FastQCFastQC Report
Thu 26 May 2016
SRR1547588_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547588_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2856201
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT354521.2412291711962848No Hit
GAATCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTC31840.11147674831008042No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTCG207.035048E-445.01
TAGTACG1000.045.01
CGTTTTT206450.043.4088171
TACGGGA10200.039.4852944
GCGATAC1500.039.09
TACCCGC356.2515483E-638.5714341
CGTTAGG4200.038.571432
ACGTAGG3800.038.4868432
CGGGATC7800.038.3653836
ACGGGAT12750.038.2941175
TTACGGG7000.038.253
GGCACCG9300.038.2258078
TAGGGAC29350.037.947195
AGGGATC32500.037.9384656
AGTACGG4450.037.921352
ACGGGTA2500.037.8000035
AGGGCGA21100.037.5355456
TCGATAC301.14034636E-437.50000423
GTTAGCG1750.037.2857131
ATAGGGA36600.037.25414
Produced by FastQC (version 0.11.5)