##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547582_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2642905
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.36442475230854	31.0	31.0	33.0	30.0	34.0
2	31.74989906939523	31.0	31.0	34.0	30.0	34.0
3	31.606788363562064	31.0	31.0	34.0	30.0	34.0
4	35.57569606171997	37.0	35.0	37.0	33.0	37.0
5	35.57487007667699	37.0	35.0	37.0	33.0	37.0
6	35.64071353302521	37.0	35.0	37.0	33.0	37.0
7	35.941781486659565	37.0	35.0	37.0	35.0	37.0
8	35.975641576220106	37.0	35.0	37.0	35.0	37.0
9	37.81494113484972	39.0	38.0	39.0	35.0	39.0
10	37.26396522008926	39.0	37.0	39.0	34.0	39.0
11	37.00419689697511	39.0	37.0	39.0	33.0	39.0
12	36.6073581910814	39.0	35.0	39.0	32.0	39.0
13	36.50507339461691	39.0	35.0	39.0	32.0	39.0
14	37.64079601801805	40.0	36.0	41.0	33.0	41.0
15	37.77737489618431	40.0	36.0	41.0	33.0	41.0
16	37.81965564407347	40.0	36.0	41.0	33.0	41.0
17	37.70201728779506	40.0	36.0	41.0	33.0	41.0
18	37.48988177781646	39.0	36.0	41.0	33.0	41.0
19	37.304469135288635	38.0	36.0	41.0	33.0	41.0
20	37.02170187729033	38.0	35.0	41.0	32.0	41.0
21	36.8610926234579	38.0	35.0	41.0	32.0	41.0
22	36.80665858212838	38.0	35.0	41.0	32.0	41.0
23	36.70169680711187	38.0	35.0	40.0	32.0	41.0
24	36.568066199882324	38.0	35.0	40.0	31.0	41.0
25	36.45007709319858	38.0	35.0	40.0	31.0	41.0
26	36.32929976673395	38.0	35.0	40.0	31.0	41.0
27	36.247979779825606	38.0	35.0	40.0	31.0	41.0
28	36.15414137095355	37.0	35.0	40.0	31.0	41.0
29	36.088072026803836	37.0	35.0	40.0	31.0	41.0
30	35.94508769706062	37.0	35.0	40.0	30.0	41.0
31	35.74691485316347	37.0	35.0	40.0	30.0	41.0
32	35.42439323396036	37.0	34.0	40.0	28.0	41.0
33	35.0734937502483	37.0	34.0	40.0	25.0	41.0
34	34.72583653214928	37.0	34.0	40.0	23.0	41.0
35	34.35807832668976	37.0	34.0	40.0	21.0	41.0
36	34.179984524604556	37.0	33.0	40.0	21.0	41.0
37	34.09581729195715	37.0	33.0	40.0	20.0	41.0
38	33.94983361112109	37.0	33.0	40.0	19.0	41.0
39	33.844445033022375	36.0	33.0	40.0	18.0	41.0
40	33.746842205830326	36.0	33.0	40.0	18.0	41.0
41	33.617780434786724	36.0	33.0	40.0	18.0	41.0
42	33.54684674628865	36.0	33.0	40.0	18.0	41.0
43	33.47274987182664	36.0	33.0	40.0	18.0	41.0
44	33.332203768202035	36.0	33.0	40.0	17.0	41.0
45	33.23624572203693	35.0	33.0	40.0	17.0	41.0
46	33.19999886488542	35.0	33.0	40.0	18.0	41.0
47	33.14442214154501	35.0	33.0	40.0	17.0	41.0
48	32.980492677565024	35.0	32.0	40.0	15.0	41.0
49	32.8803403830255	35.0	32.0	40.0	15.0	41.0
50	32.80943772099262	35.0	32.0	40.0	15.0	41.0
51	32.46253535408953	35.0	31.0	39.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	3.0
11	5.0
12	1.0
13	9.0
14	29.0
15	62.0
16	181.0
17	522.0
18	1238.0
19	2543.0
20	4731.0
21	7806.0
22	12208.0
23	19002.0
24	29803.0
25	46719.0
26	65017.0
27	71409.0
28	68282.0
29	65110.0
30	69577.0
31	79517.0
32	95283.0
33	121012.0
34	188609.0
35	291608.0
36	234968.0
37	248350.0
38	338699.0
39	580414.0
40	185.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.61112677148819	21.8139887737168	25.674399950054962	21.90048450474005
2	38.416098951721686	23.261297700825416	25.816932504195194	12.505670843257702
3	26.942398610619755	23.025912774011932	37.510693725275786	12.52099489009253
4	22.73116135464574	26.038393358822965	37.63374014578655	13.59670514074475
5	21.442844143092543	29.47200145294666	36.02025044411358	13.064903959847213
6	19.815354694928498	37.437932880674865	32.88506397316589	9.861648451230748
7	80.58571912346451	2.7013835154876924	15.113974963156075	1.5989223978917138
8	80.8110393676655	2.4694039324152777	14.92528108274796	1.7942756171712566
9	76.14953999481631	4.9194352426591195	16.432978105531603	2.498046656992968
10	36.068946859610925	32.16997962469328	21.747735919376595	10.013337596319202
11	28.93747599705627	23.786288194240807	32.87446957041589	14.401766238287037
12	27.846971419706723	22.39304855830989	33.54445203289562	16.215527989087768
13	20.37076625909747	29.37169516119573	34.387955677559354	15.869582902147448
14	16.4234431430566	31.569466174531435	34.967545182289946	17.039545500122024
15	15.469871221250859	27.232193362985047	41.34726749542643	15.950667920337658
16	18.34742451961005	26.01754508769706	40.45798089602161	15.177049496671277
17	17.94608584114828	26.726083608756273	34.34656183252898	20.981268717566465
18	19.6888272563713	26.915458557912604	36.251057075452955	17.14465711026314
19	21.504367353347924	30.230333666930896	31.436695605782273	16.828603373938904
20	23.87887570684531	27.62036471231467	32.74907724643905	15.751682334400972
21	20.239774036524203	29.735612895658374	33.63658549966798	16.38802756814944
22	20.650004445865438	26.469623387900814	33.48080994209024	19.39956222414351
23	17.892508432955402	30.334991231239865	33.03626123526953	18.736239100535208
24	17.624470043380295	27.88503559530138	38.466800736310994	16.023693625007333
25	17.784218501989287	30.28565158414699	35.03977630675336	16.89035360711036
26	17.104322705507766	32.87689114818731	32.46919582807555	17.54959031822937
27	16.680016875370093	31.72535524356721	33.440210677266116	18.15441720379658
28	15.35711650626867	31.02321120131068	37.02342687308094	16.596245419339702
29	16.417918918765526	28.18731660805061	36.85630016969963	18.538464303484233
30	17.38613381865788	31.195256734540212	35.24954548120345	16.16906396559846
31	21.038894701095952	30.321369856275577	32.03713338163877	16.602602060989707
32	20.66956625380027	30.233020104771075	32.27361558587993	16.823798055548725
33	19.825457214693678	31.865087848409228	30.57616524241318	17.73328969448391
34	17.363696387119475	31.93686492704051	32.229497465856696	18.469941219983312
35	17.63691846661155	32.479828067978225	32.33967925445674	17.543574210953476
36	21.084147935699544	32.613166194017566	28.936076022407164	17.36660984787573
37	18.45155236378152	33.966374122414535	30.045612687554033	17.536460826249904
38	18.394342588931497	33.35371494624286	29.833005726653056	18.41893673817258
39	18.616635860918194	33.06607691158025	29.35080905291715	18.966478174584406
40	21.41291495532378	31.52519670589749	28.93581116233841	18.12607717644032
41	17.624280857616903	30.403741337656857	30.75729169228557	21.214686112440667
42	19.831246299053504	30.641245145020346	28.37688074297033	21.15062781295582
43	19.387265149522968	31.205321417152714	29.17040150894565	20.237011924378667
44	18.589052576615504	32.94030621607663	28.820142986599972	19.650498220707895
45	17.46752153406952	34.98661510723995	27.3550884348851	20.190774923805435
46	19.57001859696054	33.80518785200376	27.74829212552097	18.87650142551473
47	18.171141225280515	32.63477120819704	29.81476821906198	19.379319347460463
48	19.50020905026855	30.651234153327493	31.03569746169461	18.812859334709344
49	18.845437123165606	30.457280908697058	30.43722721777741	20.260054750359927
50	17.499153393708816	32.316560754170126	31.691112620393092	18.493173231727965
51	16.958346970473777	33.057601389380245	29.47185010433595	20.512201535810025
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1658.0
1	3016.5
2	4375.0
3	53067.5
4	101760.0
5	67309.0
6	32858.0
7	32656.0
8	32454.0
9	37199.5
10	41945.0
11	46185.0
12	50425.0
13	51528.5
14	52632.0
15	49821.0
16	47010.0
17	41970.5
18	36931.0
19	33547.0
20	30163.0
21	28653.0
22	27143.0
23	26328.0
24	25513.0
25	26554.5
26	30437.5
27	33279.0
28	36615.5
29	39952.0
30	48067.0
31	56182.0
32	62963.0
33	69744.0
34	79656.5
35	89569.0
36	91850.0
37	94131.0
38	105512.5
39	116894.0
40	133756.0
41	150618.0
42	164829.5
43	179041.0
44	192005.5
45	204970.0
46	199210.5
47	193451.0
48	180764.0
49	168077.0
50	156822.0
51	145567.0
52	133505.5
53	121444.0
54	113558.0
55	105672.0
56	95293.5
57	84915.0
58	76843.5
59	68772.0
60	62531.0
61	56290.0
62	50650.0
63	45010.0
64	39473.0
65	33936.0
66	29300.0
67	24664.0
68	20227.0
69	15790.0
70	13718.5
71	11647.0
72	9937.0
73	8227.0
74	6782.5
75	4517.5
76	3697.0
77	2771.0
78	1845.0
79	1395.5
80	946.0
81	732.5
82	519.0
83	319.5
84	120.0
85	90.5
86	61.0
87	52.5
88	44.0
89	30.5
90	17.0
91	13.0
92	9.0
93	4.5
94	0.0
95	2.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2642905.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.564318557024205
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.63414225728923	17.822670219380495
2	7.5227304034680085	3.545360312918628
3	3.5104533507037896	2.481643231066714
4	2.103527965217074	1.9827281226393625
5	1.3117707922000792	1.5455492410601335
6	0.9530021713617939	1.3474108050903049
7	0.6910900098641712	1.1399545600811432
8	0.5432472076927809	1.0241000205829263
9	0.43773180776321685	0.9283366584607072
>10	5.507182077340403	31.361478457638498
>50	1.5641306733466902	25.58515650409476
>100	0.21640134902013233	6.762477713311113
>500	0.0024588936068515445	0.4123163554087231
>1k	0.001803188645024466	0.9254906838333892
>5k	1.6392624045676962E-4	0.22871698898023698
>10k+	1.6392624045676962E-4	2.906610125452949
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	75246	2.84709439045293	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTT	5921	0.22403378100991142	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCT	4483	0.16962395545810385	No Hit
CGTTCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTC	3269	0.12368965210629969	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3206	0.12130591148754874	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.783715267858663E-5	0.0	0.0	0.03730743254108642	0.0
2	3.783715267858663E-5	0.0	0.0	0.10276570667504129	0.0
3	3.783715267858663E-5	0.0	0.0	0.24261182297509748	0.0
4	3.783715267858663E-5	0.0	0.0	0.3781066667171162	0.0
5	3.783715267858663E-5	0.0	0.0	0.6935928457511715	0.0
6	3.783715267858663E-5	0.0	0.0	1.4526061284836194	0.0
7	3.783715267858663E-5	0.0	0.0	1.9764615073186513	0.0
8	3.783715267858663E-5	0.0	0.0	2.717350793918056	0.0
9	3.783715267858663E-5	0.0	0.0	3.1743479239700254	0.0
10	3.783715267858663E-5	0.0	0.0	3.6150750783702024	0.0
11	3.783715267858663E-5	0.0	0.0	3.939150291062297	0.0
12	3.783715267858663E-5	0.0	0.0	4.169729899485604	0.0
13	3.783715267858663E-5	0.0	0.0	4.317105609168699	0.0
14	3.783715267858663E-5	0.0	0.0	4.403866200260698	0.0
15	3.783715267858663E-5	0.0	0.0	4.471746052166083	0.0
16	3.783715267858663E-5	0.0	0.0	4.572619901207195	0.0
17	3.783715267858663E-5	0.0	0.0	4.68170441237956	0.0
18	3.783715267858663E-5	0.0	0.0	4.816177652999256	0.0
19	3.783715267858663E-5	0.0	0.0	4.9034679642287555	0.0
20	3.783715267858663E-5	0.0	0.0	4.999574332032366	0.0
21	3.783715267858663E-5	0.0	0.0	5.1048751279368725	0.0
22	3.783715267858663E-5	0.0	0.0	5.222018952629777	0.0
23	3.783715267858663E-5	0.0	0.0	5.355470590127152	0.0
24	3.783715267858663E-5	0.0	0.0	5.469738791216483	0.0
25	3.783715267858663E-5	0.0	0.0	5.566223530546879	0.0
26	3.783715267858663E-5	0.0	0.0	5.661005598006739	0.0
27	3.783715267858663E-5	0.0	0.0	5.7594957064290995	0.0
28	3.783715267858663E-5	0.0	0.0	5.862715458936284	0.0
29	3.783715267858663E-5	0.0	0.0	5.979480912102402	0.0
30	3.783715267858663E-5	0.0	0.0	6.126553924564068	0.0
31	7.567430535717326E-5	0.0	0.0	6.245854466959653	0.0
32	7.567430535717326E-5	0.0	0.0	6.381122287785599	0.0
33	7.567430535717326E-5	0.0	0.0	6.507120006205293	0.0
34	7.567430535717326E-5	0.0	0.0	6.637393322877667	0.0
35	7.567430535717326E-5	0.0	0.0	6.788363562065228	0.0
36	7.567430535717326E-5	0.0	0.0	6.9230638256009955	0.0
37	7.567430535717326E-5	0.0	0.0	7.063666684954624	0.0
38	7.567430535717326E-5	0.0	0.0	7.216339596012721	0.0
39	7.567430535717326E-5	0.0	0.0	7.430876251700307	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	32235	0.0	43.708702	1
ATAACGC	80	0.0	42.1875	11
TCGATAG	65	0.0	41.53846	1
TACGGGA	1085	0.0	40.852535	4
TATAGCG	155	0.0	40.64516	1
CGGTTTT	1145	0.0	39.497818	1
CATACGA	1100	0.0	39.272728	18
GCGTAAG	155	0.0	39.193546	1
CGACGGT	150	0.0	39.000004	28
ATAGCGG	395	0.0	38.734177	2
ACGGGAT	1145	0.0	38.515285	5
GTTTTTT	37475	0.0	38.467644	2
CTATGCG	100	0.0	38.25	1
CACGACC	1195	0.0	38.22176	27
GCGAGAC	1190	0.0	38.193275	21
CGTAAGG	395	0.0	38.16456	2
CGTTCGA	125	0.0	37.800003	14
GGGCGAT	4335	0.0	37.629757	7
ACACGAC	1210	0.0	37.56198	26
TAACGCG	60	1.5643309E-10	37.500004	1
>>END_MODULE