FastQCFastQC Report
Thu 26 May 2016
SRR1547584_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547584_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences238549
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10310.43219632025286214No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC4250.17816046179191697No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT2970.12450272271105726No Hit
GAGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT2660.11150748902741157No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTAC650.045.00000426
CTTGTCT453.8198777E-1045.039
TTGATCG207.022036E-445.034
GATCGTA207.022036E-445.036
AGGAGTA207.022036E-445.010
TATGGCC502.1827873E-1145.010
GGACTAA1000.045.08
TAGCGAG351.2065175E-745.01
CTCCCAA253.8814826E-545.034
GTTAGCG207.022036E-445.01
AATGGGC253.8814826E-545.04
ATATGCT502.1827873E-1145.034
GTAGGCC207.022036E-445.022
TCGATAG253.8814826E-545.01
CGGGGTA207.022036E-445.06
TGTTGCG207.022036E-445.01
CTAACGG253.8814826E-545.02
TGCGCGG253.8814826E-545.02
ATTCATA207.022036E-445.043
GCGATGC253.8814826E-545.09