FastQCFastQC Report
Thu 26 May 2016
SRR1547583_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547583_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2302039
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159900.6946016118753853No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC54850.23826703196600926No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACGA302.1664546E-645.00000416
TAGTACG650.045.0000041
AACCGGT502.1827873E-1145.03
CGCGCTA207.034763E-445.040
CGCGATA207.034763E-445.011
TAACGCG207.034763E-445.01
ATCGTAC253.892029E-545.041
TACGCTA207.034763E-445.039
TACGAAC253.892029E-545.034
TTGCGAC207.034763E-445.019
ACGCTAG502.1827873E-1145.01
GTATCCG253.892029E-545.01
TCGTACC207.034763E-445.030
CCGACAA351.2126475E-745.030
CCGCGAT207.034763E-445.010
GCCCGTT207.034763E-445.023
TGGTCGC207.034763E-445.045
CGCTTAA207.034763E-445.029
CGGACTA207.034763E-445.034
TACGCGG1700.043.676472