##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547588_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2856201
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.234219160346207	31.0	31.0	33.0	30.0	34.0
2	31.629414036337078	31.0	31.0	34.0	30.0	34.0
3	31.625819751481075	31.0	31.0	34.0	30.0	34.0
4	35.49800626776617	37.0	35.0	37.0	33.0	37.0
5	35.437019663532084	37.0	35.0	37.0	33.0	37.0
6	35.49172239628793	37.0	35.0	37.0	33.0	37.0
7	35.87980362726573	37.0	35.0	37.0	35.0	37.0
8	35.91006970447808	37.0	35.0	37.0	35.0	37.0
9	37.74455614293252	39.0	37.0	39.0	35.0	39.0
10	37.068424806237374	39.0	37.0	39.0	33.0	39.0
11	36.7229949852969	39.0	35.0	39.0	32.0	39.0
12	36.32794645754973	38.0	35.0	39.0	32.0	39.0
13	36.19956158547665	38.0	35.0	39.0	32.0	39.0
14	37.24630374402922	39.0	35.0	41.0	32.0	41.0
15	37.42976807304528	39.0	35.0	41.0	32.0	41.0
16	37.4880619396184	39.0	35.0	41.0	32.0	41.0
17	37.3657099062706	39.0	35.0	41.0	32.0	41.0
18	37.20925243006357	39.0	35.0	41.0	32.0	41.0
19	37.11414112662239	38.0	35.0	41.0	32.0	41.0
20	36.90126080062293	38.0	35.0	41.0	32.0	41.0
21	36.754196570899595	38.0	35.0	41.0	31.0	41.0
22	36.70663444204382	38.0	35.0	41.0	31.0	41.0
23	36.61140690028468	38.0	35.0	40.0	31.0	41.0
24	36.50860916301059	38.0	35.0	40.0	31.0	41.0
25	36.4157046370336	38.0	35.0	40.0	31.0	41.0
26	36.30631667729267	38.0	35.0	40.0	31.0	41.0
27	36.21373110645924	38.0	35.0	40.0	30.0	41.0
28	36.193937681556726	38.0	35.0	40.0	30.0	41.0
29	36.20360786933413	38.0	35.0	40.0	30.0	41.0
30	36.15353471271805	38.0	35.0	40.0	30.0	41.0
31	36.01004586161828	38.0	35.0	40.0	30.0	41.0
32	35.78734584855898	38.0	35.0	40.0	29.0	41.0
33	35.548225422510534	38.0	34.0	40.0	27.0	41.0
34	35.32236456747967	38.0	34.0	40.0	26.0	41.0
35	35.067493849347436	38.0	34.0	40.0	25.0	41.0
36	34.95773441715061	38.0	34.0	40.0	24.0	41.0
37	34.92556476242393	38.0	34.0	40.0	24.0	41.0
38	34.826173998258525	38.0	34.0	40.0	23.0	41.0
39	34.769159803529234	38.0	34.0	40.0	23.0	41.0
40	34.69915177538275	38.0	34.0	40.0	23.0	41.0
41	34.60930970894555	38.0	34.0	40.0	23.0	41.0
42	34.569219043057544	38.0	34.0	40.0	23.0	41.0
43	34.526467149895964	38.0	34.0	40.0	23.0	41.0
44	34.39262888011033	37.0	33.0	40.0	23.0	41.0
45	34.301802989355444	37.0	33.0	40.0	23.0	41.0
46	34.27679739626168	37.0	33.0	40.0	23.0	41.0
47	34.208087245960634	37.0	33.0	40.0	23.0	41.0
48	34.063939127533395	37.0	33.0	40.0	22.0	41.0
49	34.00639625852662	37.0	33.0	40.0	22.0	41.0
50	33.92348157570143	37.0	33.0	40.0	22.0	41.0
51	33.512837156768725	36.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	8.0
11	2.0
12	5.0
13	5.0
14	24.0
15	68.0
16	197.0
17	622.0
18	1401.0
19	2733.0
20	4811.0
21	7594.0
22	12139.0
23	18389.0
24	27739.0
25	41615.0
26	55763.0
27	62496.0
28	63867.0
29	67026.0
30	74208.0
31	88168.0
32	106764.0
33	135459.0
34	210671.0
35	301961.0
36	214172.0
37	264969.0
38	397888.0
39	695246.0
40	191.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.763626229386514	23.385223939071516	28.264677450921695	17.586472380620272
2	33.68393891046183	25.144483879110744	28.219197458442174	12.95237975198524
3	26.458747125990083	25.06651317606849	35.255291906977135	13.219447790964292
4	23.41988536521064	27.197000491211927	35.31929300493908	14.063821138638351
5	21.958398586093907	31.411689863563524	33.100786674327196	13.529124876015377
6	20.224136886724708	39.83077521504964	29.71475046749161	10.230337430734043
7	85.83559070247507	2.479202269027985	10.286635989553956	1.3985710389429875
8	86.3973508867198	2.1151872714840447	9.809883828203967	1.6775780135921805
9	82.14596941881891	4.247950336828535	11.130484164104697	2.4755960802478536
10	41.56325132579955	30.668814974856463	17.34023620886625	10.427697490477735
11	28.883996609482317	24.049707986237664	30.943515529894427	16.12277987438559
12	29.268738439626624	21.851893476684587	31.184219878082807	17.695148205605978
13	22.115110246092627	28.32920372200696	32.22871919728338	17.326966834617032
14	16.923773922073412	31.87398225825143	32.23011965894557	18.972124160729585
15	16.45447921907457	26.701097016631532	40.42908744867746	16.415336315616443
16	19.6629718986864	24.607161750871175	38.98044990531129	16.74941644513114
17	19.802702961031105	25.12417718500904	31.637024145009402	23.436095708950454
18	21.099215356342217	25.575160851774786	34.483322427238136	18.842301364644857
19	23.787436528451604	27.97800294867203	29.96112668541185	18.27343383746452
20	25.255925615879278	26.803925914177608	30.233131351750103	17.707017118193015
21	21.78176535895058	27.95125413092426	32.46189606403751	17.80508444608765
22	21.982346480517304	23.984131368905757	32.325561121223615	21.707961029353324
23	19.184224079467796	28.245141010734187	31.889352324993936	20.68128258480408
24	19.47583520907667	25.943832384345498	36.4937551663906	18.086577240187225
25	18.980701988410477	28.088464362277026	33.25056604909808	19.68026760021441
26	18.59368440806512	31.59150914098833	30.56437554639887	19.250430904547684
27	18.35284001371052	30.954474142401047	32.14448142830284	18.548204415585598
28	17.325881476828837	28.259635788937825	35.96756670836541	18.446916025867928
29	17.359842672136867	26.037348211838037	35.9525117454969	20.650297370528197
30	19.1134657539858	28.177673770158336	33.31813832429861	19.39072215155726
31	22.872199820670886	26.968095032527472	31.08534728473241	19.07435786206923
32	23.435920651242682	26.560560688831075	31.32297061726398	18.680548042662267
33	22.2733974254613	27.869362135227878	30.456014825287152	19.401225614023662
34	18.618297521778054	28.068402748966196	31.57690932816003	21.736390401095722
35	19.151523299655732	28.36179946719436	31.9771262596715	20.509550973478408
36	22.28085488381245	27.76772363009466	30.025617944955556	19.925803541137334
37	19.113045615487145	30.156736168077806	31.702285658467318	19.027932557967734
38	19.10310233768562	30.679633541196853	29.700045620038644	20.51721850107888
39	19.38039374679863	29.681174399140676	30.273254578371763	20.665177275688933
40	21.43228015115183	28.216501569742462	29.786804220011128	20.56441405909458
41	17.9575246980167	27.689157730845977	30.708973213019675	23.644344358117653
42	20.215944186000915	27.92450531317649	29.120429549601024	22.73912095122157
43	20.65736970192224	27.727425345765234	29.499849625429025	22.115355326883506
44	19.67907720780155	29.678723591231847	29.486860343512237	21.15533885745436
45	18.626700291751174	31.664018043548054	28.161813541834064	21.547468122866704
46	20.62015243324962	30.113636960424007	28.693463800341785	20.572746805984593
47	19.950521689474936	28.63709521843876	30.191117501884495	21.22126559020181
48	20.250010415933613	27.215521596694348	31.47863193101606	21.05583605635598
49	20.273012998735034	26.604115046525088	31.214889988484707	21.90798196625518
50	18.690736401254675	29.33375487229365	31.223572850790266	20.751935875661413
51	17.915335790443322	29.964347747234875	29.523377381353765	22.59693908096804
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1560.0
1	2351.0
2	3142.0
3	28562.5
4	53983.0
5	37903.0
6	21823.0
7	21610.0
8	21397.0
9	22719.5
10	24042.0
11	24857.0
12	25672.0
13	25237.0
14	24802.0
15	23975.5
16	23149.0
17	21572.5
18	19996.0
19	19882.0
20	19768.0
21	18607.0
22	17446.0
23	18633.5
24	19821.0
25	21860.0
26	28096.5
27	32294.0
28	38646.0
29	44998.0
30	51069.0
31	57140.0
32	65928.5
33	74717.0
34	82909.5
35	91102.0
36	103038.5
37	114975.0
38	126851.0
39	138727.0
40	158465.0
41	178203.0
42	199557.0
43	220911.0
44	229484.5
45	238058.0
46	248068.5
47	258079.0
48	250211.5
49	242344.0
50	225804.0
51	209264.0
52	189318.5
53	169373.0
54	149799.5
55	130226.0
56	116288.0
57	102350.0
58	90707.5
59	79065.0
60	70923.5
61	62782.0
62	52995.0
63	43208.0
64	34631.5
65	26055.0
66	20960.5
67	15866.0
68	13180.5
69	10495.0
70	8649.0
71	6803.0
72	5193.5
73	3584.0
74	2966.0
75	1927.0
76	1506.0
77	1096.5
78	687.0
79	493.5
80	300.0
81	216.0
82	132.0
83	78.0
84	24.0
85	26.5
86	29.0
87	34.0
88	39.0
89	24.0
90	9.0
91	7.5
92	6.0
93	3.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2856201.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.57981449276789
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.03549939008488	17.620271000786182
2	7.906709340907965	3.5706406033187386
3	2.9098380574874487	1.9711081062618798
4	1.569734611152664	1.4177726529081716
5	1.041017486855899	1.175299086846682
6	0.6916542567089617	0.9370454885772971
7	0.5402660740553552	0.8539375410234143
8	0.426750034194293	0.7708749285511592
9	0.3807581615954373	0.7737703789889081
>10	4.228714538059173	22.982865212819707
>50	1.6037443047955944	25.94010537576827
>100	0.6608859104257033	19.510313269362424
>500	0.0018976430042846946	0.28954824552174513
>1k	0.0023720537553558684	0.9205627472926515
>5k	0.0	0.0
>10k+	1.5813691702372455E-4	1.265885361972735
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35452	1.2412291711962848	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTC	3184	0.11147674831008042	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.5011541554673495E-5	0.0	0.0	0.047370615723473244	0.0
2	3.5011541554673495E-5	0.0	0.0	0.16112311423460743	0.0
3	3.5011541554673495E-5	0.0	0.0	0.30474045769187813	0.0
4	3.5011541554673495E-5	0.0	0.0	0.5143195454381537	0.0
5	3.5011541554673495E-5	0.0	0.0	0.9873604833833474	0.0
6	3.5011541554673495E-5	0.0	0.0	1.6582166311124462	0.0
7	3.5011541554673495E-5	0.0	0.0	2.031089548669719	0.0
8	3.5011541554673495E-5	0.0	0.0	2.6916873147232985	0.0
9	3.5011541554673495E-5	0.0	0.0	3.0072463387555706	0.0
10	3.5011541554673495E-5	0.0	0.0	3.491806073872252	0.0
11	3.5011541554673495E-5	0.0	0.0	3.9056424950484927	0.0
12	3.5011541554673495E-5	0.0	0.0	4.269167331010667	0.0
13	3.5011541554673495E-5	0.0	0.0	4.449301712309463	0.0
14	3.5011541554673495E-5	0.0	0.0	4.547299017120994	0.0
15	3.5011541554673495E-5	0.0	0.0	4.654119230404302	0.0
16	3.5011541554673495E-5	0.0	0.0	4.836004188780832	0.0
17	3.5011541554673495E-5	0.0	0.0	5.044532930280467	0.0
18	3.5011541554673495E-5	0.0	0.0	5.293044852235539	0.0
19	3.5011541554673495E-5	0.0	0.0	5.465021544352096	0.0
20	3.5011541554673495E-5	0.0	0.0	5.624674173841407	0.0
21	3.5011541554673495E-5	0.0	0.0	5.839680050528656	0.0
22	3.5011541554673495E-5	0.0	0.0	6.074467448194297	0.0
23	3.5011541554673495E-5	0.0	0.0	6.357675807830051	0.0
24	3.5011541554673495E-5	0.0	0.0	6.56690478016078	0.0
25	3.5011541554673495E-5	0.0	0.0	6.765455232317334	0.0
26	3.5011541554673495E-5	0.0	0.0	6.944259175037051	0.0
27	3.5011541554673495E-5	0.0	0.0	7.128174802823751	0.0
28	7.002308310934699E-5	0.0	0.0	7.321018373706893	0.0
29	7.002308310934699E-5	0.0	0.0	7.546457689777435	0.0
30	7.002308310934699E-5	0.0	0.0	7.794339403984523	0.0
31	1.050346246640205E-4	0.0	0.0	8.035253821422232	0.0
32	1.050346246640205E-4	0.0	0.0	8.261743483739414	0.0
33	1.050346246640205E-4	0.0	0.0	8.490788988590088	0.0
34	1.050346246640205E-4	0.0	0.0	8.715002900706217	0.0
35	1.050346246640205E-4	0.0	0.0	8.96666586140121	0.0
36	1.050346246640205E-4	0.0	0.0	9.214547575608298	0.0
37	1.050346246640205E-4	0.0	0.0	9.46417986689312	0.0
38	1.050346246640205E-4	0.0	0.0	9.734398944612092	0.0
39	1.050346246640205E-4	0.0	0.0	10.080207940547602	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTCG	20	7.035048E-4	45.0	1
TAGTACG	100	0.0	45.0	1
CGTTTTT	20645	0.0	43.408817	1
TACGGGA	1020	0.0	39.485294	4
GCGATAC	150	0.0	39.0	9
TACCCGC	35	6.2515483E-6	38.57143	41
CGTTAGG	420	0.0	38.57143	2
ACGTAGG	380	0.0	38.486843	2
CGGGATC	780	0.0	38.365383	6
ACGGGAT	1275	0.0	38.294117	5
TTACGGG	700	0.0	38.25	3
GGCACCG	930	0.0	38.225807	8
TAGGGAC	2935	0.0	37.94719	5
AGGGATC	3250	0.0	37.938465	6
AGTACGG	445	0.0	37.92135	2
ACGGGTA	250	0.0	37.800003	5
AGGGCGA	2110	0.0	37.535545	6
TCGATAC	30	1.14034636E-4	37.500004	23
GTTAGCG	175	0.0	37.285713	1
ATAGGGA	3660	0.0	37.2541	4
>>END_MODULE