##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547587_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1745827
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.284684564965485	31.0	31.0	33.0	30.0	34.0
2	31.6997938512808	31.0	31.0	34.0	30.0	34.0
3	31.851100939554723	31.0	31.0	34.0	30.0	34.0
4	35.60970187767746	37.0	35.0	37.0	33.0	37.0
5	35.50682685054132	37.0	35.0	37.0	33.0	37.0
6	35.55209193121655	37.0	35.0	37.0	33.0	37.0
7	35.88088854164817	37.0	35.0	37.0	35.0	37.0
8	35.905081660439436	37.0	35.0	37.0	35.0	37.0
9	37.74947288591596	39.0	37.0	39.0	35.0	39.0
10	37.084604030067126	39.0	37.0	39.0	33.0	39.0
11	36.800577605913986	39.0	35.0	39.0	32.0	39.0
12	36.10789213364211	38.0	35.0	39.0	32.0	39.0
13	35.84109250229261	38.0	35.0	39.0	31.0	39.0
14	36.72008051198658	39.0	35.0	41.0	31.0	41.0
15	37.0009010056552	39.0	35.0	41.0	32.0	41.0
16	37.10740182160088	39.0	35.0	41.0	32.0	41.0
17	37.03422389503656	38.0	35.0	41.0	32.0	41.0
18	36.957672209216604	38.0	35.0	41.0	32.0	41.0
19	36.924068650559306	38.0	35.0	41.0	32.0	41.0
20	36.773068007311146	38.0	35.0	41.0	31.0	41.0
21	36.59518268419494	38.0	35.0	41.0	31.0	41.0
22	36.56202075005141	38.0	35.0	40.0	31.0	41.0
23	36.511865150441594	38.0	35.0	40.0	31.0	41.0
24	36.42231045802362	38.0	35.0	40.0	31.0	41.0
25	36.30444769155248	38.0	35.0	40.0	31.0	41.0
26	36.185221674312515	38.0	35.0	40.0	30.0	41.0
27	36.09904360512239	38.0	35.0	40.0	30.0	41.0
28	36.14417808866514	38.0	35.0	40.0	30.0	41.0
29	36.165571961024774	38.0	35.0	40.0	30.0	41.0
30	36.18528410890655	38.0	35.0	40.0	30.0	41.0
31	36.09967826136267	38.0	35.0	40.0	30.0	41.0
32	36.002665785326954	38.0	35.0	40.0	30.0	41.0
33	35.89191254345362	38.0	35.0	40.0	30.0	41.0
34	35.791628265572704	38.0	35.0	40.0	29.0	41.0
35	35.63566722246821	38.0	34.0	40.0	29.0	41.0
36	35.49879569968846	38.0	34.0	40.0	28.0	41.0
37	35.443373827990975	38.0	34.0	40.0	28.0	41.0
38	35.36758624995489	38.0	34.0	40.0	28.0	41.0
39	35.30418248772645	37.0	34.0	40.0	27.0	41.0
40	35.19872186648505	37.0	34.0	40.0	27.0	41.0
41	35.11811021366951	37.0	34.0	40.0	27.0	41.0
42	35.08540536948965	37.0	34.0	40.0	27.0	41.0
43	35.03037013403963	37.0	34.0	40.0	27.0	41.0
44	34.910956240223115	37.0	34.0	40.0	26.0	41.0
45	34.82323506280977	37.0	34.0	40.0	26.0	41.0
46	34.818229412192615	37.0	34.0	40.0	26.0	41.0
47	34.76415074345854	36.0	34.0	40.0	26.0	41.0
48	34.63771725377142	36.0	34.0	40.0	26.0	41.0
49	34.58292201919205	36.0	34.0	40.0	26.0	41.0
50	34.48545588995931	36.0	34.0	40.0	25.0	41.0
51	34.05440115200418	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	2.0
12	4.0
13	6.0
14	12.0
15	48.0
16	117.0
17	338.0
18	748.0
19	1382.0
20	2460.0
21	3988.0
22	6192.0
23	9024.0
24	13349.0
25	18419.0
26	24683.0
27	28969.0
28	32964.0
29	38170.0
30	45396.0
31	55248.0
32	68447.0
33	89274.0
34	151569.0
35	223787.0
36	125087.0
37	158925.0
38	237380.0
39	409699.0
40	138.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.14384930465619	24.26248419803337	29.822027039334365	13.771639457976075
2	30.587853206531918	26.088610154385282	30.05721643668015	13.266320202402643
3	29.138912389371914	25.645439095626315	31.157554557238488	14.058093957763285
4	25.80553514179813	28.369534896642108	30.60893204194917	15.21599791961059
5	23.16970696409209	32.46209389590148	30.2533985326152	14.114800607391226
6	22.146810651914535	41.379815984057984	26.252314805533423	10.221058558494054
7	89.65619159286688	3.2384079293080017	5.7566986877852155	1.3487017900399068
8	90.50272449675712	2.4254980590860376	5.363131627589675	1.7086458165671627
9	85.97850760699657	4.647654091728447	6.998345196860857	2.375493104414126
10	44.95749006058447	28.36363511390304	14.25313046481696	12.425744360695532
11	39.256409712989885	22.83399214240586	23.039052552171547	14.870545592432698
12	37.61747297985424	20.933975703205416	24.627354256750525	16.821197060189814
13	23.7064726344592	32.951260348247565	26.174930276596708	17.16733674069653
14	17.991874338064427	35.23063854551453	28.169916034062943	18.607571082358103
15	17.473552648687413	25.755186510461805	40.57618538377514	16.195075457075646
16	20.760648105453747	22.79366741378155	38.53085099497258	17.91483348579212
17	21.17122716053767	23.081496620226403	27.259802947256517	28.487473271979415
18	23.24262369639145	24.983689678301456	32.4744662558203	19.299220369486783
19	27.61848682601426	26.488535232872444	26.217202506319353	19.67577543479394
20	29.234912737630935	25.672990508223325	26.86423110651857	18.22786564762717
21	23.60835294676964	27.799432589827056	29.548517693906668	19.043696769496634
22	23.569460204247044	23.56499240760969	28.875541505544366	23.990005882598904
23	20.813459752885024	28.11275114888245	27.73877365855838	23.335015439674148
24	21.127981180265856	24.993484463237195	35.47430530058248	18.404229055914474
25	20.456150580784925	26.785128194259798	31.915533440598637	20.84318778435664
26	19.212556570610946	32.03759593590888	27.497627199029456	21.252220294450712
27	18.383264779385357	32.64745017690756	29.86028970797221	19.108995335734868
28	17.734804193084425	29.037928729478924	34.32928921365061	18.897977863786046
29	17.88436082154761	26.63058825416264	34.071531715341784	21.413519208947964
30	19.79067799959561	29.852442424134807	31.563379418464717	18.793500157804868
31	25.848609283737733	27.177148709465488	27.483880132452988	19.490361874343794
32	27.09764484109823	26.724297424658914	27.33168864956264	18.846369084680212
33	25.832971995507	28.06475097475294	26.033965564743816	20.068311464996246
34	19.977351707815263	27.75452550567725	29.598007133581966	22.670115652925517
35	20.82818057001066	27.73911733522279	30.04850996118172	21.38419213358483
36	27.109215288800094	26.42163284220029	27.31965996630823	19.14949190269139
37	20.952820640303994	30.818803925016624	29.33308970476456	18.89528572991482
38	21.249413601691348	29.877301702860592	27.40569369129931	21.46759100414875
39	20.457639846330707	29.932289969166476	28.764705781271573	20.845364403231248
40	24.646141914405035	26.223961480719453	27.44200885883882	21.68788774603669
41	18.966025843339576	24.966792242301214	29.685014609122213	26.382167305237004
42	21.827363192343803	25.83302927495107	27.035267526507496	25.304340006197634
43	21.87914380978184	25.775978948658718	28.469430247097794	23.87544699446165
44	20.480723462290364	28.607931942855735	29.002243635824172	21.909100959029733
45	18.328333792523544	33.05327503813379	25.793735576319992	22.824655593022676
46	21.524412212664828	30.936054947025106	26.803973131358376	20.73555970895169
47	20.43621733424904	28.37331533995064	28.576313689729854	22.61415363607047
48	21.66079456899223	26.28954644417803	31.274175505362216	20.77548348146752
49	21.673109649466987	25.459968255732097	30.77103286866339	22.095889226137526
50	19.865484953549235	31.02437984977893	28.668705433012548	20.441429763659286
51	18.917681992545653	31.66539410835094	26.83307108894524	22.583852810158167
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1006.0
1	1673.5
2	2341.0
3	7920.5
4	13500.0
5	9662.0
6	5824.0
7	5964.5
8	6105.0
9	6626.0
10	7147.0
11	7147.0
12	7147.0
13	7350.0
14	7553.0
15	7174.5
16	6796.0
17	6569.0
18	6342.0
19	6497.0
20	6652.0
21	6882.0
22	7112.0
23	7915.5
24	8719.0
25	9913.5
26	12659.5
27	14211.0
28	18579.5
29	22948.0
30	27529.0
31	32110.0
32	36842.0
33	41574.0
34	49340.0
35	57106.0
36	58126.5
37	59147.0
38	67440.0
39	75733.0
40	98186.0
41	120639.0
42	134804.5
43	148970.0
44	156533.0
45	164096.0
46	158913.5
47	153731.0
48	148856.5
49	143982.0
50	136712.0
51	129442.0
52	117710.0
53	105978.0
54	96437.0
55	86896.0
56	80030.0
57	73164.0
58	65833.0
59	58502.0
60	52374.5
61	46247.0
62	41585.0
63	36923.0
64	31619.0
65	26315.0
66	22120.5
67	17926.0
68	15063.5
69	12201.0
70	10465.5
71	8730.0
72	6797.0
73	4864.0
74	3970.0
75	2640.5
76	2205.0
77	1439.5
78	674.0
79	633.5
80	593.0
81	440.0
82	287.0
83	198.0
84	109.0
85	69.5
86	30.0
87	33.5
88	37.0
89	25.0
90	13.0
91	11.5
92	10.0
93	7.0
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1745827.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.700536466479722
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.7835577172438	14.545942575838481
2	7.337923527172434	2.7444621301625527
3	2.4809851709059205	1.391872609839648
4	1.2893389923769942	0.9644532337840082
5	0.8774987060131689	0.8204848275544019
6	0.6355931878546098	0.7131560152392736
7	0.48620884573699746	0.6364656375020816
8	0.39562912841909004	0.5918781554562227
9	0.3452998468455083	0.5811563140023862
>10	5.333855221357424	25.624857449648204
>50	2.1330924748362134	28.527966430930686
>100	0.8976042373696511	21.853935184815253
>500	0.0024821409951180755	0.32097610106254615
>1k	6.205352487795189E-4	0.21485439302867107
>5k	3.1026762438975944E-4	0.4675389411355742
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8058	0.4615577603050016	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2544	0.14571890571058874	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.023026336515588313	0.0
2	0.0	0.0	0.0	0.09617218659122583	0.0
3	0.0	0.0	0.0	0.15998148728367703	0.0
4	0.0	0.0	0.0	0.3417864427575012	0.0
5	0.0	0.0	0.0	0.5910665833441687	0.0
6	0.0	0.0	0.0	1.0213497671876997	0.0
7	0.0	0.0	0.0	1.2035556787700041	0.0
8	0.0	0.0	0.0	1.6635096146410842	0.0
9	0.0	0.0	0.0	1.840445817369075	0.0
10	0.0	0.0	0.0	2.1346330421055466	0.0
11	0.0	0.0	0.0	2.4783669859613813	0.0
12	0.0	0.0	0.0	2.732057643741333	0.0
13	0.0	0.0	0.0	2.863113011770353	0.0
14	0.0	0.0	0.0	2.921595324164422	0.0
15	0.0	0.0	0.0	2.9925645553654516	0.0
16	0.0	0.0	0.0	3.136164121645501	0.0
17	0.0	0.0	0.0	3.28978759063756	0.0
18	0.0	0.0	0.0	3.4825901993725608	0.0
19	0.0	0.0	0.0	3.6047672535709436	0.0
20	0.0	0.0	0.0	3.7337032821694245	0.0
21	0.0	0.0	0.0	3.9031931571684937	0.0
22	0.0	0.0	0.0	4.0813322282219255	0.0
23	0.0	0.0	0.0	4.271385423641632	0.0
24	0.0	0.0	0.0	4.4138966804843776	0.0
25	0.0	0.0	0.0	4.545238445733741	0.0
26	0.0	0.0	0.0	4.670623148799967	0.0
27	0.0	0.0	0.0	4.815139186185115	0.0
28	0.0	0.0	0.0	4.9526671313938895	0.0
29	0.0	0.0	0.0	5.11419516366742	0.0
30	0.0	0.0	0.0	5.329222196701048	0.0
31	5.7279444068627646E-5	0.0	0.0	5.506444796649381	0.0
32	1.1455888813725529E-4	0.0	0.0	5.668373785031392	0.0
33	1.1455888813725529E-4	0.0	0.0	5.829386302308304	0.0
34	1.1455888813725529E-4	0.0	0.0	6.007926329470217	0.0
35	1.1455888813725529E-4	0.0	0.0	6.2315452791141395	0.0
36	1.1455888813725529E-4	0.0	0.0	6.4102571446082575	0.0
37	1.7183833220588295E-4	0.0	0.0	6.610334242739974	0.0
38	1.7183833220588295E-4	0.0	0.0	6.844950845645073	0.0
39	1.7183833220588295E-4	0.0	0.0	7.287778227739633	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGAAA	25	3.8916405E-5	45.0	18
TAGCACG	20	7.034295E-4	45.0	1
CGGTCGA	20	7.034295E-4	45.0	17
TAGTACG	65	0.0	41.538464	1
CGTTTTT	4845	0.0	40.309597	1
CGTTAGG	190	0.0	40.263157	2
TAGGTCG	45	1.9292202E-8	40.0	1
TGTACGA	45	1.9292202E-8	40.0	12
GTATGCG	40	3.4595905E-7	39.375	1
TCGTCCC	640	0.0	39.375	38
TAATGCG	80	0.0	39.375	1
GTACGCG	40	3.4595905E-7	39.375	1
GACCGAT	845	0.0	38.87574	9
GCGAATA	35	6.2501513E-6	38.57143	41
GCGATCG	35	6.2501513E-6	38.57143	9
AGGGTAC	610	0.0	38.360657	6
CGTAAGG	355	0.0	38.02817	2
GGGCGAT	2335	0.0	37.869377	7
TACGCGG	125	0.0	37.800003	2
CCAATCG	30	1.14016446E-4	37.500004	24
>>END_MODULE