##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547580_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1401528
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.348182126935743	31.0	31.0	33.0	30.0	34.0
2	31.75985709882357	31.0	31.0	34.0	30.0	34.0
3	31.900734769480167	31.0	31.0	34.0	30.0	34.0
4	35.63215290739821	37.0	35.0	37.0	33.0	37.0
5	35.53242603786724	37.0	35.0	37.0	33.0	37.0
6	35.58621946903665	37.0	35.0	37.0	33.0	37.0
7	35.956647316357575	37.0	35.0	37.0	35.0	37.0
8	35.90274543212836	37.0	35.0	37.0	35.0	37.0
9	37.524598866380124	39.0	37.0	39.0	35.0	39.0
10	37.01269328903882	39.0	37.0	39.0	33.0	39.0
11	36.80354013619421	39.0	37.0	39.0	32.0	39.0
12	36.511215616099	39.0	35.0	39.0	32.0	39.0
13	36.378820116330175	39.0	35.0	39.0	32.0	39.0
14	37.51224092561832	40.0	36.0	41.0	32.0	41.0
15	37.700683111575366	40.0	36.0	41.0	33.0	41.0
16	37.74136157108527	40.0	36.0	41.0	33.0	41.0
17	37.671056875067784	40.0	36.0	41.0	33.0	41.0
18	37.58081251319988	39.0	36.0	41.0	32.0	41.0
19	37.58768786638583	39.0	36.0	41.0	32.0	41.0
20	37.48902626276464	39.0	35.0	41.0	32.0	41.0
21	37.36399772248574	39.0	35.0	41.0	32.0	41.0
22	37.2870631196808	39.0	35.0	41.0	32.0	41.0
23	37.19521479413897	39.0	35.0	41.0	32.0	41.0
24	37.12463611144408	39.0	35.0	41.0	32.0	41.0
25	37.05996669349453	39.0	35.0	41.0	32.0	41.0
26	37.05653401145036	39.0	35.0	41.0	32.0	41.0
27	37.03407423897346	39.0	35.0	41.0	32.0	41.0
28	36.974722588489136	39.0	35.0	41.0	31.0	41.0
29	36.94483378141571	39.0	35.0	41.0	31.0	41.0
30	36.84541086585498	39.0	35.0	41.0	31.0	41.0
31	36.74265016467741	39.0	35.0	41.0	31.0	41.0
32	36.62115277040487	39.0	35.0	41.0	30.0	41.0
33	36.545170699408075	39.0	35.0	41.0	30.0	41.0
34	36.48785682483689	39.0	35.0	41.0	30.0	41.0
35	36.373961847355176	39.0	35.0	41.0	30.0	41.0
36	36.334608370293	39.0	35.0	41.0	30.0	41.0
37	36.293385504963155	39.0	35.0	41.0	30.0	41.0
38	36.1839363894264	38.0	35.0	41.0	30.0	41.0
39	36.144010679772364	38.0	35.0	41.0	30.0	41.0
40	36.07997128847943	38.0	35.0	41.0	30.0	41.0
41	36.03058090883664	38.0	35.0	41.0	29.0	41.0
42	36.020967115890656	38.0	35.0	40.0	29.0	41.0
43	35.97242509603804	38.0	35.0	40.0	29.0	41.0
44	35.870624775245304	38.0	35.0	40.0	29.0	41.0
45	35.80170713678214	38.0	35.0	40.0	29.0	41.0
46	35.771546483552235	38.0	35.0	40.0	29.0	41.0
47	35.708055779121075	38.0	35.0	40.0	28.0	41.0
48	35.62235859718821	38.0	35.0	40.0	28.0	41.0
49	35.56407221261366	38.0	34.0	40.0	28.0	41.0
50	35.479003630323476	38.0	34.0	40.0	28.0	41.0
51	35.057794778270576	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	3.0
12	4.0
13	5.0
14	11.0
15	20.0
16	66.0
17	167.0
18	318.0
19	677.0
20	1293.0
21	2107.0
22	3383.0
23	5293.0
24	7742.0
25	11280.0
26	15136.0
27	18078.0
28	21462.0
29	25327.0
30	31371.0
31	38659.0
32	47805.0
33	61823.0
34	98636.0
35	136525.0
36	112515.0
37	136379.0
38	206427.0
39	418821.0
40	193.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.924085712165578	26.087170573830846	29.381075511869902	12.60766820213367
2	29.872039659571552	26.78712091374557	30.442203081208508	12.898636345474369
3	29.754810464007853	24.963325741619148	31.79636796410775	13.485495830265254
4	27.45974393661775	27.327388393239378	31.573967840813737	13.638899829329132
5	24.412569709631203	31.786735619980476	29.745962977550217	14.054731692838102
6	21.855931526162877	41.622714637167434	26.18206700115874	10.339286835510956
7	85.61448647476183	5.0353614055516545	7.165465120925162	2.184686998761352
8	85.83453202504695	4.600621607274346	6.727086437088663	2.8377599305900416
9	80.00767733502292	7.751611098743657	8.671606988943497	3.569104577289929
10	38.55677517680703	32.14677123824854	17.99007939905589	11.306374185888544
11	30.808446210136363	26.375998196254375	24.861294244567357	17.954261349041904
12	28.327083012255194	23.34601948730243	29.58449634969833	18.742401150744044
13	24.222919556369902	29.56701542887477	29.00077629558596	17.209288719169365
14	17.97680816936943	33.27461170950563	29.59384329103664	19.154736830088304
15	17.767322522275688	31.361414113738718	33.924759262747514	16.946504101238077
16	19.902492137153164	30.78918152188183	33.43779075409125	15.870535586873755
17	20.74464441666524	31.068519501572567	26.711489174672217	21.475346907089975
18	20.74257524644531	31.99122671826749	30.126476245925875	17.139721789361325
19	23.618365098663745	32.905015097807535	25.829808608889728	17.646811194638996
20	23.723179272907853	32.19008111147262	27.49113824340291	16.59560137221661
21	22.628802278655865	31.820841253260728	28.927713181613214	16.622643286470197
22	21.904307298890924	27.449612137609808	28.702459030429644	21.94362153306962
23	19.8434851105365	30.3010000513725	29.029459275876047	20.826055562214954
24	18.64529285180175	29.671686901724403	32.18187578129014	19.501144465183714
25	19.316417510031908	32.06828547128563	28.7693146337426	19.845982384939866
26	18.349758263837753	35.83124989297396	27.091645689561677	18.727346153626616
27	18.963088857304314	34.4048067537716	28.176104936897445	18.455999452026646
28	16.714400283119566	32.75218190432157	31.797652276658045	18.735765535900818
29	18.41447334623354	29.8428572244008	31.53344064478198	20.209228784583683
30	19.665322419530685	31.231199091277517	28.919507851430726	20.18397063776107
31	22.12891929379934	30.15844135828895	27.241624855158086	20.47101449275362
32	20.937648052696773	31.47050932981717	28.52843467986369	19.06340793762237
33	21.243100387576987	30.850115017323947	27.324962469533254	20.58182212556581
34	18.608547242723656	30.621079279186713	29.187643771654937	21.58272970643469
35	19.44634712970415	29.53248169141109	30.141673944437784	20.879497234446976
36	21.29647070911177	30.86174518097391	28.28534285437037	19.556441255543948
37	18.875898305278238	33.17022563944495	28.187663749850163	19.76621230542665
38	18.968083406111045	33.49829614534993	27.265384637338673	20.26823581120035
39	20.543364099753983	31.1053364613479	28.251308571787366	20.099990867110755
40	20.663946778087915	30.40374505539668	28.272071624683914	20.660236541831488
41	17.574247535546917	30.43078696965027	28.346633103298686	23.64833239150413
42	20.431628907877688	29.370943712861962	27.637335822045657	22.560091557214697
43	19.883869605173782	28.597644856185532	28.461436375156257	23.057049163484425
44	18.848142884052262	31.028277708329767	28.975018693882674	21.148560713735296
45	19.164083771426615	32.36303520157999	27.603943695737794	20.8689373312556
46	20.91802661095604	31.826620659737088	26.986403411134134	20.268949318172737
47	19.62279740397623	30.04014190226667	27.939006569972204	22.398054123784895
48	18.379440153889185	30.26618091111986	29.806539719506137	21.547839215484814
49	19.52904258780417	28.427544793967723	30.86153112888219	21.181881489345912
50	18.774723016593317	29.913137661181228	29.314362609951424	21.997776712274035
51	18.430884006598514	29.331772180077742	28.76867247746745	23.468671335856296
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1710.0
1	2410.0
2	3110.0
3	8415.0
4	13720.0
5	9390.0
6	5060.0
7	5250.0
8	5440.0
9	6544.5
10	7649.0
11	8776.0
12	9903.0
13	9868.0
14	9833.0
15	9108.5
16	8384.0
17	7744.0
18	7104.0
19	6894.0
20	6684.0
21	6957.0
22	7230.0
23	8117.0
24	9004.0
25	11757.5
26	16481.0
27	18451.0
28	24538.5
29	30626.0
30	35349.0
31	40072.0
32	44655.0
33	49238.0
34	51207.0
35	53176.0
36	58176.0
37	63176.0
38	67610.5
39	72045.0
40	82690.5
41	93336.0
42	98906.0
43	104476.0
44	112293.0
45	120110.0
46	123718.0
47	127326.0
48	130483.0
49	133640.0
50	121215.0
51	108790.0
52	91055.5
53	73321.0
54	64694.5
55	56068.0
56	51236.5
57	46405.0
58	41932.0
59	37459.0
60	33472.0
61	29485.0
62	23020.5
63	16556.0
64	12751.5
65	8947.0
66	6662.5
67	4378.0
68	3364.0
69	2350.0
70	1802.0
71	1254.0
72	971.5
73	689.0
74	497.0
75	283.0
76	261.0
77	191.0
78	121.0
79	103.0
80	85.0
81	51.5
82	18.0
83	17.0
84	16.0
85	9.0
86	2.0
87	2.0
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1401528.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.08335418300235
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.29158008643677	15.452323415942285
2	11.158715900772842	4.705263191269879
3	4.439155340146529	2.807768529290266
4	2.3679888243911713	1.9970058834412168
5	1.3725245058823474	1.4468710141183911
6	0.9284804874783688	1.1745289781707877
7	0.5916860393257833	0.8732308432570342
8	0.45040692085826073	0.7596870911144176
9	0.3652338464140381	0.6930319089210704
>10	3.0422377352576344	14.27822670966264
>50	0.907972252920728	13.866724449316642
>100	1.073400505656278	38.891612992134455
>500	0.006165031621509925	0.8719461158890912
>1k	0.004110021081006617	1.5011915364984114
>5k	3.4250175675055143E-4	0.6805873409734728
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9425	0.6724803214777014	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3494	0.2492993361531129	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	2659	0.18972150395853668	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2187	0.15604397486172233	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1734	0.12372210901244926	No Hit
CTGTCTCTTATACACATCTGACGCTCCTTCAGTCGTATGCCGTCTTCTGCT	1694	0.12086808112288874	No Hit
GCTGTCTCTTATACACATCTGACGCTCCTTCAGTCGTATGCCGTCTTCTGC	1602	0.1143038169768995	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1474	0.10517092773030577	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1308571787363506	0.0
2	0.0	0.0	0.0	0.46435033763149935	0.0
3	0.0	0.0	0.0	0.6801148460822759	0.0
4	0.0	0.0	0.0	1.0035475566667238	0.0
5	0.0	0.0	0.0	1.728185237826144	0.0
6	0.0	0.0	0.0	2.3577837902631984	0.0
7	0.0	0.0	0.0	2.7960911234024577	0.0
8	0.0	0.0	0.0	3.7409884069387127	0.0
9	0.0	0.0	0.0	4.014618330850329	0.0
10	0.0	0.0	0.0	4.588277936651997	0.0
11	0.0	0.0	0.0	5.558290665616385	0.0
12	0.0	0.0	0.0	6.198092367758618	0.0
13	0.0	0.0	0.0	6.460234829414753	0.0
14	0.0	0.0	0.0	6.562123625072064	0.0
15	0.0	0.0	0.0	6.722805395254323	0.0
16	0.0	0.0	0.0	7.124295768618251	0.0
17	0.0	0.0	0.0	7.6101940168159325	0.0
18	0.0	0.0	0.0	8.124989297395414	0.0
19	0.0	0.0	0.0	8.47096882830739	0.0
20	0.0	0.0	0.0	8.795186396561467	0.0
21	0.0	0.0	0.0	9.23320832691177	0.0
22	0.0	0.0	0.0	9.711543401202116	0.0
23	0.0	0.0	0.0	10.240180717045966	0.0
24	0.0	0.0	0.0	10.597148255332751	0.0
25	0.0	0.0	0.0	10.933281390025744	0.0
26	0.0	0.0	0.0	11.232526214246166	0.0
27	0.0	0.0	0.0	11.53733639285123	0.0
28	0.0	0.0	0.0	11.886526705138962	0.0
29	0.0	0.0	0.0	12.241139670416858	0.0
30	0.0	0.0	0.0	12.628645307121941	0.0
31	0.0	0.0	0.0	13.00687535318595	0.0
32	0.0	0.0	0.0	13.361488318463849	0.0
33	0.0	0.0	0.0	13.716957492108612	0.0
34	0.0	0.0	0.0	14.0693585857721	0.0
35	0.0	0.0	0.0	14.447945385322305	0.0
36	0.0	0.0	0.0	14.821894389551975	0.0
37	0.0	0.0	0.0	15.204904932330999	0.0
38	0.0	0.0	0.0	15.599616989457221	0.0
39	0.0	0.0	0.0	16.01708991900269	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACCGG	35	1.2121745E-7	45.000004	3
GATATCG	35	1.2121745E-7	45.000004	10
GCCAACG	35	1.2121745E-7	45.000004	1
AATCGGC	55	1.8189894E-12	45.000004	15
CGGACTC	35	1.2121745E-7	45.000004	14
TCTGCGA	20	7.0338167E-4	45.0	18
AAGTACG	20	7.0338167E-4	45.0	1
AACCGAT	20	7.0338167E-4	45.0	37
AGCCGGT	20	7.0338167E-4	45.0	18
ATCTCGT	20	7.0338167E-4	45.0	19
TCGTTCG	25	3.8912447E-5	45.0	38
ATCTCGC	25	3.8912447E-5	45.0	39
GGTACGT	20	7.0338167E-4	45.0	8
ACCGGCC	20	7.0338167E-4	45.0	41
TCCGCTT	25	3.8912447E-5	45.0	10
CTCGTTT	25	3.8912447E-5	45.0	11
CGTATTA	40	6.8175723E-9	45.0	30
CTCGTAG	45	3.8562575E-10	45.0	1
CTCCGGT	25	3.8912447E-5	45.0	30
CGAAACG	20	7.0338167E-4	45.0	30
>>END_MODULE