##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547586_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1698261
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.280201335366	31.0	31.0	33.0	30.0	34.0
2	31.684351227520388	31.0	31.0	34.0	30.0	34.0
3	31.835064221577248	31.0	31.0	34.0	30.0	34.0
4	35.598646497799805	37.0	35.0	37.0	33.0	37.0
5	35.50383951583414	37.0	35.0	37.0	33.0	37.0
6	35.542059789396326	37.0	35.0	37.0	33.0	37.0
7	35.87599668131106	37.0	35.0	37.0	35.0	37.0
8	35.88935328550794	37.0	35.0	37.0	35.0	37.0
9	37.6986311291374	39.0	37.0	39.0	35.0	39.0
10	37.08912057687246	39.0	37.0	39.0	33.0	39.0
11	36.773150887878835	39.0	35.0	39.0	32.0	39.0
12	36.015344520070826	37.0	35.0	39.0	32.0	39.0
13	35.72389579693581	37.0	35.0	39.0	31.0	39.0
14	36.71693338067588	38.0	35.0	41.0	31.0	41.0
15	36.99413046640063	38.0	35.0	41.0	32.0	41.0
16	37.11044592085669	38.0	35.0	41.0	32.0	41.0
17	36.99962726577363	38.0	35.0	41.0	32.0	41.0
18	36.93467435217555	38.0	35.0	41.0	32.0	41.0
19	36.88450244102644	38.0	35.0	41.0	32.0	41.0
20	36.73307224272359	38.0	35.0	40.0	31.0	41.0
21	36.534419032174675	38.0	35.0	40.0	31.0	41.0
22	36.44098639726167	38.0	35.0	40.0	31.0	41.0
23	36.37790009898361	38.0	35.0	40.0	31.0	41.0
24	36.293033874062935	38.0	35.0	40.0	31.0	41.0
25	36.18960630904201	38.0	35.0	40.0	31.0	41.0
26	36.032643392270096	37.0	35.0	40.0	30.0	41.0
27	35.95433328563748	37.0	35.0	40.0	30.0	41.0
28	35.97443914686847	37.0	35.0	40.0	30.0	41.0
29	36.02812877408125	37.0	35.0	40.0	30.0	41.0
30	36.04954244371154	38.0	35.0	40.0	30.0	41.0
31	35.95780036166408	38.0	35.0	40.0	30.0	41.0
32	35.81444018322272	38.0	34.0	40.0	30.0	41.0
33	35.69108046407472	38.0	34.0	40.0	29.0	41.0
34	35.627424170960765	38.0	34.0	40.0	29.0	41.0
35	35.48432720294466	37.0	34.0	40.0	29.0	41.0
36	35.352865666702584	37.0	34.0	40.0	27.0	41.0
37	35.31116124082223	37.0	34.0	40.0	27.0	41.0
38	35.217047909596936	37.0	34.0	40.0	27.0	41.0
39	35.17291982798875	37.0	34.0	40.0	27.0	41.0
40	35.05441978588686	37.0	34.0	40.0	27.0	41.0
41	34.99384959084617	37.0	34.0	40.0	26.0	41.0
42	34.96190927071869	37.0	34.0	40.0	26.0	41.0
43	34.909037538988414	37.0	34.0	40.0	26.0	41.0
44	34.78157126613636	36.0	34.0	40.0	26.0	41.0
45	34.683696440064274	36.0	34.0	40.0	26.0	41.0
46	34.651002996594755	36.0	34.0	40.0	26.0	41.0
47	34.59789278562011	36.0	34.0	40.0	26.0	41.0
48	34.45854494686035	36.0	34.0	40.0	25.0	41.0
49	34.40396558597295	36.0	34.0	40.0	24.0	41.0
50	34.300151154622284	35.0	33.0	40.0	24.0	41.0
51	33.87174174052163	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	4.0
12	3.0
13	9.0
14	12.0
15	42.0
16	113.0
17	335.0
18	750.0
19	1410.0
20	2477.0
21	4089.0
22	6251.0
23	9317.0
24	13322.0
25	18253.0
26	23872.0
27	29080.0
28	33261.0
29	38347.0
30	45058.0
31	54612.0
32	67731.0
33	87858.0
34	151930.0
35	234418.0
36	120713.0
37	150320.0
38	224679.0
39	379852.0
40	141.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.54445459207978	23.562514831348068	28.790745356573577	13.102285219998574
2	30.498256746165637	26.67740706522731	29.30409401146231	13.520242177144738
3	29.446357185379636	26.026505937544346	30.79149789107799	13.73563898599803
4	26.15746342876625	28.904567672460242	30.183758562435337	14.754210336338172
5	24.02551786798378	33.14213775149992	28.678454018551918	14.153890361964386
6	22.861032550355922	41.652902586822634	25.27938873942227	10.20667612339917
7	88.82851340282795	3.872372974472122	5.890319568075815	1.4087940546241124
8	89.16103001835407	3.2757626772327693	5.575409197997246	1.987798106415916
9	83.77375444646023	6.130329790297251	7.404692211621182	2.6912235516213348
10	45.60794836600499	32.0530236518415	12.42735951658785	9.911668465565658
11	41.40005570404078	21.895456587650543	22.482763250171793	14.221724458136883
12	38.82259558454207	21.032220606844295	24.430755932097597	15.714427876516035
13	22.591403794823055	35.136354188196044	25.47123204266011	16.80100997432079
14	16.857126201449603	37.77116709386837	27.241042454605036	18.130664250076993
15	16.19698032281257	26.125018474780966	41.92747757853475	15.750523623871713
16	18.975705147795306	22.010692113874132	41.39622825937827	17.617374478952293
17	18.697656014004917	22.41875659866181	28.767545153542358	30.116042233790917
18	22.303933258786486	25.017768175798654	32.26206101417862	20.416237551236236
19	28.044511414912076	26.604096778999224	25.919631905814246	19.431759900274457
20	31.377980180902696	24.37805496328303	25.950663649462598	18.29330120635167
21	24.307747749020912	27.95524362862952	27.582862704849255	20.154145917500312
22	24.121027333254432	24.487343229338716	26.579660016923196	24.81196942048366
23	21.295725450917143	29.29137511842997	26.10641120534476	23.306488225308126
24	20.924698853709767	25.480888979962447	35.674257372688885	17.920154793638904
25	19.39213112707646	26.653029186915322	32.98179726202274	20.973042423985476
26	19.711163360637734	33.648479238468056	26.70949871662836	19.930858684265846
27	20.181409100250196	33.366602660015154	28.140491950295036	18.311496289439606
28	17.047438526822436	30.34992854455234	34.72293128088085	17.87970164774437
29	17.951422072343416	25.84561501441769	34.19038651891553	22.012576394323368
30	19.5293891810505	30.773361691754094	30.366062695898922	19.331186431296484
31	27.807327613364496	27.767875491458614	25.11704620196778	19.307750693209112
32	28.756298354610983	27.589222151365426	26.05465237675481	17.59982711726878
33	26.174009766461104	29.65174375434636	24.397251070359623	19.776995408832917
34	20.224806434346664	29.062022857499525	27.37070450301809	23.34246620513572
35	21.130792027844954	26.953925221152698	29.459959334872554	22.455323416129794
36	27.336434152347604	27.366641523299425	26.403067608571355	18.893856715781617
37	21.182668623962982	31.543561325379315	28.36183601931623	18.911934031341474
38	20.842026048999536	31.583602284925576	25.132297096853783	22.442074569221106
39	21.555461734091523	30.9514261942069	27.237215009942524	20.25589706175906
40	24.233318671276088	27.60800607209375	26.681587812474056	21.47708744415611
41	18.52347783997866	25.77201030936941	28.899915855101188	26.804595995550745
42	22.19511606284311	26.09027705399818	25.6602489252241	26.054357957934617
43	22.432535399446845	25.9377092213741	27.15978286023173	24.469972518947323
44	20.119816683065793	29.628896853899374	28.343287633644067	21.90799882939077
45	18.824609409272192	34.347135098786346	24.47279893962118	22.355456552320284
46	21.857476559845633	33.0110036089859	24.94993407962616	20.181585751542315
47	21.80259689176163	28.59866651827958	27.14818275871612	22.450553831242665
48	23.204972616105536	25.051037502480476	31.163819931094217	20.580169950319767
49	21.287952794064047	25.776721010492498	30.441080611284132	22.494245584159327
50	20.201665115079486	31.366144544330936	28.24995686764284	20.182233472946738
51	19.095239188793713	32.333840322541704	25.85974711778696	22.71117337087762
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1016.0
1	1947.5
2	2879.0
3	8280.0
4	13681.0
5	9958.0
6	6235.0
7	6276.0
8	6317.0
9	6681.0
10	7045.0
11	7219.5
12	7394.0
13	7446.0
14	7498.0
15	7100.5
16	6703.0
17	6686.5
18	6670.0
19	6486.0
20	6302.0
21	6712.5
22	7123.0
23	7194.5
24	7266.0
25	8934.0
26	12742.0
27	14882.0
28	19806.5
29	24731.0
30	27550.0
31	30369.0
32	34892.5
33	39416.0
34	43596.0
35	47776.0
36	50118.0
37	52460.0
38	61778.0
39	71096.0
40	92256.0
41	113416.0
42	135010.0
43	156604.0
44	159820.5
45	163037.0
46	152642.5
47	142248.0
48	138959.5
49	135671.0
50	129870.0
51	124069.0
52	115907.5
53	107746.0
54	97269.0
55	86792.0
56	78851.0
57	70910.0
58	65133.5
59	59357.0
60	53663.5
61	47970.0
62	41650.0
63	35330.0
64	29171.5
65	23013.0
66	20112.5
67	17212.0
68	14723.5
69	12235.0
70	10622.5
71	9010.0
72	7710.0
73	6410.0
74	5307.5
75	3555.0
76	2905.0
77	2142.5
78	1380.0
79	1078.5
80	777.0
81	543.0
82	309.0
83	212.5
84	116.0
85	68.5
86	21.0
87	31.5
88	42.0
89	25.5
90	9.0
91	6.0
92	3.0
93	1.5
94	0.0
95	0.5
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1698261.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.01872690307651
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.87021424092346	14.211408511943358
2	7.253083428212139	2.613826589963689
3	2.4338123181727593	1.3156259848349552
4	1.2171611801068658	0.8772677960548774
5	0.6959591976281025	0.6270149358872515
6	0.519912099074291	0.5620892476094944
7	0.4145963472938697	0.5229348849830913
8	0.32156550545982093	0.4635360819464217
9	0.26776649438450667	0.43423302024977356
>10	4.845309444810769	22.81246843371513
>50	2.053077759734754	26.569365661153306
>100	1.0995907405190628	27.24411002426229
>500	0.006294734579649533	0.7768288649883559
>1k	0.0013252072799262175	0.4932711066932134
>5k	3.313018199815544E-4	0.4760188557148352
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7974	0.46953913444399886	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4130	0.24318994547952288	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1743	0.10263440071932406	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.029500765783351323	0.0
2	0.0	0.0	0.0	0.09680490807950015	0.0
3	0.0	0.0	0.0	0.1532155540285033	0.0
4	0.0	0.0	0.0	0.35948537945580805	0.0
5	0.0	0.0	0.0	0.5950793193743482	0.0
6	0.0	0.0	0.0	1.0037326418024084	0.0
7	0.0	0.0	0.0	1.1614822456618858	0.0
8	0.0	0.0	0.0	1.605230291456967	0.0
9	0.0	0.0	0.0	1.743077183071389	0.0
10	0.0	0.0	0.0	1.993804250347856	0.0
11	0.0	0.0	0.0	2.3102455982914285	0.0
12	0.0	0.0	0.0	2.5716895106229254	0.0
13	0.0	0.0	0.0	2.6969352767330816	0.0
14	0.0	0.0	0.0	2.7471042436939905	0.0
15	0.0	0.0	0.0	2.824300858348628	0.0
16	0.0	0.0	0.0	2.974454456647123	0.0
17	0.0	0.0	0.0	3.134971597416416	0.0
18	0.0	0.0	0.0	3.339003839810253	0.0
19	0.0	0.0	0.0	3.4558881114269244	0.0
20	0.0	0.0	0.0	3.5836658793907414	0.0
21	0.0	0.0	0.0	3.7576085183608408	0.0
22	0.0	0.0	0.0	3.9396771167682707	0.0
23	0.0	0.0	0.0	4.139823030735558	0.0
24	0.0	0.0	0.0	4.29439291133695	0.0
25	0.0	0.0	0.0	4.42417272727808	0.0
26	0.0	0.0	0.0	4.552362681590168	0.0
27	0.0	0.0	0.0	4.669305836970878	0.0
28	0.0	0.0	0.0	4.8115690108881966	0.0
29	0.0	0.0	0.0	4.952890044580898	0.0
30	0.0	0.0	0.0	5.143084602425658	0.0
31	0.0	0.0	0.0	5.317557195272105	0.0
32	0.0	0.0	0.0	5.483256107276796	0.0
33	0.0	0.0	0.0	5.642124502653008	0.0
34	0.0	0.0	0.0	5.803524899882881	0.0
35	0.0	0.0	0.0	5.997252483569958	0.0
36	0.0	0.0	0.0	6.184620620740864	0.0
37	0.0	0.0	0.0	6.376758342798898	0.0
38	0.0	0.0	0.0	6.567247319463852	0.0
39	0.0	0.0	0.0	6.766568860734599	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGCG	40	6.8175723E-9	45.000004	1
GTCGACG	20	7.0342387E-4	45.000004	9
ACGTTAG	90	0.0	45.0	1
ACTACGC	25	3.891596E-5	45.0	31
TAATCGC	25	3.891596E-5	45.0	25
GTATCGA	30	2.1661162E-6	44.999996	13
CGTTGAT	285	0.0	44.210526	25
CGAATGG	155	0.0	43.548386	2
TTTACGG	160	0.0	42.187504	2
TCGTTGA	300	0.0	42.0	24
GCGAGAC	480	0.0	41.718746	21
ATGACGG	170	0.0	41.02941	2
CACGACC	495	0.0	40.90909	27
TTAATCG	310	0.0	40.64516	20
CACAACG	295	0.0	40.42373	12
AAACACG	475	0.0	40.263157	40
ACACGAC	500	0.0	40.05	26
ATACGAG	45	1.9292202E-8	40.0	1
ACGGGAT	515	0.0	39.757282	5
GGTATGC	550	0.0	39.68182	8
>>END_MODULE