FastQCFastQC Report
Thu 26 May 2016
SRR1547586_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547586_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1698261
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79740.46953913444399886No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC41300.24318994547952288No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG17430.10263440071932406No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGCG406.8175723E-945.0000041
GTCGACG207.0342387E-445.0000049
ACGTTAG900.045.01
ACTACGC253.891596E-545.031
TAATCGC253.891596E-545.025
GTATCGA302.1661162E-644.99999613
CGTTGAT2850.044.21052625
CGAATGG1550.043.5483862
TTTACGG1600.042.1875042
TCGTTGA3000.042.024
GCGAGAC4800.041.71874621
ATGACGG1700.041.029412
CACGACC4950.040.9090927
TTAATCG3100.040.6451620
CACAACG2950.040.4237312
AAACACG4750.040.26315740
ACACGAC5000.040.0526
ATACGAG451.9292202E-840.01
ACGGGAT5150.039.7572825
GGTATGC5500.039.681828
Produced by FastQC (version 0.11.5)