FastQCFastQC Report
Thu 26 May 2016
SRR1547580_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547580_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1401528
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94250.6724803214777014No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG34940.2492993361531129No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC26590.18972150395853668No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG21870.15604397486172233No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG17340.12372210901244926No Hit
CTGTCTCTTATACACATCTGACGCTCCTTCAGTCGTATGCCGTCTTCTGCT16940.12086808112288874No Hit
GCTGTCTCTTATACACATCTGACGCTCCTTCAGTCGTATGCCGTCTTCTGC16020.1143038169768995No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA14740.10517092773030577No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGG351.2121745E-745.0000043
GATATCG351.2121745E-745.00000410
GCCAACG351.2121745E-745.0000041
AATCGGC551.8189894E-1245.00000415
CGGACTC351.2121745E-745.00000414
TCTGCGA207.0338167E-445.018
AAGTACG207.0338167E-445.01
AACCGAT207.0338167E-445.037
AGCCGGT207.0338167E-445.018
ATCTCGT207.0338167E-445.019
TCGTTCG253.8912447E-545.038
ATCTCGC253.8912447E-545.039
GGTACGT207.0338167E-445.08
ACCGGCC207.0338167E-445.041
TCCGCTT253.8912447E-545.010
CTCGTTT253.8912447E-545.011
CGTATTA406.8175723E-945.030
CTCGTAG453.8562575E-1045.01
CTCCGGT253.8912447E-545.030
CGAAACG207.0338167E-445.030