FastQCFastQC Report
Thu 26 May 2016
SRR1547582_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547582_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2642905
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT752462.84709439045293No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTT59210.22403378100991142No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCT44830.16962395545810385No Hit
CGTTCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTC32690.12368965210629969No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32060.12130591148754874No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT322350.043.7087021
ATAACGC800.042.187511
TCGATAG650.041.538461
TACGGGA10850.040.8525354
TATAGCG1550.040.645161
CGGTTTT11450.039.4978181
CATACGA11000.039.27272818
GCGTAAG1550.039.1935461
CGACGGT1500.039.00000428
ATAGCGG3950.038.7341772
ACGGGAT11450.038.5152855
GTTTTTT374750.038.4676442
CTATGCG1000.038.251
CACGACC11950.038.2217627
GCGAGAC11900.038.19327521
CGTAAGG3950.038.164562
CGTTCGA1250.037.80000314
GGGCGAT43350.037.6297577
ACACGAC12100.037.5619826
TAACGCG601.5643309E-1037.5000041