FastQCFastQC Report
Thu 26 May 2016
SRR1547587_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547587_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1745827
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80580.4615577603050016No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC25440.14571890571058874No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAA253.8916405E-545.018
TAGCACG207.034295E-445.01
CGGTCGA207.034295E-445.017
TAGTACG650.041.5384641
CGTTTTT48450.040.3095971
CGTTAGG1900.040.2631572
TAGGTCG451.9292202E-840.01
TGTACGA451.9292202E-840.012
GTATGCG403.4595905E-739.3751
TCGTCCC6400.039.37538
TAATGCG800.039.3751
GTACGCG403.4595905E-739.3751
GACCGAT8450.038.875749
GCGAATA356.2501513E-638.5714341
GCGATCG356.2501513E-638.571439
AGGGTAC6100.038.3606576
CGTAAGG3550.038.028172
GGGCGAT23350.037.8693777
TACGCGG1250.037.8000032
CCAATCG301.14016446E-437.50000424
Produced by FastQC (version 0.11.5)