##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547577_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2374509
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.575753555787745	31.0	31.0	33.0	30.0	34.0
2	32.0028831223634	31.0	31.0	34.0	30.0	34.0
3	32.0943681409504	33.0	31.0	34.0	30.0	34.0
4	35.84002798052145	37.0	35.0	37.0	35.0	37.0
5	35.778888182778	37.0	35.0	37.0	35.0	37.0
6	35.82556857017598	37.0	35.0	37.0	35.0	37.0
7	36.132224388284065	37.0	35.0	37.0	35.0	37.0
8	36.11121414995689	37.0	35.0	37.0	35.0	37.0
9	37.80802136357453	39.0	38.0	39.0	35.0	39.0
10	37.41481165158776	39.0	37.0	39.0	35.0	39.0
11	37.13744946850065	39.0	37.0	39.0	33.0	39.0
12	36.94178291175144	39.0	37.0	39.0	33.0	39.0
13	36.87878925706325	39.0	37.0	39.0	33.0	39.0
14	37.986175247177414	40.0	37.0	41.0	33.0	41.0
15	38.16703958586807	40.0	37.0	41.0	33.0	41.0
16	38.224416500421775	40.0	37.0	41.0	33.0	41.0
17	38.16054224262784	40.0	37.0	41.0	33.0	41.0
18	38.030089167907974	40.0	37.0	41.0	33.0	41.0
19	37.995540551751965	40.0	37.0	41.0	33.0	41.0
20	37.876565639464836	40.0	36.0	41.0	33.0	41.0
21	37.75687142057579	40.0	35.0	41.0	33.0	41.0
22	37.71176904362123	40.0	35.0	41.0	33.0	41.0
23	37.61435901064178	39.0	35.0	41.0	33.0	41.0
24	37.586280363645706	39.0	35.0	41.0	33.0	41.0
25	37.5202279713406	39.0	35.0	41.0	33.0	41.0
26	37.54871596612184	39.0	35.0	41.0	33.0	41.0
27	37.525621086296155	39.0	35.0	41.0	33.0	41.0
28	37.474092117570414	39.0	35.0	41.0	33.0	41.0
29	37.42196091907843	39.0	35.0	41.0	33.0	41.0
30	37.319052486219256	39.0	35.0	41.0	32.0	41.0
31	37.168602014142714	39.0	35.0	41.0	32.0	41.0
32	37.03146039876033	39.0	35.0	41.0	31.0	41.0
33	36.88079598771788	39.0	35.0	41.0	31.0	41.0
34	36.76018873796646	39.0	35.0	41.0	31.0	41.0
35	36.62961311159486	39.0	35.0	41.0	30.0	41.0
36	36.57395907954023	39.0	35.0	41.0	30.0	41.0
37	36.537017126488045	39.0	35.0	41.0	30.0	41.0
38	36.40230338145697	39.0	35.0	41.0	30.0	41.0
39	36.32490969712054	39.0	35.0	41.0	30.0	41.0
40	36.27046559941445	39.0	35.0	41.0	30.0	41.0
41	36.217894309939446	39.0	35.0	41.0	30.0	41.0
42	36.21366059256882	39.0	35.0	41.0	30.0	41.0
43	36.19308581268801	39.0	35.0	41.0	30.0	41.0
44	36.08544545419706	39.0	35.0	41.0	29.0	41.0
45	36.03805839438806	39.0	35.0	41.0	29.0	41.0
46	36.02237304638559	39.0	35.0	41.0	29.0	41.0
47	35.97085839640953	39.0	35.0	41.0	29.0	41.0
48	35.88160920847215	38.0	35.0	40.0	29.0	41.0
49	35.81339131584677	38.0	35.0	40.0	28.0	41.0
50	35.71096593021968	38.0	35.0	40.0	28.0	41.0
51	35.28768894958916	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	3.0
12	4.0
13	9.0
14	11.0
15	44.0
16	104.0
17	240.0
18	499.0
19	938.0
20	1774.0
21	3037.0
22	4954.0
23	7742.0
24	12300.0
25	19961.0
26	27536.0
27	31467.0
28	33353.0
29	36202.0
30	42466.0
31	52213.0
32	67209.0
33	89498.0
34	145948.0
35	195962.0
36	173114.0
37	231615.0
38	383684.0
39	812355.0
40	263.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.09050797449073	23.63739198293205	29.750445249944303	14.521654792632917
2	31.51106186584258	24.97484743161639	31.077245864302895	12.436844838238137
3	28.59285856570769	24.847368445434405	33.91421131695015	12.645561671907751
4	25.863199507771924	26.750835646443115	33.81237973829537	13.573585107489592
5	23.593172314781707	31.43125589332363	31.58080259961112	13.394769192283542
6	22.321793684504883	39.75377646494496	27.423479969964315	10.50094988058584
7	88.2232495223223	2.7871446265312114	7.557099172923749	1.43250667822274
8	89.00286332879766	2.071207142192344	7.181358335554846	1.7445711934551522
9	83.76573851688917	5.480627784523032	8.342019339577151	2.411614359010642
10	38.56666788797179	40.6602375480573	13.23570472885131	7.537389835119597
11	24.109658038777702	24.932438664161726	33.94727078313874	17.010632513921824
12	26.250311116950915	22.97413907464659	32.48739002463246	18.288159783770034
13	25.650818758741277	24.220923146637894	31.903100809472612	18.225157285148214
14	17.344133039714738	29.34375064487016	33.29138781954501	20.020728495870095
15	16.663529175926474	30.99386020436225	35.87230033661696	16.470310283094317
16	23.28106568557963	29.835010101035625	32.34630822624804	14.537615987136709
17	22.873276117294143	29.312080939680584	29.727619478384792	18.087023464640477
18	21.926890990937494	29.77849315374252	31.688403792110286	16.6062120632097
19	22.57649897305085	30.760675154316115	29.769817675991124	16.89300819664192
20	23.978515137234687	31.321422660432113	29.917342911734597	14.782719290598603
21	25.488300949796354	26.24197255095685	32.9534232129674	15.316303286279394
22	23.35367016928552	22.685911066245694	34.51319830752379	19.447220456944994
23	19.91548568567228	25.76048353575413	35.338758454905836	18.985272323667758
24	19.19415761321604	28.03278488310636	34.98361134870409	17.78944615497351
25	20.614661810083685	30.71712930968044	31.213442442205945	17.454766438029925
26	19.75962188393474	33.181386130774825	28.628529098015633	18.4304628872748
27	18.463185441706052	31.894383217751543	32.48701099890546	17.155420341636944
28	17.554450204231696	30.367372791596075	33.911726592739804	18.16645041143243
29	18.54122262749899	28.88908822834531	34.23545667756998	18.334232466585725
30	20.491057309111063	27.8394396483652	33.13733491850315	18.532168124020586
31	21.419038630723236	28.630003086953977	31.56884223222569	18.382116050097093
32	22.275931571537527	28.606419263940463	31.5697266255887	17.54792253893331
33	21.760877722510212	29.121094087240774	30.68006901637349	18.437959173875527
34	19.499694463150067	29.05392230562192	32.500950722865234	18.94543250836278
35	19.852104161323457	28.77348538160942	32.21840810036938	19.156002356697744
36	22.116740766196298	28.969483796439597	30.774741220184886	18.139034217179216
37	20.885201951224442	30.41643556625812	30.380765033950176	18.31759744856726
38	21.010659466862414	29.898812765081118	29.974280998724367	19.1162467693321
39	20.725842690004544	29.26765070168191	30.08693586758357	19.919570740729977
40	21.538600190607827	28.795468873775587	30.715865890590432	18.95006504502615
41	19.733258538923202	28.47780320057747	30.75427383092673	21.034664429572597
42	20.4441844608717	28.718989904860333	30.9789939730698	19.857831661198166
43	21.203962587633907	28.058895544299894	29.976428811177385	20.760713056888815
44	20.355618782662017	29.402373290646615	30.252106856617516	19.989901070073856
45	19.940711953502806	29.733346978259505	29.564427845925202	20.761513222312487
46	21.26553321128705	28.861672034092102	30.02031156757039	19.85248318705046
47	19.90613638440621	29.069799272186376	31.245449059152865	19.778615284254556
48	19.866422911010233	28.79921701707595	31.333635711635544	20.000724360278273
49	21.453656313789505	27.746536231279812	31.021781766251465	19.778025688679218
50	19.666044643334686	28.527245000966516	31.44191072764938	20.364799628049422
51	19.23964070045639	29.120125465938436	30.945050113518207	20.695183720086973
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1710.0
1	2799.5
2	3889.0
3	19790.0
4	35691.0
5	24465.0
6	13239.0
7	12773.0
8	12307.0
9	12838.5
10	13370.0
11	13526.5
12	13683.0
13	13813.5
14	13944.0
15	13449.5
16	12955.0
17	12362.5
18	11770.0
19	12184.0
20	12598.0
21	13628.5
22	14659.0
23	16490.5
24	18322.0
25	23063.5
26	35318.5
27	42832.0
28	47130.5
29	51429.0
30	62317.0
31	73205.0
32	76773.0
33	80341.0
34	93032.5
35	105724.0
36	113228.0
37	120732.0
38	127244.0
39	133756.0
40	146359.5
41	158963.0
42	169239.5
43	179516.0
44	182581.0
45	185646.0
46	192002.0
47	198358.0
48	194615.5
49	190873.0
50	178137.5
51	165402.0
52	146117.0
53	126832.0
54	113742.5
55	100653.0
56	93856.0
57	87059.0
58	75242.5
59	63426.0
60	53284.0
61	43142.0
62	35353.0
63	27564.0
64	22056.5
65	16549.0
66	12257.5
67	7966.0
68	6061.0
69	4156.0
70	3312.5
71	2469.0
72	1763.5
73	1058.0
74	767.5
75	397.0
76	317.0
77	194.0
78	71.0
79	52.5
80	34.0
81	19.5
82	5.0
83	5.5
84	6.0
85	3.5
86	1.0
87	1.5
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2374509.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.372220764426082
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.71662468465097	13.910825442436009
2	9.053898123102346	3.3268043019251845
3	3.2783661222684537	1.8069259843479446
4	1.736739304881842	1.276310316781804
5	1.0561259830853063	0.9701689858144884
6	0.6852173216816585	0.7553378343326514
7	0.5257401967300855	0.6761310471340551
8	0.40561051038274143	0.596157267289861
9	0.3453873529145469	0.5710979427288119
>10	4.246270615969535	18.91399451530771
>50	1.7013054828207388	22.71144757938767
>100	1.242905247530102	32.594438957104074
>500	0.003950156707541742	0.5296132425652639
>1k	0.001626535114870129	0.3612435320479818
>5k	0.0	0.0
>10k+	2.323621592671613E-4	0.999503050796431
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23413	0.9860143718132884	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06338152434882327	0.0
2	0.0	0.0	0.0	0.21448644751399132	0.0
3	0.0	0.0	0.0	0.3255830994955168	0.0
4	0.0	0.0	0.0	0.4726871955423205	0.0
5	0.0	0.0	0.0	0.8390366176754858	0.0
6	0.0	0.0	0.0	1.1922885952422164	0.0
7	0.0	0.0	0.0	1.4227362372599976	0.0
8	0.0	0.0	0.0	1.8629114482194002	0.0
9	0.0	0.0	0.0	2.042443300909788	0.0
10	0.0	0.0	0.0	2.3343352246717113	0.0
11	0.0	0.0	0.0	2.7770372738111333	0.0
12	0.0	0.0	0.0	3.0941133514339176	0.0
13	0.0	0.0	0.0	3.2180547641638757	0.0
14	0.0	0.0	0.0	3.27861465254501	0.0
15	4.211396966699221E-5	0.0	0.0	3.3630531617273296	0.0
16	4.211396966699221E-5	0.0	0.0	3.5323092058189713	0.0
17	4.211396966699221E-5	0.0	0.0	3.7704637042858122	0.0
18	4.211396966699221E-5	0.0	0.0	4.0194836069267375	0.0
19	8.422793933398442E-5	0.0	0.0	4.199857739010465	0.0
20	8.422793933398442E-5	0.0	0.0	4.389075804724261	0.0
21	8.422793933398442E-5	0.0	0.0	4.616701810774353	0.0
22	8.422793933398442E-5	0.0	0.0	4.8999182567848765	0.0
23	8.422793933398442E-5	0.0	0.0	5.165025695838592	0.0
24	8.422793933398442E-5	0.0	0.0	5.363129809152124	0.0
25	8.422793933398442E-5	0.0	0.0	5.539166202360151	0.0
26	8.422793933398442E-5	0.0	0.0	5.7003363642757305	0.0
27	8.422793933398442E-5	0.0	0.0	5.886185312416167	0.0
28	8.422793933398442E-5	0.0	0.0	6.070812955436261	0.0
29	8.422793933398442E-5	0.0	0.0	6.284751921344581	0.0
30	8.422793933398442E-5	0.0	0.0	6.5313292137448204	0.0
31	8.422793933398442E-5	0.0	0.0	6.761608399883934	0.0
32	8.422793933398442E-5	0.0	0.0	6.960133652894135	0.0
33	8.422793933398442E-5	0.0	0.0	7.165902508687059	0.0
34	8.422793933398442E-5	0.0	0.0	7.385737430348758	0.0
35	8.422793933398442E-5	0.0	0.0	7.613447664338185	0.0
36	8.422793933398442E-5	0.0	0.0	7.832187622788543	0.0
37	8.422793933398442E-5	0.0	0.0	8.069205044074375	0.0
38	8.422793933398442E-5	0.0	0.0	8.32045698710765	0.0
39	8.422793933398442E-5	0.0	0.0	8.692365453236858	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGTA	25	3.8920658E-5	45.000004	29
GCGATCG	30	2.1664837E-6	45.000004	9
GTACGCG	30	2.1664837E-6	45.000004	1
ACTATCG	20	7.0348073E-4	45.0	24
TCCGCTA	20	7.0348073E-4	45.0	40
GCGCGAA	20	7.0348073E-4	45.0	21
TACGCTA	20	7.0348073E-4	45.0	19
CGTAACG	20	7.0348073E-4	45.0	44
GTGCGAT	20	7.0348073E-4	45.0	28
TATCCGC	20	7.0348073E-4	45.0	19
CGGACCG	20	7.0348073E-4	45.0	3
CGACGGT	355	0.0	43.732395	28
CGGTCTA	355	0.0	43.732395	31
CGTTTTT	10595	0.0	43.407265	1
CACGACG	360	0.0	42.5	26
TATCGCG	85	0.0	42.352943	1
CGCGGAT	60	3.6379788E-12	41.250004	4
TCGCGAG	55	6.184564E-11	40.909092	1
GACGGTC	380	0.0	40.855263	29
GCGTAAG	190	0.0	40.263157	1
>>END_MODULE