FastQCFastQC Report
Thu 26 May 2016
SRR1547585_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547585_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2865428
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT435701.5205407359738232No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC33030.11527073791419641No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTT31720.11069899505414199No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAG600.045.0000041
TATCACG207.0350524E-445.01
TAGGTCG207.0350524E-445.01
ACGTCCG253.89227E-545.025
CGTTGAT3150.044.28571325
CGTTTTT239350.043.7027321
TCGTTGA3300.042.27272824
CGTAAGG4900.041.7857132
TACGAAT3150.041.42857412
CGTTAGG4050.041.111112
GCGTTAG2050.040.6097531
CGCTAGG2550.040.5882342
TTCGCGA501.0822987E-940.515
CTAACGT451.9299478E-840.022
CGAATAT3300.039.54545214
GCTACGA3250.039.4615410
TCCGAAC403.4606273E-739.37517
TCGTAAG2300.039.1304321
AGTACGG3800.039.0789452
GTAGGGC15100.038.8907284
Produced by FastQC (version 0.11.5)