FastQCFastQC Report
Thu 26 May 2016
SRR1547576_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547576_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2152285
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105330.489386860940814No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG27990.13004783288458546No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAT207.0346624E-445.015
CCGCTAT551.8189894E-1245.038
CCTCGCG207.0346624E-445.012
GTTACGC207.0346624E-445.045
TCGTACT207.0346624E-445.033
CGCGTCA207.0346624E-445.039
TCGACGT551.8189894E-1245.026
TACCGCG406.8193913E-945.01
ACGGTCG207.0346624E-445.036
CTAGCGT207.0346624E-445.014
CGTACGA302.166389E-644.99999636
CGTGTCG302.166389E-644.99999637
CTTACCG302.166389E-644.99999632
TACGCGG1100.042.9545442
CGTTTTT51000.042.26471
CGAATAT2200.040.90909214
GCCCGTT556.184564E-1140.9090923
TACGTAG1100.040.9090921
TCGATAG1050.040.7142871
TTTACGG2550.040.5882342