FastQCFastQC Report
Thu 26 May 2016
SRR1547578_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547578_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1846594
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55650.3013656494064207No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT20330.11009458494937166No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC19830.10738689717393211No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTG207.034399E-445.00000426
CGAACGC207.034399E-445.00000442
CGTATTC207.034399E-445.00000434
GGTAATC207.034399E-445.0000049
CGTATCC207.034399E-445.00000420
GACACGC302.16622E-645.00000413
CGCCTAA207.034399E-445.00000420
CCGTATG207.034399E-445.0000045
CCCGTAC207.034399E-445.00000426
CTACGAT207.034399E-445.00000412
TCGTCGA207.034399E-445.00000440
CGTCGTT207.034399E-445.00000412
ACCGTCC207.034399E-445.00000435
TACGTCG207.034399E-445.00000410
GTACGAT302.16622E-645.00000411
GACTACG302.16622E-645.00000430
AACCGTC351.2124656E-745.034
CCCTCGA351.2124656E-745.020
AACCCGC351.2124656E-745.021
TTTCGCA453.8562575E-1045.040