##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1547578_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 1846594 Sequences flagged as poor quality 0 Sequence length 51 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.39510200942925 31.0 31.0 33.0 30.0 34.0 2 31.823867076357878 31.0 31.0 34.0 30.0 34.0 3 31.99003625052394 33.0 31.0 34.0 30.0 34.0 4 35.70232871979439 37.0 35.0 37.0 33.0 37.0 5 35.594520506402596 37.0 35.0 37.0 33.0 37.0 6 35.629911610240256 37.0 35.0 37.0 33.0 37.0 7 35.96185355308205 37.0 35.0 37.0 35.0 37.0 8 35.90580928996845 37.0 35.0 37.0 35.0 37.0 9 37.543389071988756 39.0 37.0 39.0 35.0 39.0 10 37.12880308286499 39.0 37.0 39.0 33.0 39.0 11 36.90921881041528 39.0 37.0 39.0 33.0 39.0 12 36.465139602966325 39.0 35.0 39.0 32.0 39.0 13 36.274473977495866 39.0 35.0 39.0 32.0 39.0 14 37.22845790682738 40.0 35.0 41.0 31.0 41.0 15 37.46970963839372 40.0 35.0 41.0 32.0 41.0 16 37.56498017431011 40.0 35.0 41.0 32.0 41.0 17 37.533864509469865 40.0 35.0 41.0 32.0 41.0 18 37.459589926101785 39.0 36.0 41.0 32.0 41.0 19 37.4846409118626 39.0 36.0 41.0 32.0 41.0 20 37.37606263206747 39.0 35.0 41.0 32.0 41.0 21 37.24696711892273 39.0 35.0 41.0 32.0 41.0 22 37.18862998580089 39.0 35.0 41.0 32.0 41.0 23 37.1235458362802 39.0 35.0 41.0 32.0 41.0 24 37.05012904839938 39.0 35.0 41.0 32.0 41.0 25 36.976733922020756 39.0 35.0 41.0 31.0 41.0 26 37.016390717179846 39.0 35.0 41.0 32.0 41.0 27 36.975919991075465 39.0 35.0 41.0 31.0 41.0 28 36.95646742055915 39.0 35.0 41.0 31.0 41.0 29 36.89978793389343 39.0 35.0 41.0 31.0 41.0 30 36.83049928679504 39.0 35.0 41.0 31.0 41.0 31 36.68572192913006 39.0 35.0 41.0 31.0 41.0 32 36.59498135486198 39.0 35.0 41.0 30.0 41.0 33 36.52830995876733 39.0 35.0 41.0 30.0 41.0 34 36.45083759613645 39.0 35.0 41.0 30.0 41.0 35 36.364521383693436 39.0 35.0 41.0 30.0 41.0 36 36.31147344787214 39.0 35.0 41.0 30.0 41.0 37 36.2764305526824 39.0 35.0 41.0 30.0 41.0 38 36.163954827103304 39.0 35.0 41.0 30.0 41.0 39 36.10761975832262 39.0 35.0 41.0 29.0 41.0 40 36.00917797848363 38.0 35.0 41.0 29.0 41.0 41 35.94497057826463 38.0 35.0 41.0 29.0 41.0 42 35.93292786611459 38.0 35.0 41.0 29.0 41.0 43 35.929408413544074 38.0 35.0 40.0 29.0 41.0 44 35.830634129646256 38.0 35.0 40.0 29.0 41.0 45 35.75764786412173 38.0 35.0 40.0 28.0 41.0 46 35.7436106691563 38.0 35.0 40.0 29.0 41.0 47 35.690389441317365 38.0 34.0 40.0 28.0 41.0 48 35.60568159541296 38.0 34.0 40.0 28.0 41.0 49 35.51271800948124 38.0 34.0 40.0 28.0 41.0 50 35.425888419436 38.0 34.0 40.0 28.0 41.0 51 34.97914484721601 37.0 34.0 40.0 27.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 10.0 11 7.0 12 6.0 13 13.0 14 28.0 15 47.0 16 153.0 17 315.0 18 676.0 19 1249.0 20 2140.0 21 3500.0 22 5219.0 23 7786.0 24 11188.0 25 15834.0 26 20605.0 27 25071.0 28 28645.0 29 33912.0 30 40783.0 31 50164.0 32 63156.0 33 81437.0 34 134688.0 35 179106.0 36 127771.0 37 174044.0 38 279700.0 39 559174.0 40 165.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.26216049656828 24.195681346305683 29.04829106993741 12.49386708718863 2 31.600720028333246 25.02537103445587 28.957096145660604 14.416812791550282 3 30.733176865082417 24.981885568782307 30.014556529480764 14.270381036654511 4 28.29636617469785 27.48037738669139 28.819653914179295 15.403602524431466 5 24.71144171377141 31.33130509467701 28.369906974678788 15.587346216872794 6 23.24067986790816 41.06230172956265 25.346069574578927 10.350948827950269 7 87.55552113783538 4.5060256883754635 5.779722017942222 2.158731155846927 8 87.32623413701117 3.8986371665888657 6.265372897345058 2.5097557990549086 9 81.50421803601658 6.697574020060718 8.336483276778761 3.4617246671439417 10 38.97851937133988 38.2190671040846 13.612737829755755 9.18967569481976 11 32.654010572979224 23.960870662419566 27.08852081182978 16.296597952771428 12 31.906038901891808 22.5922427994459 28.647011741617266 16.854706557045024 13 26.819755723239652 27.842449396023166 28.106665569150557 17.231129311586628 14 18.515385623477602 32.28933918338303 29.54136101384495 19.65391417929442 15 17.464207075296464 30.070659820187874 36.17070130196459 16.294431802551077 16 23.66508284983055 27.522238239699686 33.38286596837204 15.42981294209772 17 24.068799097148588 27.00035849786147 26.49775749298438 22.43308491200556 18 22.82775748215363 28.88658795598816 29.610948589673747 18.674705972184466 19 25.320996385778354 30.037571875572 26.58575734568617 18.05567439296348 20 26.110179064807966 30.65367915199551 26.48627689681652 16.749864886380006 21 26.85473904929833 26.159783904854017 31.094274106815035 15.89120293903262 22 24.802149254248633 23.287414558912246 30.792800149897598 21.117636036941526 23 20.34139610547852 27.92102649526642 31.75045516231505 19.98712223694001 24 20.39370863330001 27.986985769476124 32.983265406472675 18.63604019075119 25 21.880824913326915 29.6137645849602 29.110730350039045 19.39468015167384 26 19.438977923680028 32.812789384131 25.968566994152475 21.779665698036492 27 19.13977842449396 32.21059962287325 30.616962905760552 18.03265904687224 28 17.41687669298178 30.10607637629062 32.14967664792586 20.327370282801745 29 19.208228771457073 29.099682983915255 31.825566421205743 19.86652182342193 30 21.866420014361577 27.937976620740674 30.58842387660742 19.60717948829033 31 23.382833476118737 27.950594445774218 28.61376133573487 20.052810742372174 32 24.602105281399158 28.28672680621728 27.773674126527 19.337493785856555 33 24.078384311873645 27.63715250888934 27.88322717392128 20.40123600531573 34 20.419377513411177 28.287322497527885 30.340887060176737 20.952412928884208 35 20.018910491423668 28.275679440093494 29.81732855191775 21.88808151656509 36 24.03901453161875 28.22872813406737 27.54904434867654 20.18321298563734 37 20.385639723729202 30.049973085583513 29.09610883605167 20.468278354635615 38 20.526601949318582 30.52284367868627 27.422974405852074 21.527579966143072 39 19.201080475729913 28.77725152361591 29.559881598228955 22.461786402425222 40 22.90059428331295 27.66845337957342 28.873374439644017 20.55757789746961 41 19.527898390225467 26.39047890332147 29.29907711169862 24.782545594754453 42 20.491618623259907 27.983303314101533 28.778388752481597 22.74668931015697 43 21.190797760633902 27.33925269983548 28.507565821182133 22.962383718348484 44 20.624078709234407 28.240154576479725 29.139919224258282 21.995847490027586 45 19.936055245495222 30.141817854926423 27.698725328902835 22.223401570675524 46 21.427882902251387 29.609703053297043 28.040760448696357 20.921653595755213 47 20.341504412989536 28.592370602308897 29.368556380016397 21.697568604685166 48 20.518153963459213 28.63764314191425 29.340233965885297 21.50396892874124 49 21.302625265759556 27.05575778974696 30.296210211881984 21.3454067326115 50 19.592612128058466 29.02717110528898 29.32333799416656 22.056878772485994 51 18.917802180663426 29.338284430686983 28.820574528022945 22.923338860626647 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1084.0 1 2336.5 2 3589.0 3 7006.0 4 10423.0 5 7686.0 6 4949.0 7 5081.5 8 5214.0 9 5383.5 10 5553.0 11 5725.0 12 5897.0 13 6087.0 14 6277.0 15 6032.5 16 5788.0 17 5616.0 18 5444.0 19 5561.5 20 5679.0 21 7137.5 22 8596.0 23 10521.0 24 12446.0 25 15391.5 26 21315.0 27 24293.0 28 29175.5 29 34058.0 30 40164.0 31 46270.0 32 51666.0 33 57062.0 34 65539.5 35 74017.0 36 78601.0 37 83185.0 38 85873.5 39 88562.0 40 105663.0 41 122764.0 42 130226.0 43 137688.0 44 144892.5 45 152097.0 46 152411.5 47 152726.0 48 151563.0 49 150400.0 50 147945.0 51 145490.0 52 128130.5 53 110771.0 54 103781.5 55 96792.0 56 88877.0 57 80962.0 58 72311.5 59 63661.0 60 55702.0 61 47743.0 62 40108.5 63 32474.0 64 25766.5 65 19059.0 66 15423.0 67 11787.0 68 9758.0 69 7729.0 70 5811.0 71 3893.0 72 2842.5 73 1792.0 74 1331.0 75 788.0 76 706.0 77 437.0 78 168.0 79 168.5 80 169.0 81 121.5 82 74.0 83 47.0 84 20.0 85 13.5 86 7.0 87 6.0 88 5.0 89 5.0 90 5.0 91 5.0 92 5.0 93 5.5 94 6.0 95 6.0 96 6.0 97 3.5 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1846594.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 16.447737282157515 #Duplication Level Percentage of deduplicated Percentage of total 1 77.72802753459524 12.78450176349328 2 9.094539669888563 2.9916919838497322 3 3.0870728917014567 1.523260916807276 4 1.479750360275637 0.9735418067596644 5 0.8799169991333619 0.7236321815924979 6 0.5989327217011471 0.5910652833736819 7 0.39859865818414714 0.4589232207583349 8 0.3239603290385596 0.4262731505494026 9 0.25953755087969904 0.38419249065514915 >10 2.9553181847742627 11.529298092874214 >50 1.3397791067620324 16.200185674787452 >100 1.8412381742153163 49.18908457244407 >500 0.008663082252912837 0.9360372170075799 >1k 0.004331541126456419 0.9833323058997077 >5k 3.3319547126587837E-4 0.3049793391479615 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5565 0.3013656494064207 No Hit AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT 2033 0.11009458494937166 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC 1983 0.10738689717393211 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.04207746803033043 0.0 2 0.0 0.0 0.0 0.1721006350069371 0.0 3 0.0 0.0 0.0 0.25170665560485955 0.0 4 0.0 0.0 0.0 0.3835168965132563 0.0 5 0.0 0.0 0.0 0.6850450071862033 0.0 6 0.0 0.0 0.0 0.92299660889183 0.0 7 0.0 0.0 0.0 1.0795551160677441 0.0 8 0.0 0.0 0.0 1.4071311831404196 0.0 9 0.0 0.0 0.0 1.5075322458537177 0.0 10 0.0 0.0 0.0 1.716890664650703 0.0 11 0.0 0.0 0.0 2.0674279240591056 0.0 12 0.0 0.0 0.0 2.35162683296924 0.0 13 0.0 0.0 0.0 2.4636709531169276 0.0 14 0.0 0.0 0.0 2.5137090232070505 0.0 15 0.0 0.0 0.0 2.602358720974941 0.0 16 0.0 0.0 0.0 2.7792790402221605 0.0 17 0.0 0.0 0.0 2.9922657606382344 0.0 18 0.0 0.0 0.0 3.2190075349535414 0.0 19 0.0 0.0 0.0 3.3710171266667173 0.0 20 0.0 0.0 0.0 3.5433885304511983 0.0 21 0.0 0.0 0.0 3.7490644938735858 0.0 22 0.0 0.0 0.0 3.9700659701049608 0.0 23 0.0 0.0 0.0 4.19133821511388 0.0 24 0.0 0.0 0.0 4.365605000341168 0.0 25 0.0 0.0 0.0 4.516802285721712 0.0 26 0.0 0.0 0.0 4.666699880970046 0.0 27 0.0 0.0 0.0 4.859649711847867 0.0 28 0.0 0.0 0.0 5.034403880874735 0.0 29 0.0 0.0 0.0 5.246957371246738 0.0 30 0.0 0.0 0.0 5.477219139670117 0.0 31 0.0 0.0 0.0 5.6840864857136975 0.0 32 0.0 0.0 0.0 5.864201876535936 0.0 33 5.415375550879078E-5 0.0 0.0 6.0605092402553025 0.0 34 5.415375550879078E-5 0.0 0.0 6.2691636602306735 0.0 35 5.415375550879078E-5 0.0 0.0 6.491573134105277 0.0 36 5.415375550879078E-5 0.0 0.0 6.703910009455246 0.0 37 5.415375550879078E-5 0.0 0.0 6.937258541942625 0.0 38 5.415375550879078E-5 0.0 0.0 7.205807015510719 0.0 39 5.415375550879078E-5 0.0 0.0 7.772905143198776 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACGTTG 20 7.034399E-4 45.000004 26 CGAACGC 20 7.034399E-4 45.000004 42 CGTATTC 20 7.034399E-4 45.000004 34 GGTAATC 20 7.034399E-4 45.000004 9 CGTATCC 20 7.034399E-4 45.000004 20 GACACGC 30 2.16622E-6 45.000004 13 CGCCTAA 20 7.034399E-4 45.000004 20 CCGTATG 20 7.034399E-4 45.000004 5 CCCGTAC 20 7.034399E-4 45.000004 26 CTACGAT 20 7.034399E-4 45.000004 12 TCGTCGA 20 7.034399E-4 45.000004 40 CGTCGTT 20 7.034399E-4 45.000004 12 ACCGTCC 20 7.034399E-4 45.000004 35 TACGTCG 20 7.034399E-4 45.000004 10 GTACGAT 30 2.16622E-6 45.000004 11 GACTACG 30 2.16622E-6 45.000004 30 AACCGTC 35 1.2124656E-7 45.0 34 CCCTCGA 35 1.2124656E-7 45.0 20 AACCCGC 35 1.2124656E-7 45.0 21 TTTCGCA 45 3.8562575E-10 45.0 40 >>END_MODULE