FastQCFastQC Report
Thu 26 May 2016
SRR1547579_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547579_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1577078
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72200.45780868162513205No Hit
CTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGCT32400.20544323108939444Illumina PCR Primer Index 10 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC30380.1926347333486359Illumina PCR Primer Index 10 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC27140.17209041023969646Illumina PCR Primer Index 10 (95% over 23bp)
GCCCTGCTAGAGGACTTGCGTCTGTATCTATGCTCTGCCCCATGCAGCTCC21250.13474285989659357No Hit
TACGTAGGGGTTTTCTTGTAACTACTTTGCAGATTCTAAAAGTAGTTATTG16250.1030386575679833No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16080.10196071468881057No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGGC302.166018E-645.00000425
CATGCGA302.166018E-645.00000436
TAGCGTC302.166018E-645.00000443
TGGGTCG302.166018E-645.0000046
CGCATAC302.166018E-645.00000443
GTCTACG302.166018E-645.0000041
TAACGTT302.166018E-645.00000430
GTTACGC302.166018E-645.00000418
TGCGACC302.166018E-645.00000438
ACGGATC302.166018E-645.00000436
GTCGTAG950.045.0000041
CGGGACG1200.045.0000046
CCGGTCA302.166018E-645.00000431
ACCGTCA302.166018E-645.00000431
ATCGAAA302.166018E-645.00000439
ACGCGGT302.166018E-645.00000411
TCACGCG207.0340873E-445.01
CCTTCGC253.8914688E-545.023
TCGCCCA351.21232E-745.019
CTTCGCA253.8914688E-545.024