FastQCFastQC Report
Thu 26 May 2016
SRR1547577_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547577_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2374509
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234130.9860143718132884No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTA253.8920658E-545.00000429
GCGATCG302.1664837E-645.0000049
GTACGCG302.1664837E-645.0000041
ACTATCG207.0348073E-445.024
TCCGCTA207.0348073E-445.040
GCGCGAA207.0348073E-445.021
TACGCTA207.0348073E-445.019
CGTAACG207.0348073E-445.044
GTGCGAT207.0348073E-445.028
TATCCGC207.0348073E-445.019
CGGACCG207.0348073E-445.03
CGACGGT3550.043.73239528
CGGTCTA3550.043.73239531
CGTTTTT105950.043.4072651
CACGACG3600.042.526
TATCGCG850.042.3529431
CGCGGAT603.6379788E-1241.2500044
TCGCGAG556.184564E-1140.9090921
GACGGTC3800.040.85526329
GCGTAAG1900.040.2631571
Produced by FastQC (version 0.11.5)