FastQCFastQC Report
Thu 26 May 2016
SRR1547581_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547581_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2442140
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81570.3340103351978183No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGT302.1665091E-645.00000422
TAATACG207.0348475E-445.01
GTCGCGC207.0348475E-445.039
TACCCGA207.0348475E-445.028
CGTATCA351.2126839E-745.023
GCGTTAA351.2126839E-745.027
TATTACG750.045.01
CGCAATA351.2126839E-745.013
TTCGCGA207.0348475E-445.032
GTCGGTA207.0348475E-445.024
AATCGGC253.8920993E-545.035
TTTAGCG1550.043.5483861
CGTTTTT46700.041.964671
CGACGGT5450.041.6972528
CGCTAGC650.041.5384610
CCGGTAT650.041.5384637
CACGACG5550.040.94594626
TGCGATG1650.040.909091
CGTAAGG3200.040.781252
GCGTAAG2050.040.6097561