##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547579_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1577078
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27820183909737	31.0	31.0	33.0	30.0	34.0
2	31.678467393496074	31.0	31.0	34.0	30.0	34.0
3	31.818346334169902	31.0	31.0	34.0	30.0	34.0
4	35.56851404940022	37.0	35.0	37.0	33.0	37.0
5	35.4615415344073	37.0	35.0	37.0	33.0	37.0
6	35.5052077322745	37.0	35.0	37.0	33.0	37.0
7	35.90138027415258	37.0	35.0	37.0	35.0	37.0
8	35.8262920413575	37.0	35.0	37.0	35.0	37.0
9	37.444983697699165	39.0	37.0	39.0	35.0	39.0
10	36.92393654594129	39.0	37.0	39.0	32.0	39.0
11	36.74343881532809	39.0	35.0	39.0	32.0	39.0
12	36.45042857740708	39.0	35.0	39.0	32.0	39.0
13	36.32410888998515	39.0	35.0	39.0	32.0	39.0
14	37.41375886290976	39.0	36.0	41.0	32.0	41.0
15	37.565966299701095	39.0	36.0	41.0	32.0	41.0
16	37.59895642447615	39.0	36.0	41.0	32.0	41.0
17	37.539544651564476	39.0	36.0	41.0	32.0	41.0
18	37.46499602429303	39.0	36.0	41.0	32.0	41.0
19	37.46602070411229	39.0	36.0	41.0	32.0	41.0
20	37.37217753338769	39.0	35.0	41.0	32.0	41.0
21	37.25122726967214	39.0	35.0	41.0	32.0	41.0
22	37.150027455839215	39.0	35.0	41.0	32.0	41.0
23	37.05140455957156	39.0	35.0	41.0	31.0	41.0
24	36.981687018650945	39.0	35.0	41.0	31.0	41.0
25	36.891128403287595	38.0	35.0	41.0	31.0	41.0
26	36.90447587246794	39.0	35.0	41.0	31.0	41.0
27	36.86044190585374	39.0	35.0	41.0	31.0	41.0
28	36.812808878191184	39.0	35.0	41.0	31.0	41.0
29	36.78434167492033	39.0	35.0	41.0	31.0	41.0
30	36.67905392123915	38.0	35.0	41.0	31.0	41.0
31	36.58070304702748	38.0	35.0	40.0	30.0	41.0
32	36.43763910218772	38.0	35.0	41.0	30.0	41.0
33	36.34910067859675	38.0	35.0	41.0	30.0	41.0
34	36.31891827797991	38.0	35.0	41.0	30.0	41.0
35	36.18852713689494	38.0	35.0	41.0	30.0	41.0
36	36.15710383379896	38.0	35.0	41.0	30.0	41.0
37	36.11217073600672	38.0	35.0	41.0	30.0	41.0
38	35.97452820976515	38.0	35.0	40.0	29.0	41.0
39	35.9065556681407	38.0	35.0	40.0	29.0	41.0
40	35.862233193285306	38.0	35.0	40.0	29.0	41.0
41	35.85111009094033	38.0	35.0	40.0	29.0	41.0
42	35.81523298150123	38.0	35.0	40.0	29.0	41.0
43	35.75579077255532	38.0	35.0	40.0	28.0	41.0
44	35.62483022399653	38.0	35.0	40.0	28.0	41.0
45	35.54738129629606	38.0	34.0	40.0	27.0	41.0
46	35.4599430085259	38.0	34.0	40.0	27.0	41.0
47	35.38992427768316	38.0	34.0	40.0	27.0	41.0
48	35.305852342116246	38.0	34.0	40.0	27.0	41.0
49	35.24670561633604	38.0	34.0	40.0	27.0	41.0
50	35.138849822266245	38.0	34.0	40.0	26.0	41.0
51	34.6889240735081	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	4.0
12	6.0
13	5.0
14	19.0
15	37.0
16	73.0
17	173.0
18	428.0
19	797.0
20	1472.0
21	2537.0
22	4116.0
23	6413.0
24	9720.0
25	13892.0
26	18508.0
27	21689.0
28	25499.0
29	30302.0
30	36919.0
31	46196.0
32	57476.0
33	73837.0
34	112334.0
35	161553.0
36	126502.0
37	156174.0
38	233332.0
39	436913.0
40	149.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.969530993394116	25.604377208990297	29.449779909427438	10.976311888188157
2	31.517971844132	26.412707551560544	28.93997633598338	13.129344268324077
3	29.387639672863358	24.45795325278775	31.390267317152354	14.764139757196537
4	27.625646924248514	27.195674532267905	30.519669921208713	14.659008622274866
5	24.417752324235074	31.813835460262585	28.929070090382343	14.83934212512
6	22.8197337100638	40.275370019745374	26.352976834373443	10.551919435817378
7	84.4730571347771	5.028983981768816	7.691185851302218	2.806773032151866
8	83.29981142340455	5.036846623946311	7.676348284612429	3.9869936680367113
9	77.56826231803373	7.530065095068221	10.190364712461907	4.711307874436141
10	39.13300420144089	26.909766035668497	20.01258022748399	13.94464953540662
11	30.169782344310175	28.343113022944966	23.205447035593675	18.281657597151188
12	28.159038424225052	23.734716989267493	28.360233292202413	19.74601129430504
13	23.25566649208219	30.108846867434586	29.330889150695143	17.304597489788076
14	18.174053534447886	33.21604892085236	29.393409837687166	19.21648770701259
15	18.478096834779258	30.734751229806008	34.28251487878215	16.504637056632582
16	19.195309299857076	29.310915503228124	33.94112402810768	17.552651168807124
17	19.878851902061914	30.890418863239482	26.38677351405574	22.843955720642857
18	20.911521180309407	29.549077471120643	31.305934138958253	18.2334672096117
19	22.765265890463247	32.674667961889014	26.37751588697579	18.182550260671952
20	24.344452208451326	30.32468907688776	26.991436060866995	18.339422653793918
21	22.24766308324636	31.822078552868028	28.494025026029153	17.436233337856464
22	21.56386684742289	26.98192480016841	28.094425259879348	23.359783092529348
23	19.19790904444802	31.944710407475092	27.644986487668966	21.21239406040792
24	20.225822692346227	29.438620030207762	31.379487888360625	18.956069389085386
25	19.19657746795022	31.168274492447424	28.439049939191342	21.196098100411014
26	18.31348861628911	34.90664380582317	27.243547877784103	19.53631970010361
27	20.420169452620605	33.97187710436643	27.20391762487334	18.404035818139622
28	18.091939650416784	31.047671706789394	31.53502870498479	19.325359937809036
29	18.39363683977584	28.252819454713084	31.787584380734497	21.565959324776582
30	19.61957493541854	30.50616393101673	29.650087059739594	20.22417407382514
31	22.13308409603076	30.530702983619072	25.98666648066868	21.34954643968149
32	22.422923913718915	28.97345597364239	29.13210380209476	19.47151631054393
33	22.077157883123093	28.62153932779482	27.349376505157007	21.951926283925083
34	19.56180987877581	29.06685655370248	28.73618172341508	22.635151844106634
35	18.452923698130338	28.069759390467684	31.221347327145523	22.255969584256455
36	22.00417480936263	28.657238259616836	27.975154050719116	21.36343288030142
37	19.781329775699106	30.124128292956975	29.58243029197034	20.512111639373575
38	18.594261032111284	31.81567430399765	28.713354697738474	20.876709966152593
39	19.529217958782	30.507368690705217	28.16804241768638	21.795370932826405
40	20.89871268256865	28.86902233117195	28.57366598227862	21.65859900398078
41	17.651251238049102	28.218832549816813	28.161574760411344	25.968341451722743
42	20.034265901876765	27.460721663735082	28.278880309027198	24.22613212536095
43	20.170403746675813	27.889108845599264	29.268241646893813	22.672245760831107
44	19.552362026481887	29.156325812673817	28.517612952561638	22.77369920828266
45	20.255878276153748	30.374464674543685	27.071140425521122	22.29851662378145
46	21.764110589330393	29.622377586904392	27.58011968970463	21.033392134060584
47	19.49928919178379	28.4764608979391	29.133181744973935	22.891068165303174
48	19.190173219079842	26.50509359714611	30.724479068251537	23.580254115522504
49	20.64596678160497	25.60177746439935	31.312845655065885	22.439410098929795
50	18.61334696191311	27.442206409575178	30.715158032766926	23.229288595744787
51	17.475483140339286	28.146293334888956	28.743980957187915	25.634242567583847
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1913.0
1	2285.0
2	2657.0
3	6744.0
4	10831.0
5	7781.5
6	4732.0
7	5246.0
8	5760.0
9	6719.0
10	7678.0
11	8666.0
12	9654.0
13	9172.0
14	8690.0
15	8379.0
16	8068.0
17	7770.5
18	7473.0
19	7179.5
20	6886.0
21	6894.5
22	6903.0
23	8136.0
24	9369.0
25	10862.0
26	13354.0
27	14353.0
28	18437.5
29	22522.0
30	27566.0
31	32610.0
32	36989.5
33	41369.0
34	47106.0
35	52843.0
36	58755.5
37	64668.0
38	70414.0
39	76160.0
40	86312.0
41	96464.0
42	109965.0
43	123466.0
44	133527.5
45	143589.0
46	156721.0
47	169853.0
48	173977.0
49	178101.0
50	160618.0
51	143135.0
52	120351.5
53	97568.0
54	83121.0
55	68674.0
56	59923.5
57	51173.0
58	43401.0
59	35629.0
60	30865.0
61	26101.0
62	21574.0
63	17047.0
64	13114.5
65	9182.0
66	6993.0
67	4804.0
68	3545.5
69	2287.0
70	1590.0
71	893.0
72	905.0
73	917.0
74	695.0
75	293.5
76	114.0
77	89.5
78	65.0
79	37.5
80	10.0
81	13.0
82	16.0
83	13.5
84	11.0
85	7.0
86	3.0
87	3.0
88	3.0
89	2.0
90	1.0
91	1.5
92	2.0
93	1.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1577078.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.419855530671857
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.38857263470655	16.953299131763735
2	11.651605492068201	5.457578346492401
3	4.9046080657044255	3.445956370000968
4	2.5781719726482475	2.4152166053059694
5	1.5475817660949875	1.8122070691923307
6	0.9493185069890149	1.33397413717655
7	0.6818714355555003	1.117853135784115
8	0.5202605878450428	0.9747542244506654
9	0.37709503489682794	0.7948360114755635
>10	2.798707480142589	14.042259420698736
>50	0.7288757066309983	12.334710153628912
>100	0.8643025155123378	36.60389796956802
>500	0.005472000730497931	0.8701620380238284
>1k	0.0032832004382987584	1.3806613721133603
>5k	2.7360003652489653E-4	0.4626340143248926
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7220	0.45780868162513205	No Hit
CTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGCT	3240	0.20544323108939444	Illumina PCR Primer Index 10 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC	3038	0.1926347333486359	Illumina PCR Primer Index 10 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC	2714	0.17209041023969646	Illumina PCR Primer Index 10 (95% over 23bp)
GCCCTGCTAGAGGACTTGCGTCTGTATCTATGCTCTGCCCCATGCAGCTCC	2125	0.13474285989659357	No Hit
TACGTAGGGGTTTTCTTGTAACTACTTTGCAGATTCTAAAAGTAGTTATTG	1625	0.1030386575679833	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1608	0.10196071468881057	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.26816809314441E-4	0.0	0.0	0.22269031715615842	0.0
2	1.26816809314441E-4	0.0	0.0	0.8065549072398448	0.0
3	1.26816809314441E-4	0.0	0.0	1.1533354723101839	0.0
4	1.26816809314441E-4	0.0	0.0	1.7073347038003193	0.0
5	1.26816809314441E-4	0.0	0.0	2.963518608464515	0.0
6	1.26816809314441E-4	0.0	0.0	4.013054522350829	0.0
7	1.26816809314441E-4	0.0	0.0	4.673579873665094	0.0
8	1.26816809314441E-4	0.0	0.0	6.090884534563287	0.0
9	1.26816809314441E-4	0.0	0.0	6.464803896826917	0.0
10	1.26816809314441E-4	0.0	0.0	7.307057735888777	0.0
11	1.26816809314441E-4	0.0	0.0	8.591268155411463	0.0
12	1.26816809314441E-4	0.0	0.0	9.48970184099962	0.0
13	1.26816809314441E-4	0.0	0.0	9.867362299138026	0.0
14	1.26816809314441E-4	0.0	0.0	10.031907109223514	0.0
15	1.26816809314441E-4	0.0	0.0	10.290867033843602	0.0
16	1.26816809314441E-4	0.0	0.0	10.92190747699226	0.0
17	1.26816809314441E-4	0.0	0.0	11.622760573668518	0.0
18	1.26816809314441E-4	0.0	0.0	12.337373294155393	0.0
19	1.26816809314441E-4	0.0	0.0	12.80488346169308	0.0
20	1.26816809314441E-4	0.0	0.0	13.270491377091052	0.0
21	1.26816809314441E-4	0.0	0.0	13.874266206237104	0.0
22	1.26816809314441E-4	0.0	0.0	14.526992323778533	0.0
23	1.26816809314441E-4	0.0	0.0	15.258725313522858	0.0
24	1.26816809314441E-4	0.0	0.0	15.754705854751636	0.0
25	1.26816809314441E-4	0.0	0.0	16.243077387421547	0.0
26	1.26816809314441E-4	0.0	0.0	16.680088112319112	0.0
27	1.26816809314441E-4	0.0	0.0	17.105051240331804	0.0
28	1.26816809314441E-4	0.0	0.0	17.577443855028097	0.0
29	1.26816809314441E-4	0.0	0.0	18.057191844664626	0.0
30	1.26816809314441E-4	0.0	0.0	18.57447760985823	0.0
31	1.26816809314441E-4	0.0	0.0	19.07800375124122	0.0
32	1.26816809314441E-4	0.0	0.0	19.521545541818476	0.0
33	1.26816809314441E-4	0.0	0.0	19.9964110842964	0.0
34	1.26816809314441E-4	0.0	0.0	20.450985937284017	0.0
35	1.26816809314441E-4	0.0	0.0	20.93314344629752	0.0
36	1.26816809314441E-4	0.0	0.0	21.3944395901788	0.0
37	1.26816809314441E-4	0.0	0.0	21.872665778103556	0.0
38	1.26816809314441E-4	0.0	0.0	22.35051151560037	0.0
39	1.26816809314441E-4	0.0	0.0	22.84002439955411	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGGC	30	2.166018E-6	45.000004	25
CATGCGA	30	2.166018E-6	45.000004	36
TAGCGTC	30	2.166018E-6	45.000004	43
TGGGTCG	30	2.166018E-6	45.000004	6
CGCATAC	30	2.166018E-6	45.000004	43
GTCTACG	30	2.166018E-6	45.000004	1
TAACGTT	30	2.166018E-6	45.000004	30
GTTACGC	30	2.166018E-6	45.000004	18
TGCGACC	30	2.166018E-6	45.000004	38
ACGGATC	30	2.166018E-6	45.000004	36
GTCGTAG	95	0.0	45.000004	1
CGGGACG	120	0.0	45.000004	6
CCGGTCA	30	2.166018E-6	45.000004	31
ACCGTCA	30	2.166018E-6	45.000004	31
ATCGAAA	30	2.166018E-6	45.000004	39
ACGCGGT	30	2.166018E-6	45.000004	11
TCACGCG	20	7.0340873E-4	45.0	1
CCTTCGC	25	3.8914688E-5	45.0	23
TCGCCCA	35	1.21232E-7	45.0	19
CTTCGCA	25	3.8914688E-5	45.0	24
>>END_MODULE