859198157127 30.222910387541297 42 23.275755046864976 23.68146988433765 25.44606216937603 27.596712899421345 43 23.1917843176473 24.24374616355498 26.822354490968404 25.742115027829314 44 17.632991013235305 32.781517473498354 28.76471643067501 20.820775082591332 45 15.13473250063797 42.531088136505026 21.996537716134103 20.3376416467229 46 23.32765481995434 34.988551013511184 24.620838534805607 17.062955631728865 47 20.06934913201509 29.211123449041665 27.20375747460187 23.515769944341372 48 24.163741197729514 23.2428219682601 33.05394817609369 19.5394886579167 49 20.56955259291956 22.333800028967314 34.32109579215262 22.775551585960507 50 18.252339800401405 35.02872592091923 28.17105889331062 18.547875385368748 51 16.114276058513976 36.91384292818175 23.946141484643874 23.0257395286604 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 365.0 1 480.5 2 596.0 3 2866.5 4 5137.0 5 3665.5 6 2194.0 7 2461.5 8 2729.0 9 3071.5 10 3414.0 11 3588.5 12 3763.0 13 3717.0 14 3671.0 15 3385.0 16 3099.0 17 2821.0 18 2543.0 19 2461.5 20 2380.0 21 2381.0 22 2382.0 23 2588.0 24 2794.0 25 3183.0 26 3995.0 27 4418.0 28 5487.5 29 6557.0 30 7634.0 31 8711.0 32 9939.0 33 11167.0 34 12286.0 35 13405.0 36 14395.0 37 15385.0 38 19044.0 39 22703.0 40 33747.5 41 44792.0 42 59735.0 43 74678.0 44 76294.0 45 77910.0 46 71764.0 47 65618.0 48 59567.0 49 53516.0 50 47551.5 51 41587.0 52 36476.5 53 31366.0 54 25349.5 55 19333.0 56 17446.0 57 15559.0 58 13371.5 59 11184.0 60 9507.5 61 7831.0 62 6873.0 63 5915.0 64 4742.0 65 3569.0 66 3090.0 67 2611.0 68 1920.0 69 1229.0 70 1167.0 71 1105.0 72 840.0 73 575.0 74 407.5 75 186.0 76 132.0 77 119.0 78 106.0 79 93.5 80 81.0 81 52.0 82 23.0 83 18.0 84 13.0 85 8.5 86 4.0 87 2.0 88 0.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 579964.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.9366857727243 #Duplication Level Percentage of deduplicated Percentage of total 1 80.63424141380263 16.075795335889755 2 6.429296234375078 2.5635771752899106 3 2.017589704958345 1.2067215589811415 4 0.997481808920843 0.7954592555385387 5 0.6240589601150844 0.6220833695733762 6 0.44236469744712 0.5291571581969692 7 0.39195215176256615 0.5469958821343398 8 0.28071010416953707 0.44771433120454124 9 0.26854300486768884 0.48184717540592553 >10 4.832904683135445 25.76747330636442 >50 2.55072383327732 36.25618188834983 >100 0.5249189762519596 13.331104259280705 >500 0.0034762846109401297 0.39590820541007277 >1k 0.0017381423054700649 0.979981098380469 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3375 0.58193267168307 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC 2281 0.3933002738100985 No Hit GAGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCT 688 0.11862805277568954 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.044485519790883575 0.0 2 0.0 0.0 0.0 0.19915029208709506 0.0 3 0.0 0.0 0.0 0.30639832817209345 0.0 4 0.0 0.0 0.0 0.6948707161134139 0.0 5 0.0 0.0 0.0 1.1012752515673387 0.0 6 0.0 0.0 0.0 1.6859667151754247 0.0 7 0.0 0.0 0.0 1.8985661178969728 0.0 8 0.0 0.0 0.0 2.498948210578588 0.0 9 0.0 0.0 0.0 2.6725796773592845 0.0 10 0.0 0.0 0.0 2.9910477202033228 0.0 11 0.0 0.0 0.0 3.4262471463746027 0.0 12 0.0 0.0 0.0 3.7466118586670896 0.0 13 0.0 0.0 0.0 3.8779993240959785 0.0 14 0.0 0.0 0.0 3.93076121966191 0.0 15 0.0 0.0 0.0 4.006800422095164 0.0 16 0.0 0.0 0.0 4.1914670565759256 0.0 17 0.0 0.0 0.0 4.384410066831735 0.0 18 0.0 0.0 0.0 4.671152002538088 0.0 19 0.0 0.0 0.0 4.7875385368747025 0.0 20 0.0 0.0 0.0 4.929271472022402 0.0 21 0.0 0.0 0.0 5.13255995199702 0.0 22 0.0 0.0 0.0 5.316881737487154 0.0 23 0.0 0.0 0.0 5.524135980853984 0.0 24 0.0 0.0 0.0 5.677938630673628 0.0 25 0.0 0.0 0.0 5.803980936747799 0.0 26 0.0 0.0 0.0 5.926402328420385 0.0 27 0.0 0.0 0.0 6.0348573359725775 0.0 28 0.0 0.0 0.0 6.154002662234207 0.0 29 0.0 0.0 0.0 6.282803760233394 0.0 30 0.0 0.0 0.0 6.448848549220297 0.0 31 1.7242449531350222E-4 0.0 0.0 6.614548489216572 0.0 32 1.7242449531350222E-4 0.0 0.0 6.753005358953314 0.0 33 1.7242449531350222E-4 0.0 0.0 6.889565559241608 0.0 34 1.7242449531350222E-4 0.0 0.0 7.04129911511749 0.0 35 1.7242449531350222E-4 0.0 0.0 7.2632094405859675 0.0 36 1.7242449531350222E-4 0.0 0.0 7.4156326944431035 0.0 37 1.7242449531350222E-4 0.0 0.0 7.576849597561228 0.0 38 1.7242449531350222E-4 0.0 0.0 7.7246173900449 0.0 39 1.7242449531350222E-4 0.0 0.0 7.873419729500451 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTGATC 110 0.0 45.000004 16 CGTATGG 55 1.8189894E-12 45.000004 2 GCACGAG 30 2.1636351E-6 45.000004 1 AGGGTAC 120 0.0 45.000004 6 TCGATTG 30 2.1636351E-6 45.000004 1 TTATCGG 30 2.1636351E-6 45.000004 27 TACGAAT 30 2.1636351E-6 45.000004 12 GCGATAC 30 2.1636351E-6 45.000004 9 ATGCGAG 60 0.0 45.000004 1 TACTACG 30 2.1636351E-6 45.000004 1 CCAGTAG 110 0.0 45.000004 26 CAATACG 30 2.1636351E-6 45.000004 44 GGTACGA 20 7.0303935E-4 45.0 19 ACCGGGT 20 7.0303935E-4 45.0 4 CCCTCAT 20 7.0303935E-4 45.0 32 CGACGGT 35 1.2105374E-7 45.0 28 GCGAATG 35 1.2105374E-7 45.0 1 CCCTATA 20 7.0303935E-4 45.0 13 ACGTTAG 35 1.2105374E-7 45.0 1 TGGAACG 35 1.2105374E-7 45.0 1 >>END_MODULE