##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547583_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2302039
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.244940680848586	31.0	31.0	33.0	30.0	34.0
2	31.63677200950983	31.0	31.0	34.0	30.0	34.0
3	31.694893526999326	31.0	31.0	34.0	30.0	34.0
4	35.506255106885675	37.0	35.0	37.0	33.0	37.0
5	35.43132023393174	37.0	35.0	37.0	33.0	37.0
6	35.48305306730251	37.0	35.0	37.0	33.0	37.0
7	35.88621261412165	37.0	35.0	37.0	35.0	37.0
8	35.922449185265755	37.0	35.0	37.0	35.0	37.0
9	37.76946263725332	39.0	38.0	39.0	35.0	39.0
10	36.991845490019934	39.0	37.0	39.0	33.0	39.0
11	36.68985451593131	39.0	35.0	39.0	32.0	39.0
12	35.816002682839	37.0	35.0	39.0	31.0	39.0
13	35.505715585183395	37.0	35.0	39.0	30.0	39.0
14	36.456868454444084	38.0	35.0	41.0	31.0	41.0
15	36.7686090461543	38.0	35.0	41.0	32.0	41.0
16	36.90980257067756	38.0	35.0	41.0	32.0	41.0
17	36.826608932342154	38.0	35.0	41.0	32.0	41.0
18	36.745204577333396	38.0	35.0	40.0	32.0	41.0
19	36.64232143764723	37.0	35.0	40.0	32.0	41.0
20	36.40731021498767	37.0	35.0	40.0	31.0	41.0
21	36.220818587348	37.0	35.0	40.0	31.0	41.0
22	36.14255883588419	37.0	35.0	40.0	31.0	41.0
23	36.10354950546016	37.0	35.0	40.0	31.0	41.0
24	36.025875756231756	37.0	35.0	40.0	31.0	41.0
25	35.93535035679239	36.0	35.0	40.0	31.0	41.0
26	35.78436898766702	36.0	34.0	40.0	30.0	41.0
27	35.716953101142074	36.0	34.0	40.0	30.0	41.0
28	35.73677726571965	36.0	35.0	40.0	30.0	41.0
29	35.77269542349195	36.0	35.0	40.0	30.0	41.0
30	35.728067161329584	36.0	35.0	40.0	30.0	41.0
31	35.5405964017117	36.0	34.0	40.0	30.0	41.0
32	35.320190926391774	36.0	34.0	40.0	29.0	41.0
33	35.1094130029943	36.0	34.0	40.0	27.0	41.0
34	34.963931540690666	36.0	34.0	40.0	27.0	41.0
35	34.74354300687347	36.0	34.0	40.0	25.0	41.0
36	34.55674512899217	36.0	34.0	40.0	24.0	41.0
37	34.47996797621587	36.0	34.0	40.0	24.0	41.0
38	34.41210726664492	36.0	33.0	40.0	24.0	41.0
39	34.38177893597806	36.0	34.0	40.0	23.0	41.0
40	34.274528798165456	36.0	34.0	40.0	23.0	41.0
41	34.235990789035284	36.0	34.0	40.0	23.0	41.0
42	34.187942080911746	36.0	33.0	40.0	23.0	41.0
43	34.131818357551715	35.0	33.0	40.0	23.0	41.0
44	33.998679431582175	35.0	33.0	40.0	23.0	41.0
45	33.90631435870548	35.0	33.0	40.0	23.0	41.0
46	33.88932854743121	35.0	33.0	40.0	23.0	41.0
47	33.8441564195915	35.0	33.0	40.0	23.0	41.0
48	33.7179769760634	35.0	33.0	40.0	23.0	41.0
49	33.68817643836616	36.0	33.0	40.0	22.0	41.0
50	33.55779028939128	35.0	33.0	40.0	22.0	41.0
51	33.14105495171889	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	3.0
13	8.0
14	6.0
15	63.0
16	171.0
17	517.0
18	1236.0
19	2514.0
20	4164.0
21	6736.0
22	10741.0
23	15949.0
24	23595.0
25	34546.0
26	45003.0
27	49596.0
28	51152.0
29	55449.0
30	63764.0
31	75878.0
32	94372.0
33	122441.0
34	214479.0
35	360250.0
36	150805.0
37	178778.0
38	265930.0
39	473722.0
40	170.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.341041572275707	24.3322984536752	28.800684957987244	15.525975016061846
2	31.4274866759425	25.82028367025928	29.61361645045979	13.138613203338432
3	27.809780807362515	24.138687485311934	34.02414120699084	14.027390500334702
4	22.973633374586615	28.196307708079665	33.93647979030764	14.893579127026085
5	22.20049269365115	31.512672026842292	33.07680712620421	13.210028153302355
6	19.95161680579695	40.33936870748063	29.41587870579082	10.2931357809316
7	86.4765106064667	3.6676181420036755	8.589819720691091	1.2660515308385305
8	87.5425220858552	2.6023451383751532	7.8023439220621364	2.0527888537075176
9	83.93780470270052	4.286591148108264	9.707741702030244	2.0678624471609734
10	48.799260134168016	19.613655546235314	19.224305061730057	12.362779257866613
11	43.17168388545981	22.810777749638472	20.33345221345077	13.684086151450952
12	37.40992224719042	20.9310094225163	26.502678712219907	15.156389618073367
13	19.525168774290965	40.3480132178473	27.40944006595892	12.717377941902807
14	15.253781538887917	41.793427478856785	29.41392391701444	13.53886706524086
15	14.272477573142767	25.353002273202147	47.53598874736701	12.838531406288078
16	14.490892639090823	20.328717280636862	47.82508028751902	17.355309792753292
17	15.113905541999939	22.518515107693656	30.00930913855065	32.35827021175575
18	20.268422906823037	24.49506719912217	36.13548684448873	19.10102304956606
19	26.768269347304713	27.472731782563198	27.2223450601836	18.536653809948483
20	28.170156978226697	24.295418105427405	29.1735283372697	18.360896579076204
21	19.614089943741178	29.74845343627975	30.96294198317231	19.674514636806762
22	21.10146700381705	25.35321947195508	28.02350438024725	25.52180914398062
23	17.381026125100398	31.606979725365207	27.21700197086148	23.794992178672906
24	18.382486135117606	25.45647575909878	39.71170775125877	16.449330354524836
25	15.721106375695634	27.010315637571736	37.068616126833646	20.199961859898984
26	15.497956376933667	35.32524861655254	29.884593614617298	19.292201391896487
27	16.7411151592132	35.779324329431425	30.163911210887388	17.315649300467975
28	14.62877040745183	30.03485171189541	39.30237498148381	16.034002899168954
29	14.4436301904529	26.12466600261768	38.431190783475	21.000513023454424
30	16.76339975126399	32.41031103295817	33.11816176876239	17.70812744701545
31	26.185221014935024	28.22706305149478	28.22710649124537	17.360609442324826
32	26.219842496152324	27.517691924420046	29.502714767212897	16.759750812214737
33	25.304653830799566	29.35436801896058	26.79541919142117	18.54555895881868
34	17.685017499703523	29.925122901914346	29.04989880710101	23.33996079128112
35	18.679396830375158	28.025850126778913	31.75649934688335	21.538253695962577
36	27.845748920847996	26.221753845178124	27.995746379622588	17.936750854351295
37	18.83008932515913	32.762129572956844	31.614234163713125	16.7935469381709
38	19.364441697121553	33.2486982192743	26.130052531690385	21.25680755191376
39	19.584550913342476	32.87398693071664	28.657768178558225	18.88369397738266
40	23.13853066781232	28.20760204323211	26.34481865858919	22.309048630366384
41	16.267795636824573	25.59344129269747	30.3163847354454	27.82237833503255
42	20.983224002721066	25.451610507033113	27.457527869857984	26.10763762038784
43	21.001077740212047	26.745550357748066	28.75754928565502	23.495822616384864
44	18.09969335880061	31.307115127067785	29.451108343516335	21.142083170615265
45	16.41001738024421	38.68496580640032	24.350803787424972	20.55421302593049
46	21.933511986547575	34.09490456069598	25.354913622227947	18.6166698305285
47	20.077852721000816	29.088907703127532	27.92211600237876	22.91112357349289
48	22.06326652154894	25.68727115396394	32.20805555422823	20.041406770258888
49	20.97323286008621	25.265253977017764	31.832171392404735	21.92934177049129
50	19.165140121431477	32.757307760641766	28.173284640268907	19.90426747765785
51	17.43341446430751	33.625147097855425	25.869935305179453	23.071503132657615
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1218.0
1	1734.0
2	2250.0
3	13309.0
4	24368.0
5	18265.5
6	12163.0
7	12840.5
8	13518.0
9	15412.0
10	17306.0
11	18512.5
12	19719.0
13	19636.5
14	19554.0
15	18664.5
16	17775.0
17	16517.0
18	15259.0
19	14366.5
20	13474.0
21	13010.5
22	12547.0
23	13654.0
24	14761.0
25	15784.0
26	19338.0
27	21869.0
28	24594.0
29	27319.0
30	32056.5
31	36794.0
32	42878.0
33	48962.0
34	58615.0
35	68268.0
36	70217.0
37	72166.0
38	88965.5
39	105765.0
40	139274.0
41	172783.0
42	211605.5
43	250428.0
44	249341.0
45	248254.0
46	244989.0
47	241724.0
48	227518.5
49	213313.0
50	191526.0
51	169739.0
52	148892.5
53	128046.0
54	112606.5
55	97167.0
56	81669.5
57	66172.0
58	56075.5
59	45979.0
60	39800.5
61	33622.0
62	26917.5
63	20213.0
64	16692.0
65	13171.0
66	10291.0
67	7411.0
68	6206.0
69	5001.0
70	3868.0
71	2735.0
72	2364.5
73	1994.0
74	1573.5
75	919.0
76	685.0
77	532.0
78	379.0
79	235.0
80	91.0
81	78.0
82	65.0
83	48.0
84	31.0
85	21.0
86	11.0
87	8.5
88	6.0
89	4.0
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2302039.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.25611797788348
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.09369145247214	16.0213114536759
2	8.613436927922354	3.4894958921410693
3	3.1687525285567744	1.9255987518348783
4	1.5531036564261425	1.2583940358580061
5	0.9321725514257198	0.9441098588712017
6	0.6200581374777014	0.7535982471537008
7	0.4114128358608454	0.5833538858569028
8	0.3294696673885657	0.5339021162205453
9	0.27210176650618134	0.4960552935905741
>10	2.6899426925570498	12.705657295531402
>50	1.0757960847211603	15.936348075976852
>100	1.2302665827247528	42.941922058933336
>500	0.007618423524936922	0.9952737902590457
>1k	0.0017413539485570109	0.4681177438169622
>5k	2.1766924356962636E-4	0.24184099320297991
>10k+	2.1766924356962636E-4	0.7050205070766907
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15990	0.6946016118753853	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5485	0.23826703196600926	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.343975058632803E-5	0.0	0.0	0.04882627965903271	0.0
2	4.343975058632803E-5	0.0	0.0	0.16824215402084847	0.0
3	4.343975058632803E-5	0.0	0.0	0.2682404598705756	0.0
4	4.343975058632803E-5	0.0	0.0	0.5513807541922617	0.0
5	4.343975058632803E-5	0.0	0.0	0.9123650815646477	0.0
6	4.343975058632803E-5	0.0	0.0	1.5436315370851668	0.0
7	4.343975058632803E-5	0.0	0.0	1.7872416583732942	0.0
8	4.343975058632803E-5	0.0	0.0	2.466986875548155	0.0
9	4.343975058632803E-5	0.0	0.0	2.691526946328885	0.0
10	4.343975058632803E-5	0.0	0.0	3.0984270900710196	0.0
11	4.343975058632803E-5	0.0	0.0	3.5865161276590016	0.0
12	4.343975058632803E-5	0.0	0.0	3.9631822049930516	0.0
13	4.343975058632803E-5	0.0	0.0	4.144499723940385	0.0
14	4.343975058632803E-5	0.0	0.0	4.211483819344503	0.0
15	4.343975058632803E-5	0.0	0.0	4.299623073284162	0.0
16	4.343975058632803E-5	0.0	0.0	4.517169344220493	0.0
17	4.343975058632803E-5	0.0	0.0	4.7745064266939	0.0
18	4.343975058632803E-5	0.0	0.0	5.095091786021001	0.0
19	4.343975058632803E-5	0.0	0.0	5.2721956491614606	0.0
20	4.343975058632803E-5	0.0	0.0	5.447127524772604	0.0
21	4.343975058632803E-5	0.0	0.0	5.701727902959072	0.0
22	4.343975058632803E-5	0.0	0.0	5.967666056048572	0.0
23	4.343975058632803E-5	0.0	0.0	6.304454442344374	0.0
24	4.343975058632803E-5	0.0	0.0	6.5268225255957875	0.0
25	4.343975058632803E-5	0.0	0.0	6.722648921238954	0.0
26	4.343975058632803E-5	0.0	0.0	6.912524071051793	0.0
27	4.343975058632803E-5	0.0	0.0	7.100618191090594	0.0
28	4.343975058632803E-5	0.0	0.0	7.304871898347509	0.0
29	4.343975058632803E-5	0.0	0.0	7.525111433820191	0.0
30	4.343975058632803E-5	0.0	0.0	7.780015890260764	0.0
31	4.343975058632803E-5	0.0	0.0	8.030141974136841	0.0
32	4.343975058632803E-5	0.0	0.0	8.260937369002002	0.0
33	4.343975058632803E-5	0.0	0.0	8.499595358723289	0.0
34	4.343975058632803E-5	0.0	0.0	8.740685974477408	0.0
35	4.343975058632803E-5	0.0	0.0	9.0180922217217	0.0
36	4.343975058632803E-5	0.0	0.0	9.267392950336637	0.0
37	4.343975058632803E-5	0.0	0.0	9.524208755803008	0.0
38	4.343975058632803E-5	0.0	0.0	9.776376507956641	0.0
39	4.343975058632803E-5	0.0	0.0	10.035538059954675	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTACGA	30	2.1664546E-6	45.000004	16
TAGTACG	65	0.0	45.000004	1
AACCGGT	50	2.1827873E-11	45.0	3
CGCGCTA	20	7.034763E-4	45.0	40
CGCGATA	20	7.034763E-4	45.0	11
TAACGCG	20	7.034763E-4	45.0	1
ATCGTAC	25	3.892029E-5	45.0	41
TACGCTA	20	7.034763E-4	45.0	39
TACGAAC	25	3.892029E-5	45.0	34
TTGCGAC	20	7.034763E-4	45.0	19
ACGCTAG	50	2.1827873E-11	45.0	1
GTATCCG	25	3.892029E-5	45.0	1
TCGTACC	20	7.034763E-4	45.0	30
CCGACAA	35	1.2126475E-7	45.0	30
CCGCGAT	20	7.034763E-4	45.0	10
GCCCGTT	20	7.034763E-4	45.0	23
TGGTCGC	20	7.034763E-4	45.0	45
CGCTTAA	20	7.034763E-4	45.0	29
CGGACTA	20	7.034763E-4	45.0	34
TACGCGG	170	0.0	43.67647	2
>>END_MODULE